| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 223,012 | C→G | A111A (GCG→GCC) | unknown ← | Nitrogen regulatory protein P‑II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 223,012 | 0 | C | G | 98.9% | 251.1 / NA | 88 | A111A (GCG→GCC) | unknown | Nitrogen regulatory protein P‑II |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (36/51); minor base T (1/0); total (37/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CCGGTTGCTGCACAAACTGTTGCAACTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATCGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTATGCCCTTTTTGCCGCCCGAAACCGCGCACCTCAGTCAC > 1/222768‑223257 | ccGGTTGCTGCACAAACTGTTGCAACTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTTAAGAAAACGCGCCTTGTCGAAAAAAGGCTCGGTGTTAT‑CGGTATTATTTTCCCTGGATTAAATGGCATc > 1:598594/1‑250 (MQ=255)ccGGTTGCTGCACAAACTGTTGCAACTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAAACGTGCTTTAAAGAAAACGCGCCCTGTCGAAAAAAGGCTCGGGGTTAT‑CGGGATTATTTCCCATGGATTAAATGGCATc > 1:771648/1‑250 (MQ=255) ttGCTGCACAAACTGTTGCAACTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAACATAAAAACGTGCTTTTAAAAAAAAGCGCCTTGTCGAAAAAAAGCTCGGTGGTTT‑TGGGATTATTTTCCATGGATTAAAAGGCATCCTCAt > 1:866828/1‑251 (MQ=255) ctgcACAAACTGTTGCAACTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTTAAGAAAACGCGCCTTGTCGAAAAAAGGCTCGGGGTTAT‑CGGGATTATTTCCCATGGATTTAATGGCATCCTCAtttt > 1:188444/1‑251 (MQ=255) tgcACAAACTGTTGCAACTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTttc < 1:826695/251‑1 (MQ=255) tgcACAAACTGTTGCAACTGCAGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTttc < 1:464771/251‑1 (MQ=255) gcACAAACTGTTGCAACTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCAtttt > 1:688271/1‑249 (MQ=255) aaCTGTTGCAACTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGcc < 1:381178/251‑1 (MQ=255) aCTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATa < 1:398007/251‑1 (MQ=255) cTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGGGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGGGTTAT‑CGGGATTATTTCCCATGGATTAAATGGCATCCTCATTTTTTTCGCCCGTGCGG‑ATa > 1:110263/1‑250 (MQ=255) cGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATGGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGGATTATTTCCCATGGATTAAATGGCATCCTCCTTTTTTTCGCCCGTGCGG‑ATACGAAt > 1:810126/1‑251 (MQ=255) cccAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGGGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGt > 1:413895/1‑251 (MQ=255) aaTCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCAc < 1:677774/251‑1 (MQ=255) aaTCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCAc < 1:706294/251‑1 (MQ=255) tCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCt < 1:469938/251‑1 (MQ=255) aagaagAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGCGCTTTAAAGAAAAAGCGCCTTGTCGAAACAAGGCTCGGGGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGAGCGG‑ATACGAATCACCCGTTCCACCTCGTACACaaaaa > 1:730787/1‑251 (MQ=255) aagaaCAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑Tc < 1:203700/250‑1 (MQ=255) ttCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGcagcaaccgg < 1:871614/251‑10 (MQ=255) tCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCaata < 1:526769/251‑1 (MQ=255) tCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCaata < 1:466928/251‑1 (MQ=255) tCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCaata < 1:127160/251‑1 (MQ=255) aaGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATgg < 1:568075/251‑1 (MQ=255) aaGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATgg < 1:42479/251‑1 (MQ=255) gCGTTATCGTAAGATGATGAAATTCTCTACGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAAACGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGGGTTAT‑CGGGATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCg > 1:667954/1‑251 (MQ=255) aTCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTTTAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGGATTATTTTCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCCTCACCGATTTTGCCCGTTTGTGCTGTATCAATAAAGGCGTCCaa > 1:666589/1‑251 (MQ=255) aTCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGcc < 1:136796/158‑1 (MQ=255) aTCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGGGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTTTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTTCCATCACCGATTTTGCCCGTTTGTGCTGTATCaataa > 1:152502/1‑241 (MQ=255) aTCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGGGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCaa > 1:884067/1‑251 (MQ=255) aTCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAAAAAGGCTCGGTGTTAT‑CGGGATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTAt > 1:287178/1‑235 (MQ=255) cGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGc < 1:478595/251‑1 (MQ=255) atgatgAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGt < 1:120590/251‑1 (MQ=255) atgaAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCa < 1:246084/136‑1 (MQ=255) tttctATGGAAATTATGACAAAAAGTGGCGGGATTATAGAATAAAACCGGGCTTTAAAGCAAACGCGCCTTTACGAAAAAATGCCAGGAGTTATACGGT‑TTATTTCCCCTGGTCTAAATTGCATTCTCATTTTCTTTCCCCCTGGGGAATA‑GAATCACCCGGTCCACCCAGGACACAAAAATTTTTTCCA‑CAGCGATTTTGTCCGATTGTGTTGTTTCTATAATGGCggcccatcactggttttaaaaata > 1:802956/3‑228 (MQ=255) tctcTATGGAAATTATGACAAAAAGTGGCGGGGTTATAGCATAAAAACGGGCTTTAAAGAAAACGCGCCTTGTCGAAAAAAGGCTCGGGGTTAT‑CGGTATTATTTCCCCTGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTTCCCGTTTGTGCTGTATCAATAATGGGGTCCAAGCATCGGTCTAACAAAt > 1:802945/1‑250 (MQ=255) tctcTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGGATTATTTCCCATGGGTTAAATGGCATCCTCCTTTTCTTCGCCCGTGCGG‑GTACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTTCCCGTTTGTGc > 1:584981/1‑212 (MQ=255) aTGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAAACGTGCTTTAAAGAAAAAGCGCCTTGTCGAAACAAGGCTCGGGGTTAT‑CGGGATTATTTCCCATGGATTAAATGGCATCCTCCTTTTTTTTGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTgg > 1:710398/1‑251 (MQ=255) gACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAAt < 1:362565/139‑1 (MQ=255) tGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGGCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCAttt < 1:518167/249‑1 (MQ=255) cGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCAc < 1:425337/250‑1 (MQ=255) cGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCAc < 1:337295/250‑1 (MQ=255) ggATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCAtt > 1:616630/1‑101 (MQ=255) ggATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATa < 1:56660/120‑1 (MQ=255) ggATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCaata < 1:268935/192‑1 (MQ=255) ggATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCAt < 1:763210/207‑1 (MQ=255) ggATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACttt > 1:217540/1‑251 (MQ=255) gATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTgg > 1:347695/1‑225 (MQ=255) aTTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCaata < 1:246044/190‑1 (MQ=255) ttATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCaataat < 1:893029/191‑1 (MQ=255) gCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATAc < 1:326389/113‑1 (MQ=255) gCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGc > 1:83728/1‑220 (MQ=255) gCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCCCTTTCGgaagaa > 1:757674/1‑251 (MQ=255) gCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGaa > 1:362803/1‑251 (MQ=255) cATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCaataa < 1:691077/184‑1 (MQ=255) aCCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATcca > 1:25446/1‑250 (MQ=255) gCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATgg > 1:819136/1‑176 (MQ=255) gCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAg < 1:227473/237‑1 (MQ=255) cTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCatat < 1:729639/250‑1 (MQ=255) tttAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCatatat < 1:547783/251‑1 (MQ=255) tttAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCatatat < 1:336575/251‑1 (MQ=255) ttAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATAtt < 1:588581/251‑1 (MQ=255) ttAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATAtt < 1:802193/251‑1 (MQ=255) aaaGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCaataa < 1:729243/168‑1 (MQ=255) aaaGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCg > 1:662745/1‑251 (MQ=255) gTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCaata > 1:666032/1‑151 (MQ=255) tCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTc < 1:671160/251‑1 (MQ=255) tCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTc < 1:231045/251‑1 (MQ=255) tCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTc < 1:63042/251‑1 (MQ=255) aCAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTAt < 1:323609/251‑1 (MQ=255) cGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACaa < 1:333258/93‑1 (MQ=255) cGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTATGCCCtttt < 1:131678/251‑1 (MQ=255) cGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTCTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTATGCCCtttt > 1:172986/1‑251 (MQ=255) gtgtTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTAt > 1:368671/1‑130 (MQ=255) gtgtTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATACATTCGGCACCGCGATACAATTCCGTATGCCCTTTTTg > 1:190507/1‑251 (MQ=255) tgtTAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCTATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTATGCCCTTTTTgc < 1:67201/251‑1 (MQ=255) tAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGc > 1:657495/1‑167 (MQ=255) tAT‑CGGTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTATGCCCTTTTTgccg > 1:769193/1‑250 (MQ=255) gTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAAt > 1:191453/1‑59 (MQ=255) gTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACaa < 1:439360/152‑1 (MQ=255) gTATTATTTCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCa > 1:790917/1‑156 (MQ=255) ttCCCATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTATGCCCTTTTTGCCGCCCGAAACCgcgc < 1:715587/251‑1 (MQ=255) cccATGGATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATAtt > 1:615043/1‑199 (MQ=255) ggATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCa < 1:140089/161‑1 (MQ=255) ggATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATAtt < 1:231722/194‑1 (MQ=255) ggATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGc < 1:791264/198‑1 (MQ=255) ggATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTATGCCCTTTTTGCCGCCCGAAACCGCGCACCTCAg > 1:411336/1‑251 (MQ=255) gATTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGc < 1:285395/101‑1 (MQ=255) aTTAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCAtt < 1:591103/161‑1 (MQ=255) tAAATGGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTATGCCCTTTTTGCCGCCCGAAACCGCGCACCTCAGTCAc < 1:807520/251‑1 (MQ=255) | CCGGTTGCTGCACAAACTGTTGCAACTGCCGGTATCCCCAAAATCCTGCGCCGGCAAGAAGAACAAGTAAAAACAAAAGAAATAAACAAAATTTTTTCATAAGCGTTATCGTAAGATGATGAAATTCTCTATGGAAATTATGACAAAAAGTGGCGGGATTATAGCATAAAACCGTGCTTTAAAGAAAACGCGCCTTGTCGAAACAAGGCTCGGTGTTAT‑CGGTATTATTTCCCATGGATTAAATCGCATCCTCATTTTCTTCGCCCGTGCGG‑ATACGAATCACCCGTTCCACCTCGTACACAAAAA‑TCTTGCCATCACCGATTTTGCCCGTTTGTGCTGTATCAATAATGGCGTCCAAGCATCGGTCTAACAAATCATTGGGCACCACAACTTCCATTTTCACTTTCGGTAGAAAATCCACCATATATTCGGCACCGCGATACAATTCCGTATGCCCTTTTTGCCGCCCGAAACCGCGCACCTCAGTCAC > 1/222768‑223257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |