Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	86,562	T→G	K4T (AAG→ACG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	86,562	0	T	G	100.0%	143.7 / NA	50	K4T (AAG→ACG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/28);  total (22/28)

TGCGCTTACCACAACGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCTTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGATATATCCTTCTTTCTATTGTTACTGTTATTCGCCTTTCTGTCACATATTGCCCTAGGCATTTTAGATAATTATGCCAATC  >  1/86325‑86793                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                        tgCGCTTACCACAACGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAg                                                                                                                                                                                                                            <  1:209043/251‑1 (MQ=255)  gcgcTTACCACAACGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAgg                                                                                                                                                                                                                           >  1:460770/1‑251 (MQ=255)    gcTTACCACAACGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGaa                                                                                                                                                                                                                         >  1:757627/1‑251 (MQ=255)            caACGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAgg                                                                                                                                                                                                                           <  1:752329/241‑1 (MQ=255)            caACGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGa                                                                                                                                                                                                                   >  1:111742/1‑249 (MQ=255)            caACGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATa                                                                                                                                                                                                                 >  1:417078/1‑251 (MQ=255)            caACGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATa                                                                                                                                                                                                                 <  1:674398/251‑1 (MQ=255)              aCGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATaa                                                                                                                                                                                                                >  1:557482/1‑250 (MQ=255)               cGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAAcc                                                                                                                                                                                                              <  1:869020/251‑1 (MQ=255)               cGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAAcc                                                                                                                                                                                                              <  1:587135/251‑1 (MQ=255)               cGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAAcc                                                                                                                                                                                                              <  1:734154/251‑1 (MQ=255)               cGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAACTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAAcc                                                                                                                                                                                                              <  1:554304/251‑1 (MQ=255)                 gAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCt                                                                                                                                                                                                             <  1:253136/250‑1 (MQ=255)                           cATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGc                                                                                                                                                                                                   <  1:26187/250‑1 (MQ=255)                                         ctttctttTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTc                                                                                                                                                                                     <  1:663172/250‑1 (MQ=255)                                                tttCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAAt                                                                                                                                                                                          >  1:451785/1‑238 (MQ=255)                                                tttCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATAc                                                                                                                                                                                        >  1:407916/1‑240 (MQ=255)                                                  tCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTa                                                                                                                                                                           <  1:367163/251‑1 (MQ=255)                                                       aCCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACaaa                                                                                                                                                                      >  1:578189/1‑251 (MQ=255)                                                         cGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCGAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGaaa                                                                                                                                                                                            >  1:443874/1‑227 (MQ=255)                                                          gAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTa                                                                                                                                                                                                       <  1:101739/215‑1 (MQ=255)                                                                      ctgAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAg                                                                                                                                                                             >  1:777302/1‑229 (MQ=255)                                                                         aaaCAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGcc                                                                                                                                                     <  1:755672/250‑1 (MQ=255)                                                                         aaaCAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGcc                                                                                                                                                     <  1:517814/250‑1 (MQ=255)                                                                                aaTTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGaaa                                                                                                                                                                                            >  1:648015/1‑204 (MQ=255)                                                                                                  tttATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAgg                                                                                                                                                                                                                           <  1:141389/155‑1 (MQ=255)                                                                                                  tttATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGtaata                                                                                                                           <  1:357610/251‑1 (MQ=255)                                                                                                    tataACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAAc                                                                                                                         <  1:519759/251‑1 (MQ=255)                                                                                                          ctgctgAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGt                                                                                                                                                                                                        >  1:819363/1‑166 (MQ=255)                                                                                                              tgAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTtcaa                                                                                                               <  1:366243/251‑1 (MQ=255)                                                                                                                     tCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAacac                                                                                                                                       <  1:362995/220‑1 (MQ=255)                                                                                                                             cTTCCTTTAATCCGAGTTGAAAAACAAATATACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTATTGATAAACACATGACTTTTATAACAATGTTTCAGTCTAACAAATTctct                                                                                                <  1:491678/251‑1 (MQ=255)                                                                                                                                ccTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTtc                                                                                                                 <  1:340886/231‑1 (MQ=255)                                                                                                                                         cGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAAt                                                                                    <  1:533486/251‑1 (MQ=255)                                                                                                                                               gAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAAc                                                                                                                                                                                                                        >  1:636659/1‑113 (MQ=255)                                                                                                                                                       aaTTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGt                                                                                                                    <  1:512523/205‑1 (MQ=255)                                                                                                                                                        aTTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAAcc                                                                                                                                                                                                              >  1:287244/1‑114 (MQ=255)                                                                                                                                                                 ttGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAacac                                                                                                                                       >  1:842550/1‑176 (MQ=255)                                                                                                                                                                                    tttttCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGATATATCCTTCTTTCTATTGTTACTGTTATTCGCCTTTCTGt                                         <  1:687836/251‑1 (MQ=255)                                                                                                                                                                                                        ttattaAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAAcc                                                                                                                                                                                                              >  1:516388/1‑66 (MQ=255)                                                                                                                                                                                                          attaAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACa                                                                                                                        >  1:276595/1‑150 (MQ=255)                                                                                                                                                                                                          attaAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGttt                                                                                                                  <  1:188527/156‑1 (MQ=255)                                                                                                                                                                                                          attaAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGatat                                                                               <  1:201097/191‑1 (MQ=255)                                                                                                                                                                                                          attaAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGATATATCCTTCTTTCTATTGTTACTGTTATTCGCCTTTCTGTCACatat                                  <  1:593687/236‑1 (MQ=255)                                                                                                                                                                                                          attaAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGATATATCCTTCTTTCTATTGTTACTGTTATTCGCCTTTCTGTCACATATTGCCCTAGGc                        >  1:286126/1‑246 (MQ=255)                                                                                                                                                                                                          attaAAATTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGATATATCCTTCTTTCTATTGTTACTGTTATTCGCCTTTCTGTCACATATTGCCCTAGGCAtttt                   >  1:116561/1‑251 (MQ=255)                                                                                                                                                                                                               aaTTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGATATATCCTTCTTTCTATTGTTACTGTTATTCGCCTTTCTGTCACATATTGCCCTAGGCATTTTAGATa              >  1:614221/1‑251 (MQ=255)                                                                                                                                                                                                               aaTTAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGATATATCCTTCTTTCTATTGTTACTGTTATTCGCCTTTCTGTCACATATTGCCCTAGGCATTTTAGATa              >  1:871553/1‑251 (MQ=255)                                                                                                                                                                                                                  tAATGCCTCTAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGAc                                                                                                                                  >  1:315957/1‑132 (MQ=255)                                                                                                                                                                                                                           tAAATGCAAATAAAAAATCGTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGATATATCCTTCTTTCTATTGTTACTGTTATTCGCCTTTCTGTCACATATTGCCCTAGGCATTTTAGATAATTATGCCAATc  <  1:184261/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                        TGCGCTTACCACAACGGAACCGTCCGCATACACGCGTTGGCTTTCTTTTTCAATACCGAAAGAACCCTGCTGAAACAACAATTCCAAGCGATTTTCTTTTATAACCTGCTGAATTTTCATAAAACTTCCTTTAATCCGAGTTGAAAAACAAATTTACTTATTGCTACAGTCGGAATACATTTTTCAAAAAACAACCACATTATTAAAATTAATGCCTCTAAATGCAAATAAAAAATCTTAATTCTCATAAGGAACGGTGATAACCTCATGTATTGCTATGAAAATACTTCAATTTAAGTAACAAAGAGACTAAAATTTTGCCTTAATGAAAAACACATGACGGTAATAACAATGTTTCAATCAAAAAAATTCTCTTTTCTTTACAATGATATATCCTTCTTTCTATTGTTACTGTTATTCGCCTTTCTGTCACATATTGCCCTAGGCATTTTAGATAATTATGCCAATC  >  1/86325‑86793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑