BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	499,479	A→G	N186N (AAT→AAC)	unknown ←	Spermidine/putrescine import ABC transporter substrate‑binding protein PotD (TC 3.A.1.11.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	499,479	0	A	G	100.0%	323.6 / NA	112	N186N (AAT→AAC)	unknown	Spermidine/putrescine import ABC transporter substrate‑binding protein PotD (TC 3.A.1.11.1)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (56/56); total (56/56)

Only 100 of 113 total aligned reads displayed.

TTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTATTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTTGTGCAATAAATTGTCCGGAATTTGTTTGAAATTGCTTAAC  >  1/499245‑499725

tttCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGt                                                                                                                                                                                                                                        >  1:310730/1‑251 (MQ=255)

tttCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGt                                                                                                                                                                                                                                        >  1:7405/1‑251 (MQ=255)

tttCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGt                                                                                                                                                                                                                                        >  1:196755/1‑251 (MQ=255)

     gAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCcaa                                                                                                                                                                                                                                    >  1:354215/1‑250 (MQ=255)

       aTAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCcaacaa                                                                                                                                                                                                                                 <  1:750710/251‑1 (MQ=255)

       aTAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCcaacaa                                                                                                                                                                                                                                 >  1:705371/1‑251 (MQ=255)

        tAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAAt                                                                                                                                                                                                                                <  1:576221/251‑1 (MQ=255)

          gCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATaa                                                                                                                                                                                                                              >  1:527358/1‑251 (MQ=255)

             tAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGCTTTTCCGTCCAACAATAACg                                                                                                                                                                                                                            >  1:664192/1‑250 (MQ=255)

             tAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACg                                                                                                                                                                                                                            >  1:272013/1‑250 (MQ=255)

             tAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACg                                                                                                                                                                                                                            >  1:48212/1‑250 (MQ=255)

                            aaaaTTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAacac                                                                                                                                                                                                               <  1:554386/248‑1 (MQ=255)

                                        tcttTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCa                                                                                                                                                                                                  >  1:867399/1‑249 (MQ=255)

                                         cttTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTa                                                                                                                                                                                               >  1:228329/1‑251 (MQ=255)

                                          tttCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAg                                                                                                                                                                                              <  1:198071/251‑1 (MQ=255)

                                          tttCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAg                                                                                                                                                                                              <  1:525718/251‑1 (MQ=255)

                                            tCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTg                                                                                                                                                                                            <  1:563014/251‑1 (MQ=255)

                                                   gACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAaca                                                                                                                                                                                     >  1:301757/1‑251 (MQ=255)

                                                    aCGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAacac                                                                                                                                                                                    <  1:870357/251‑1 (MQ=255)

                                                       aaCTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAac                                                                                                                                                                                                                 <  1:408770/219‑1 (MQ=255)

                                                       aaCTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACttt                                                                                                                                                                                 >  1:380110/1‑251 (MQ=255)

                                                       aaCTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACttt                                                                                                                                                                                 >  1:694453/1‑251 (MQ=255)

                                                          tGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGcc                                                                                                                                                                              <  1:55733/251‑1 (MQ=255)

                                                             aTGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCttt                                                                                                                                                                           >  1:81862/1‑251 (MQ=255)

                                                                ttttcgttttcACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTa                                                                                                                                                                        <  1:676403/251‑1 (MQ=255)

                                                                   tcgttttcACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTc                                                                                                                                                                     <  1:212166/251‑1 (MQ=255)

                                                                      ttttcACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCgg                                                                                                                                                                  <  1:211336/251‑1 (MQ=255)

                                                                              gtgtGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTg                                                                                                                                                                                            <  1:339694/217‑1 (MQ=255)

                                                                              gtgtGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCcaca                                                                                                                                                          >  1:318807/1‑251 (MQ=255)

                                                                              gtgtGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCcac                                                                                                                                                           <  1:257417/250‑1 (MQ=255)

                                                                                gtgCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGATGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCgg                                                                                                                                                                  >  1:626188/1‑241 (MQ=255)

                                                                                     aTGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAg                                                                                                                                                                                              >  1:157606/1‑208 (MQ=255)

                                                                                     aTGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTg                                                                                                                                                                                            <  1:727656/210‑1 (MQ=255)

                                                                                     aTGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGa                                                                                                                                                   >  1:51770/1‑251 (MQ=255)

                                                                                     aTGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGa                                                                                                                                                   >  1:830173/1‑251 (MQ=255)

                                                                                       gTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAac                                                                                                                                                                                                                 <  1:52157/187‑1 (MQ=255)

                                                                                        tAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGAcc                                                                                                                                                 <  1:549725/250‑1 (MQ=255)

                                                                                         aGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAg                                                                                                                                               >  1:244814/1‑251 (MQ=255)

                                                                                              gAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCgcgcgc                                                                                                                                                                                                      >  1:888399/1‑191 (MQ=255)

                                                                                                   gTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAAt                                                                                                                                                                                                                                <  1:787018/160‑1 (MQ=255)

                                                                                                    ttCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAacac                                                                                                                                                                                    >  1:610786/1‑203 (MQ=255)

                                                                                                       cAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCt                                                                                                                                 <  1:691112/251‑1 (MQ=255)

                                                                                                        aaaTCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGa                                                                                                                                        <  1:266140/243‑1 (MQ=255)

                                                                                                          aTCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAAc                                                                                                                                                                                                                             <  1:666377/156‑1 (MQ=255)

                                                                                                          aTCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGa                                                                                                                               >  1:561198/1‑250 (MQ=255)

                                                                                                               gCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACt                                                                                                                                                                                                <  1:641886/180‑1 (MQ=255)

                                                                                                               gCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAg                                                                                                                                                                                              <  1:459597/182‑1 (MQ=255)

                                                                                                               gCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTg                                                                                                                                                                                            >  1:787597/1‑184 (MQ=255)

                                                                                                                    gTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTc                                                                                                                                                                                                                                           >  1:729566/1‑132 (MQ=255)

                                                                                                                    gTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACg                                                                                                                             <  1:281241/242‑1 (MQ=255)

                                                                                                                    gTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACAt                                                                                                                      >  1:61118/1‑249 (MQ=255)

                                                                                                                    gTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACAt                                                                                                                      >  1:473575/1‑249 (MQ=255)

                                                                                                                      gCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGAt                                                                                                                                       >  1:284947/1‑230 (MQ=255)

                                                                                                                         aCTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAac                                                                                                                                                                                      <  1:608039/180‑1 (MQ=255)

                                                                                                                         aCTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGAt                                                                                                                                       >  1:288499/1‑227 (MQ=255)

                                                                                                                              gCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAacac                                                                                                                                                                                    <  1:676846/177‑1 (MQ=255)

                                                                                                                              gCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGCTCGTAAGCAGCTTTGACTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATc                                                                                                                                                      >  1:27056/1‑207 (MQ=255)

                                                                                                                               cccTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCa                                                                                                          >  1:621305/1‑250 (MQ=255)

                                                                                                                                  tGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTCCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGc                                                                                                      <  1:211549/251‑1 (MQ=255)

                                                                                                                                   gCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACtt                                                                                                                                                                                  >  1:235177/1‑174 (MQ=255)

                                                                                                                                   gCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTAt                                                                                                                                                                       >  1:240407/1‑185 (MQ=255)

                                                                                                                                   gCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCg                                                                                                     >  1:430580/1‑251 (MQ=255)

                                                                                                                                     acctAAGGCCCTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAac                                                                                                                                                                                                                 <  1:701611/138‑1 (MQ=255)

                                                                                                                                      caTAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATa                                                                                                  >  1:52291/1‑251 (MQ=255)

                                                                                                                                      caTAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACCAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATa                                                                                                  >  1:891144/1‑251 (MQ=255)

                                                                                                                                       aTAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATAc                                                                                                 <  1:864655/251‑1 (MQ=255)

                                                                                                                                         aaGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCg                                                                                               <  1:611840/251‑1 (MQ=255)

                                                                                                                                            gCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGtt                                                                                            >  1:663325/1‑251 (MQ=255)

                                                                                                                                                 tCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGAt                                                                                                                                       >  1:171085/1‑203 (MQ=255)

                                                                                                                                                 tCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAAcc                                                                                       <  1:69688/251‑1 (MQ=255)

                                                                                                                                                      cGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATac                                                                                   <  1:220331/250‑1 (MQ=255)

                                                                                                                                                        aGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATacac                                                                                 <  1:114909/250‑1 (MQ=255)

                                                                                                                                                        aGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATacac                                                                                 <  1:383730/250‑1 (MQ=255)

                                                                                                                                                        aGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATacac                                                                                 <  1:388730/250‑1 (MQ=255)

                                                                                                                                                        aGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATacac                                                                                 <  1:879766/250‑1 (MQ=255)

                                                                                                                                                        aGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATacac                                                                                 <  1:117952/250‑1 (MQ=255)

                                                                                                                                                          tCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACAt                                                                               <  1:646708/250‑1 (MQ=255)

                                                                                                                                                              aGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAgg                                                                           <  1:669739/250‑1 (MQ=255)

                                                                                                                                                              aGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAgg                                                                           <  1:455093/250‑1 (MQ=255)

                                                                                                                                                               gTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATAc                                                                                                 >  1:670575/1‑227 (MQ=255)

                                                                                                                                                               gTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCa                                                                         >  1:474244/1‑251 (MQ=255)

                                                                                                                                                                ttAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACGCTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGc                                                                          <  1:435636/249‑1 (MQ=255)

                                                                                                                                                                ttAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGc                                                                          <  1:837217/249‑1 (MQ=255)

                                                                                                                                                                ttAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGc                                                                          <  1:549961/249‑1 (MQ=255)

                                                                                                                                                                    aaaCGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGaa                                                                    >  1:408436/1‑251 (MQ=255)

                                                                                                                                                                               ttCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTAtt                                                         <  1:27261/251‑1 (MQ=255)

                                                                                                                                                                                          tttttCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATacac                                                                                 >  1:4867/1‑216 (MQ=255)

                                                                                                                                                                                               ccAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTtg                                         <  1:271649/251‑1 (MQ=255)

                                                                                                                                                                                                      ttCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGt                                                                                                                                                                >  1:498812/1‑125 (MQ=255)

                                                                                                                                                                                                      ttCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGc                                                                          >  1:148314/1‑211 (MQ=255)

                                                                                                                                                                                                      ttCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTc                                                        >  1:498662/1‑229 (MQ=255)

                                                                                                                                                                                                      ttCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTTGTGCAATa                                  >  1:304646/1‑251 (MQ=255)

                                                                                                                                                                                                           aaGCAGCTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTTGTGCAATAAATTg                             <  1:88658/251‑1 (MQ=255)

                                                                                                                                                                                                                gcTTTGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTTGTGCAATAAATTGTCCgg                        >  1:782322/1‑251 (MQ=255)

                                                                                                                                                                                                                   ttGATTTCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTTGTGCAATAAATTGTCCGGAAt                     <  1:630970/251‑1 (MQ=255)

                                                                                                                                                                                                                        ttCGTCTTCGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTTGTGCAATAAATTGTCCGGAAtttgtt                <  1:690268/251‑1 (MQ=255)

                                                                                                                                                                                                                                cGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGc                                                                          >  1:583820/1‑185 (MQ=255)

                                                                                                                                                                                                                                cGTTGGTGGTGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTAtt                                                         <  1:584781/202‑1 (MQ=255)

                                                                                                                                                                                                                                     gtggtgTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTTGTGCAATAAATTGTCCGGAATTTGTTTGAAATTGCTTaa   >  1:272887/1‑251 (MQ=255)

                                                                                                                                                                                                                                      tggtGTTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACTCTTTGCCTTTGTATTCCGGGTTCCACAATTCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATGCCGGTTAAACCATACGCATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTTGTGCAATAAATTGTCCGGAATTTGTTTGAAATTGCTTAAc  <  1:146236/251‑1 (MQ=255)

TTTCGGAATAGCGTAGTTATCCATCCAGAAAATTGCGCCTTCTTTCGGATAGACGAACTGAATGTTTTCGTTTTCACGGTGTGCCATGTAGGCGGAACCGTTCCAAATCATGCCTAGTGCTACTTCGCCCTGCACATAAGGCACTTCCGGCGAGTCGGAGTTAAAAACGGCGACGTTCGGCAATAATTTTTCCAACCGTTCGTAAGCAGCTTTGATTTCGTCTTCGTTGGTGGTATTCGGTGATTTTCCGTCCAACAATAACGCCACATGAAACACTTCGCGCGCATCACTAGTGAGCAACACTTTGCCTTTGTATTCCGGGTTCCACAAATCTGACCAGCTAGTGATTTTGCTGACGTCAACATCTTCCGAGTTCACGGCGATACCGGTTAAACCATACACATAAGGCAGGGAATAGTCGTTATTCGGGTCGAAATCTTTGTGCAATAAATTGTCCGGAATTTGTTTGAAATTGCTTAAC  >  1/499245‑499725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`