Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	324,799	A→G	M587T (ATG→ACG)	unknown ←	Mobile element protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	324,799	0	A	G	100.0%	217.9 / NA	74	M587T (ATG→ACG)	unknown	Mobile element protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/45);  total (29/45)

TAAAAACTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCATCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCAAATCTTGCCGATATTACGAGGATCTCGACGGAATAAAAATTCCTTGCTC  >  1/324559‑325044                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      tAAAAACTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGc                                                                                                                                                                                                                                             <  1:319611/251‑1 (MQ=255)   aaaaCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAg                                                                                                                                                                                                                                           <  1:543094/251‑1 (MQ=255)   aaaaCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAg                                                                                                                                                                                                                                           <  1:871294/251‑1 (MQ=255)   aaaaCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTATTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAg                                                                                                                                                                                                                                           <  1:329304/251‑1 (MQ=255)     aaCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGAc                                                                                                                                                                                                                                         <  1:465193/251‑1 (MQ=255)     aaCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGAc                                                                                                                                                                                                                                         <  1:314081/251‑1 (MQ=255)      aCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACt                                                                                                                                                                                                                                        <  1:381811/251‑1 (MQ=255)      aCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACt                                                                                                                                                                                                                                        <  1:702228/251‑1 (MQ=255)      aCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACt                                                                                                                                                                                                                                        <  1:344861/251‑1 (MQ=255)      aCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACt                                                                                                                                                                                                                                        <  1:591481/251‑1 (MQ=255)      aCTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACt                                                                                                                                                                                                                                        <  1:570002/251‑1 (MQ=255)       cTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTg                                                                                                                                                                                                                                       <  1:600303/251‑1 (MQ=255)              cGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAAcc                                                                                                                                                                                                                                <  1:88524/251‑1 (MQ=255)                                               aaTTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGAt                                                                                                                                                                                                <  1:206745/250‑1 (MQ=255)                                                  ttttCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATctgc                                                                                                                                                                                            <  1:18148/251‑1 (MQ=255)                                                  ttttCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATctgc                                                                                                                                                                                            <  1:176105/251‑1 (MQ=255)                                                        tGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAg                                                                                                                                                                                      <  1:359241/251‑1 (MQ=255)                                                           aCATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTg                                                                                                                                                                                                                                       <  1:353407/199‑1 (MQ=255)                                                            cATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGc                                                                                                                                                                                                                       <  1:218928/214‑1 (MQ=255)                                                              tACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGt                                                                                                                                                                                     >  1:39169/1‑246 (MQ=255)                                                                  cATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGAc                                                                                                                                                                                                                                         <  1:120058/190‑1 (MQ=255)                                                                  cATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGtt                                                                                                                                                                            >  1:846790/1‑251 (MQ=255)                                                                  cATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGtt                                                                                                                                                                            >  1:361956/1‑251 (MQ=255)                                                                        gTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACt                                                                                                                                                                                                                                        <  1:630498/185‑1 (MQ=255)                                                                                 ttattCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATc                                                                                                                                                                                               <  1:531589/217‑1 (MQ=255)                                                                                      cAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAg                                                                                                                                                        <  1:282612/251‑1 (MQ=255)                                                                                                   cGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACtt                                                                                                                                           >  1:262821/1‑251 (MQ=255)                                                                                                         aTCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACttgtttgt                                                                                                                                     >  1:394100/1‑251 (MQ=255)                                                                                                         aTCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACttgtttgt                                                                                                                                     <  1:685915/251‑1 (MQ=255)                                                                                                          tCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTa                                                                                                                                    >  1:238440/1‑251 (MQ=255)                                                                                                               aTCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATg                                                                                                                                                                                                          <  1:789835/176‑1 (MQ=255)                                                                                                               aTCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCg                                                                                                                                                                                        >  1:682727/1‑194 (MQ=255)                                                                                                               aTCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTcc                                                                                                                                >  1:568896/1‑250 (MQ=255)                                                                                                               aTCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAACTCACGCATTTCAGTTAATGCATCGTGGATc                                                                                                                                                                                               >  1:731558/1‑187 (MQ=255)                                                                                                                     aaGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGc                                                                                                                         <  1:709525/251‑1 (MQ=255)                                                                                                                           gTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACt                                                                                                                                                                                                                                        <  1:175862/134‑1 (MQ=255)                                                                                                                           gTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCatctttatc                                                                                                                                                               >  1:683646/1‑207 (MQ=255)                                                                                                                           gTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACg                                                                                                                                                >  1:257377/1‑222 (MQ=255)                                                                                                                           gTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCatat                                                                                                                   >  1:856007/1‑251 (MQ=255)                                                                                                                                   cAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATc                                                                                                                  >  1:894142/1‑244 (MQ=255)                                                                                                                                     ggTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTc                                                                                                                                                                                                                  <  1:250096/146‑1 (MQ=255)                                                                                                                                      gtgtTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCatc                                                                                                                                                                     >  1:228058/1‑190 (MQ=255)                                                                                                                                      gtgtTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGa                                                                                                         >  1:248637/1‑250 (MQ=255)                                                                                                                                       tgtTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGAc                                                                                                        <  1:733721/250‑1 (MQ=255)                                                                                                                                       tgtTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGAc                                                                                                        <  1:899875/250‑1 (MQ=255)                                                                                                                                       tgtTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGAc                                                                                                        >  1:200729/1‑250 (MQ=255)                                                                                                                                       tgtTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGCTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGAc                                                                                                        <  1:521473/250‑1 (MQ=255)                                                                                                                                                 gTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAAcc                                                                                                                                                                                                                                <  1:122032/120‑1 (MQ=255)                                                                                                                                                   ttCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACg                                                                                           >  1:611918/1‑251 (MQ=255)                                                                                                                                                       gTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATc                                                                                                                  >  1:328928/1‑224 (MQ=255)                                                                                                                                                       gTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAAt                                                                                       >  1:688051/1‑251 (MQ=255)                                                                                                                                                               gCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAAc                                                                                                                                                                                                                                 <  1:506320/105‑1 (MQ=255)                                                                                                                                                                  ttCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTTCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAaataat                                                                            <  1:450183/251‑1 (MQ=255)                                                                                                                                                                               gttgGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGAc                                                                                                        <  1:270173/210‑1 (MQ=255)                                                                                                                                                                                 tgGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGAt                                                             <  1:117541/251‑1 (MQ=255)                                                                                                                                                                                  gggATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGAt                                                             >  1:465080/1‑250 (MQ=255)                                                                                                                                                                                       cACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGa                                                       >  1:720085/1‑251 (MQ=255)                                                                                                                                                                                         cTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGt                                                                                                              <  1:161843/194‑1 (MQ=255)                                                                                                                                                                                         cTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGAc                                                                                                        <  1:48234/200‑1 (MQ=255)                                                                                                                                                                                         cTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGAt                                                             >  1:616293/1‑243 (MQ=255)                                                                                                                                                                                          tACCTTTCTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAAcc                                                    <  1:282375/251‑1 (MQ=255)                                                                                                                                                                                           accgtACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGAc                                                                                                        <  1:278597/194‑1 (MQ=255)                                                                                                                                                                                           aCCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCa                                                   >  1:661258/1‑251 (MQ=255)                                                                                                                                                                                           aCCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCa                                                   >  1:648539/1‑251 (MQ=255)                                                                                                                                                                                           aCCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCa                                                   >  1:741283/1‑251 (MQ=255)                                                                                                                                                                                           aCCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCa                                                   >  1:625862/1‑251 (MQ=255)                                                                                                                                                                                                      cgcgTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCAAATCTTGCCGa                                        >  1:857256/1‑251 (MQ=255)                                                                                                                                                                                                             aCTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCAAATCTTGCCGATATTACg                                 <  1:742705/251‑1 (MQ=255)                                                                                                                                                                                                              cTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCatctttatc                                                                                                                                                               <  1:176739/124‑1 (MQ=255)                                                                                                                                                                                                              cTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCAAATCTTGCCGATATTACGa                                <  1:267711/251‑1 (MQ=255)                                                                                                                                                                                                                             ccTGTCTCCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCAAATCTTGCCGATATTACGAGGATCTCGACGGAAt                 <  1:88731/251‑1 (MQ=255)                                                                                                                                                                                                                                  ctcCGAGCTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCAAATCTTGCCGATATTACGAGGATCTCGACGGAATaaaaa            >  1:380332/1‑251 (MQ=255)                                                                                                                                                                                                                                         cTTTCTTCGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCAAATCTTGCCGATATTACGAGGATCTCGACGGAATAAAAATTCCTTg     >  1:304453/1‑251 (MQ=255)                                                                                                                                                                                                                                            tcttcGTCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTGTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCAAATCTTGCCGATATTACGAGGATCTCGACGGAATAAAAATTCCTTGctc  <  1:451558/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      TAAAAACTCAATCCGTTCCTGATTGCTTGCAATCACTAAATGACGGAATTTTTCGTGAACATACTCATATTGTTTTTCCATTATTCAATATCTCCAAACGTGAAATCATTATCTAAAAGCAAGTTAGAAGCAGGTGTTTTGACTGTTTCTGTTGGTTTGCTTTCGGGTAAACTTGTTGGGATCACTACCTTACTTTTCGCGTGGACTTTCTGCCGTTGAGCCTGTCTCCGAGCTTTCTTCATCCGCTGTGCAGACTGTTCAACCAATTCACGCATTTCAGTTAATGCATCGTGGATCTGCTGCGAGTGAATGAGTTTTTCATCTTTATCTTTCAAGAAATGACGAACTTGTTTGTATTCCCAAATGCTCATATCAGGTAAAGACTGGTCGGCAAACGGAATCGGGAAATAATGTTTCAACTTCGGATCATAGAACCAAATCTTGCCGATATTACGAGGATCTCGACGGAATAAAAATTCCTTGCTC  >  1/324559‑325044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑