Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	303,067	T→C	*245Q (TAG→CAG)	unknown →	AAA+ ATPase superfamily protein YifB/ComM, associated with DNA recombination

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	303,067	0	T	C	100.0%	196.8 / NA	65	*245Q (TAG→CAG)	unknown	AAA+ ATPase superfamily protein YifB/ComM, associated with DNA recombination
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/38);  total (27/38)

GTAGTGCAATTTCTCAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGCGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACTAGGAAGCCATTGAAAACGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGAAACAACGTCCTTTCCGTGCGCCCCATCACAGTGCTTCCACGCCGGCATTGGTCGGCGGCGGCTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAG  >  1/302821‑303281                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                       gtagtGCAATTTCTCAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGa                                                                                                                                                                                                                    >  1:234381/1‑251 (MQ=255)           ttCTCAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGaa                                                                                                                                                                                                          <  1:420133/251‑1 (MQ=255)            tctcAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAACATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACAGGAAATGACCGACCAGGAAGCCATTGaaa                                                                                                                                                                                                         >  1:300215/1‑251 (MQ=255)                        ggACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAg                                                                                                                                                                                             >  1:764884/1‑251 (MQ=255)                         gACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAg                                                                                                                                                                                             <  1:250724/250‑1 (MQ=255)                          aCGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGc                                                                                                                                                                                            <  1:483579/250‑1 (MQ=255)                                         ccGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAATAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAAc                                                                                                                                                                            <  1:519935/251‑1 (MQ=255)                                                      acaacaAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTAc                                                                                                                                                                                 >  1:834535/1‑233 (MQ=255)                                                         acaaAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATa                                                                                                                                                            >  1:879679/1‑251 (MQ=255)                                                            aaGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACt                                                                                                                                                         <  1:748215/251‑1 (MQ=255)                                                                      gATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCa                                                                                                                                                              >  1:292844/1‑236 (MQ=255)                                                                                aTGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataa                                                                                                                                     >  1:339274‑M1/1‑233 (MQ=255)                                                                                   aaaaTCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactg                                                                                                                                  <  1:343694‑M1/251‑22 (MQ=255)                                                                                       tCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaa                                                                                                                              >  1:509281‑M1/1‑226 (MQ=255)                                                                                                   cACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGaa                                                                                                                                                                                                          <  1:767968/163‑1 (MQ=255)                                                                                                   cACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                  <  1:258693‑M1/251‑38 (MQ=255)                                                                                                   cACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGGCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                  <  1:446605‑M1/251‑38 (MQ=255)                                                                                                     ccGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataat                                                                                                                >  1:249772‑M1/1‑212 (MQ=255)                                                                                                        atatTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattg                                                                                                              <  1:415016‑M1/250‑42 (MQ=255)                                                                                                        atatTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                  >  1:575320‑M1/1‑209 (MQ=255)                                                                                                         tattatCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgt                                                                                                             >  1:74948‑M1/1‑208 (MQ=255)                                                                                                         tattatCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGAACAGGAAGCCATTGAAACCGCGTCCTTAGCAATTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaa                                                                                                                                               <  1:581270‑M1/216‑9 (MQ=255)                                                                                                                ggTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttat                                                                                                     >  1:321165‑M1/1‑201 (MQ=255)                                                                                                                 gTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAAc                                                                                                                                                                            <  1:319035/179‑1 (MQ=255)                                                                                                                 gTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttata                                                                                                    >  1:782112‑M1/1‑200 (MQ=255)                                                                                                                 gTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataac                                                                                                                                    >  1:78195‑M1/1‑200 (MQ=255)                                                                                                                    aacaacACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGCACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacc                                                                                                 <  1:309830‑M1/251‑55 (MQ=255)                                                                                                                     acaacaCGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataaccc                                                                                                <  1:687227‑M1/251‑56 (MQ=255)                                                                                                                           cGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGaaac                                                                                                                                                                                                        >  1:593567/1‑140 (MQ=255)                                                                                                                                aaCGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                  <  1:635910‑M1/222‑38 (MQ=255)                                                                                                                                  cgtgctctCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataa                                                                                   <  1:540610‑M1/248‑69 (MQ=255)                                                                                                                                   gtgctcTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataat                                                                                  >  1:633112‑M1/3‑182 (MQ=255)                                                                                                                                     gcTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataac                                                                                                                                    <  1:140868‑M1/199‑20 (MQ=255)                                                                                                                                       tctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctac                                                                              <  1:58569‑M1/251‑74 (MQ=255)                                                                                                                                        ctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTg                                                                                                                                                                      >  1:228485/1‑162 (MQ=255)                                                                                                                                        ctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatcta                                                                               >  1:475741‑M1/1‑177 (MQ=255)                                                                                                                                        ctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                  >  1:483495‑M1/1‑177 (MQ=255)                                                                                                                                          tggttttcccggtgccgggcaacacaatctGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaata                                                                                                                  <  1:323011‑M1/199‑38 (MQ=255)                                                                                                                                               tCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctat                                                                      >  1:820765‑M1/1‑170 (MQ=255)                                                                                                                                                  cAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctattt                                                                    <  1:695226‑M1/250‑84 (MQ=255)                                                                                                                                                     ccgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaag                                                                 <  1:316137‑M1/250‑87 (MQ=255)                                                                                                                                                     ccgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaag                                                                 <  1:574467‑M1/250‑87 (MQ=255)                                                                                                                                                      cgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtg                                                               <  1:882266‑M1/251‑89 (MQ=255)                                                                                                                                                          gggCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataaccc                                                                                                <  1:717521‑M1/214‑56 (MQ=255)                                                                                                                                                              aaCACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaa                                                       <  1:140829‑M1/251‑97 (MQ=255)                                                                                                                                                               acacAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaag                                                      >  1:667043‑M1/1‑154 (MQ=255)                                                                                                                                                               acacAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaag                                                      <  1:157212‑M1/251‑98 (MQ=255)                                                                                                                                                                  caATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatc                                                                                 <  1:506082‑M1/221‑71 (MQ=255)                                                                                                                                                                    aTCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataaccc                                                                                                <  1:422369‑M1/204‑56 (MQ=255)                                                                                                                                                                      cTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataa                                                                                   >  1:17850‑M1/1‑147 (MQ=255)                                                                                                                                                                      cTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacga                                                                                                                          <  1:614147‑M1/176‑30 (MQ=255)                                                                                                                                                                        gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGtt                                                                                                                                                                                       <  1:688122/113‑1 (MQ=255)                                                                                                                                                                        gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagt                                                                <  1:548754‑M1/232‑88 (MQ=255)                                                                                                                                                                        gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaag                                                                 <  1:521285‑M1/231‑87 (MQ=255)                                                                                                                                                                        gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattg                                                                                                              >  1:154771‑M1/1‑145 (MQ=255)                                                                                                                                                                             ttCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataaccc                                                                                                <  1:284527‑M1/195‑56 (MQ=255)                                                                                                                                                                                   gTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgtcatat                                   <  1:301906‑M1/250‑117 (MQ=255)                                                                                                                                                                                       gCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGaaac                                                                                                                                                                                                        <  1:248888/81‑2 (MQ=255)                                                                                                                                                                                       gCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatc                                                                                 >  1:695205‑M1/1‑130 (MQ=255)                                                                                                                                                                                                 gggAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgtcatatttcaacgaaaataaa                    >  1:114659‑M1/1‑120 (MQ=255)                                                                                                                                                                                                             cTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactg                                                                                                                                  <  1:474465‑M1/129‑22 (MQ=255)                                                                                                                                                                                                                     ccGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgtcatatttcaacgaaaataaattagagaaagttcgctgg  <  1:855294‑M1/249‑150 (MQ=255)                                                                                                                                                                                                                     ccGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacc                                                                         <  1:311556‑M1/178‑79 (MQ=255)                                                                                                                                                                                                                       gTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAGACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtg                                                               >  1:543755‑M1/1‑98 (MQ=255)                                                                                                                                                                                                                            aCCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGtgttaaaattacaaataactgtcaaacgatacaaataattgtcgccttataacccaaatcatttataatctacttacctatttaagtggctgaaaaggaagtggattatgtcatatttcaacgaaaa                        >  1:698679‑M1/1‑93 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                       GTAGTGCAATTTCTCAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGCGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACTAGGAAGCCATTGAAAACGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGAAACAACGTCCTTTCCGTGCGCCCCATCACAGTGCTTCCACGCCGGCATTGGTCGGCGGCGGCTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAG  >  1/302821‑303281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑