Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	303,083	A→C	intergenic (+14/‑220)	unknown → / → unknown	AAA+ ATPase superfamily protein YifB/ComM, associated with DNA recombination/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	303,083	0	A	C	100.0%	172.4 / NA	61	intergenic (+14/‑220)	unknown/unknown	AAA+ ATPase superfamily protein YifB/ComM, associated with DNA recombination/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/36);  total (25/36)

GGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGCGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACTAGGAAGCCATTGAAAACGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGAAACAACGTCCTTTCCGTGCGCCCCATCACAGTGCTTCCACGCCGGCATTGGTCGGCGGCGGCTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAGGAAGTGGATTATGTCATATTTCAACGAAAAT  >  1/302844‑303323                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 ggACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAg                                                                                                                                                                                                                                       >  1:764884/1‑251 (MQ=255)  gACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAg                                                                                                                                                                                                                                       <  1:250724/250‑1 (MQ=255)   aCGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGc                                                                                                                                                                                                                                      <  1:483579/250‑1 (MQ=255)                  ccGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAATAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAAc                                                                                                                                                                                                                      <  1:519935/251‑1 (MQ=255)                               acaacaAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTAc                                                                                                                                                                                                                           >  1:834535/1‑233 (MQ=255)                                  acaaAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATa                                                                                                                                                                                                      >  1:879679/1‑251 (MQ=255)                                     aaGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACt                                                                                                                                                                                                   <  1:748215/251‑1 (MQ=255)                                               gATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCa                                                                                                                                                                                                        >  1:292844/1‑236 (MQ=255)                                                         aTGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataa                                                                                                                                                                               >  1:339274‑M1/1‑233 (MQ=255)                                                            aaaaTCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactg                                                                                                                                                                            <  1:343694‑M1/251‑22 (MQ=255)                                                                tCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaa                                                                                                                                                                        >  1:509281‑M1/1‑226 (MQ=255)                                                                            cACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                                                            <  1:258693‑M1/251‑38 (MQ=255)                                                                            cACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGGCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                                                            <  1:446605‑M1/251‑38 (MQ=255)                                                                              ccGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataat                                                                                                                                                          >  1:249772‑M1/1‑212 (MQ=255)                                                                                 atatTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattg                                                                                                                                                        <  1:415016‑M1/250‑42 (MQ=255)                                                                                 atatTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                                                            >  1:575320‑M1/1‑209 (MQ=255)                                                                                  tattatCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgt                                                                                                                                                       >  1:74948‑M1/1‑208 (MQ=255)                                                                                  tattatCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGAACAGGAAGCCATTGAAACCGCGTCCTTAGCAATTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaa                                                                                                                                                                                         <  1:581270‑M1/216‑9 (MQ=255)                                                                                         ggTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttat                                                                                                                                               >  1:321165‑M1/1‑201 (MQ=255)                                                                                          gTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAAc                                                                                                                                                                                                                      <  1:319035/179‑1 (MQ=255)                                                                                          gTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttata                                                                                                                                              >  1:782112‑M1/1‑200 (MQ=255)                                                                                          gTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataac                                                                                                                                                                              >  1:78195‑M1/1‑200 (MQ=255)                                                                                             aacaacACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGCACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacc                                                                                                                                           <  1:309830‑M1/251‑55 (MQ=255)                                                                                              acaacaCGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataaccc                                                                                                                                          <  1:687227‑M1/251‑56 (MQ=255)                                                                                                         aaCGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                                                            <  1:635910‑M1/222‑38 (MQ=255)                                                                                                           cgtgctctCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataa                                                                                                                             <  1:540610‑M1/248‑69 (MQ=255)                                                                                                            gtgctcTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataat                                                                                                                            >  1:633112‑M1/3‑182 (MQ=255)                                                                                                              gcTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataac                                                                                                                                                                              <  1:140868‑M1/199‑20 (MQ=255)                                                                                                                tctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctac                                                                                                                        <  1:58569‑M1/251‑74 (MQ=255)                                                                                                                 ctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTg                                                                                                                                                                                                                >  1:228485/1‑162 (MQ=255)                                                                                                                 ctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatcta                                                                                                                         >  1:475741‑M1/1‑177 (MQ=255)                                                                                                                 ctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                                                            >  1:483495‑M1/1‑177 (MQ=255)                                                                                                                   tggttttcccggtgccgggcaacacaatctGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                                                            <  1:323011‑M1/199‑38 (MQ=255)                                                                                                                        tCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctat                                                                                                                >  1:820765‑M1/1‑170 (MQ=255)                                                                                                                           cAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctattt                                                                                                              <  1:695226‑M1/250‑84 (MQ=255)                                                                                                                              ccgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaag                                                                                                           <  1:316137‑M1/250‑87 (MQ=255)                                                                                                                              ccgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaag                                                                                                           <  1:574467‑M1/250‑87 (MQ=255)                                                                                                                               cgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtg                                                                                                         <  1:882266‑M1/251‑89 (MQ=255)                                                                                                                                   gggCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataaccc                                                                                                                                          <  1:717521‑M1/214‑56 (MQ=255)                                                                                                                                       aaCACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaa                                                                                                 <  1:140829‑M1/251‑97 (MQ=255)                                                                                                                                        acacAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaag                                                                                                >  1:667043‑M1/1‑154 (MQ=255)                                                                                                                                        acacAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaag                                                                                                <  1:157212‑M1/251‑98 (MQ=255)                                                                                                                                           caATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatc                                                                                                                           <  1:506082‑M1/221‑71 (MQ=255)                                                                                                                                             aTCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataaccc                                                                                                                                          <  1:422369‑M1/204‑56 (MQ=255)                                                                                                                                               cTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataa                                                                                                                             >  1:17850‑M1/1‑147 (MQ=255)                                                                                                                                               cTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacga                                                                                                                                                                    <  1:614147‑M1/176‑30 (MQ=255)                                                                                                                                                 gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGtt                                                                                                                                                                                                                                 <  1:688122/113‑1 (MQ=255)                                                                                                                                                 gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagt                                                                                                          <  1:548754‑M1/232‑88 (MQ=255)                                                                                                                                                 gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaag                                                                                                           <  1:521285‑M1/231‑87 (MQ=255)                                                                                                                                                 gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattg                                                                                                                                                        >  1:154771‑M1/1‑145 (MQ=255)                                                                                                                                                      ttCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataaccc                                                                                                                                          <  1:284527‑M1/195‑56 (MQ=255)                                                                                                                                                            gTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgtcatat                                                                             <  1:301906‑M1/250‑117 (MQ=255)                                                                                                                                                                gCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatc                                                                                                                           >  1:695205‑M1/1‑130 (MQ=255)                                                                                                                                                                          gggAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgtcatatttcaacgaaaataaa                                                              >  1:114659‑M1/1‑120 (MQ=255)                                                                                                                                                                                      cTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactg                                                                                                                                                                            <  1:474465‑M1/129‑22 (MQ=255)                                                                                                                                                                                              ccGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgtcatatttcaacgaaaataaattagagaaagttcgctgg                                            <  1:855294‑M1/249‑150 (MQ=255)                                                                                                                                                                                              ccGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacc                                                                                                                   <  1:311556‑M1/178‑79 (MQ=255)                                                                                                                                                                                                gTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAGACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtg                                                                                                         >  1:543755‑M1/1‑98 (MQ=255)                                                                                                                                                                                                     aCCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgtcatatttcaacgaaaa                                                                  >  1:698679‑M1/1‑93 (MQ=255)                                                                                                                                                                                                                                      gCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgtcatatttcaacgaaaataaattagagaaagttcgctggcgttgttcgcttaatttagaaaagtattttgaaaaacctctt  >  1:508062‑M1/1‑60 (MQ=255)                                                                                                                                                                                                                                        cATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgt                                                                                  <  1:834787‑M1/169‑112 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 GGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGCGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACTAGGAAGCCATTGAAAACGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGAAACAACGTCCTTTCCGTGCGCCCCATCACAGTGCTTCCACGCCGGCATTGGTCGGCGGCGGCTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAGGAAGTGGATTATGTCATATTTCAACGAAAAT  >  1/302844‑303323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑