Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 74,205 A→G T93A (ACC→GCC)  unknown → Type IV pilus biogenesis protein PilF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*174,2050AG100.0% 224.5 / NA 78T93A (ACC→GCC) unknownType IV pilus biogenesis protein PilF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/33);  total (45/33)

TATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGTACCACCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGA  >  1/73967‑74445
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
tATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAAcg                                                                                                                                                                                                                                       <  1:18247/251‑1 (MQ=255)
tATTTTACCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGAGTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAAcg                                                                                                                                                                                                                                       <  1:610775/251‑1 (MQ=255)
          tttttGCTTTCCGCCTGCGTCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAAt                                                                                                                                                                                                                              <  1:610219/250‑1 (MQ=255)
                cTTTCCGCCTGCGTCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAgatga                                                                                                                                                                                                                       >  1:88390/1‑251 (MQ=255)
                            gTCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTAtt                                                                                                                                                                                                                                             <  1:69147/217‑1 (MQ=255)
                             tCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAAcg                                                                                                                                                                                                                                       >  1:22910/1‑222 (MQ=255)
                             tCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGa                                                                                                                                                                                                          <  1:893735/250‑1 (MQ=255)
                             tCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGa                                                                                                                                                                                                          <  1:326324/250‑1 (MQ=255)
                               aCCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAAcg                                                                                                                                                                                                                                       <  1:160431/220‑1 (MQ=255)
                               aCCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑ggg                                                                                                                                                                                                            <  1:368929/246‑1 (MQ=255)
                               aCCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGAtgt                                                                                                                                                                                                       >  1:108339/1‑251 (MQ=255)
                                cccAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGAtgtg                                                                                                                                                                                                      <  1:236387/251‑1 (MQ=255)
                                    acaaAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCa                                                                                                                                                                                                  >  1:461444/1‑251 (MQ=255)
                                      aaaGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCaat                                                                                                                                                                                                <  1:237044/251‑1 (MQ=255)
                                          tGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCaataa                                                                                                                                                                                              <  1:738922/249‑1 (MQ=255)
                                          tGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCaataa                                                                                                                                                                                              <  1:562163/249‑1 (MQ=255)
                                               tGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACgg                                                                                                                                                                                        <  1:620216/250‑1 (MQ=255)
                                               tGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACgg                                                                                                                                                                                        <  1:396423/250‑1 (MQ=255)
                                                      tGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTc                                                                                                                                                                                <  1:387149/251‑1 (MQ=255)
                                                            gCAAAGAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTtgtg                                                                                                                                                                          >  1:610483/1‑251 (MQ=255)
                                                                     cgccAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAgggg                                                                                                                                                                 <  1:887132/251‑1 (MQ=255)
                                                                          aaGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAAtt                                                                                                                                                             >  1:780497/1‑250 (MQ=255)
                                                                               cgcgTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTa                                                                                                                                                                                                                                           <  1:739601/168‑1 (MQ=255)
                                                                                     gAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTa                                                                                                                                                 >  1:190975/1‑251 (MQ=255)
                                                                                       aCTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATg                                                                                                                                               <  1:521276/251‑1 (MQ=255)
                                                                                                   cTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGTCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGaa                                                                                                                                    <  1:178533/250‑1 (MQ=255)
                                                                                                   cTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGaa                                                                                                                                    <  1:352320/250‑1 (MQ=255)
                                                                                                     aTTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAAcg                                                                                                                                  >  1:376073/1‑250 (MQ=255)
                                                                                                     aTTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAAcg                                                                                                                                  >  1:869927/1‑250 (MQ=255)
                                                                                                                        ggCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCtt                                                                                                                                                                              <  1:888347/187‑1 (MQ=255)
                                                                                                                         gCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATg                                                                                                                                               <  1:337082/217‑1 (MQ=255)
                                                                                                                             aagccaagcTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTAc                                                                                                         <  1:860980/251‑1 (MQ=255)
                                                                                                                              agccaagcTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑g                                                                                                                                                                                                              >  1:821517/1‑149 (MQ=255)
                                                                                                                              agccaagcTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTAc                                                                                                         >  1:8051/1‑250 (MQ=255)
                                                                                                                              agccaagcTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTAc                                                                                                         >  1:558820/1‑250 (MQ=255)
                                                                                                                                   agcTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑g                                                                                                                                                                                                              <  1:464176/144‑1 (MQ=255)
                                                                                                                                        aaCCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTc                                                                                                                                                                                <  1:493796/169‑1 (MQ=255)
                                                                                                                                         aCCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTAc                                                                                                                                            >  1:221204/1‑204 (MQ=255)
                                                                                                                                         aCCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGac                                                                                             <  1:824319/251‑1 (MQ=255)
                                                                                                                                             ggATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTAc                                                                                                                                            >  1:818497/1‑200 (MQ=255)
                                                                                                                                              gATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTa                                                                                        >  1:873744/1‑251 (MQ=255)
                                                                                                                                                    gCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGaaaa                                                                                  >  1:861740/1‑251 (MQ=255)
                                                                                                                                                                 cTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGaa                                                                                                                                                               >  1:877776/1‑161 (MQ=255)
                                                                                                                                                                   cccGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAgat                                                                                                                                                                                                                         >  1:52215/1‑102 (MQ=255)
                                                                                                                                                                   cccGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTaaaa                                                                                                                        >  1:666184/1‑198 (MQ=255)
                                                                                                                                                                   cccGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTAc                                                                                                         >  1:204059/1‑213 (MQ=255)
                                                                                                                                                                   cccGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGaaa                                                                                   <  1:42472/235‑1 (MQ=255)
                                                                                                                                                                   cccGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGc                                                                   <  1:779163/251‑1 (MQ=255)
                                                                                                                                                                        ttattattTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCaataa                                                                                                                                                                                              >  1:898133/1‑123 (MQ=255)
                                                                                                                                                                        ttattattTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAg                                                                                                                                                       >  1:599321/1‑162 (MQ=255)
                                                                                                                                                                        ttattattTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTAt                                                              >  1:162861/1‑251 (MQ=255)
                                                                                                                                                                          attattTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATg                                                                                      >  1:155719/1‑225 (MQ=255)
                                                                                                                                                                          attattTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATcc                                                            <  1:514132/251‑1 (MQ=255)
                                                                                                                                                                          attattTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATc                                                             >  1:381722/1‑250 (MQ=255)
                                                                                                                                                                             attTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTAc                                                                                                                                            <  1:157872/168‑1 (MQ=255)
                                                                                                                                                                                  gtgtCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATc                                                             >  1:282094/1‑242 (MQ=255)
                                                                                                                                                                                    gtCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTAc                                                                                                                                            >  1:324569/1‑161 (MQ=255)
                                                                                                                                                                                      cAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTAc                                                                                                                                            >  1:679072/1‑159 (MQ=255)
                                                                                                                                                                                                 gCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCg                                     >  1:629356/1‑251 (MQ=255)
                                                                                                                                                                                                        tCTATCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAAc                                                                                 >  1:624071/1‑200 (MQ=255)
                                                                                                                                                                                                           aTCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAAt                              >  1:401594/1‑249 (MQ=255)
                                                                                                                                                                                                           aTCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCtt                                                                                                                                                                              >  1:575279/1‑104 (MQ=255)
                                                                                                                                                                                                           aTCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGaaa                                                                                   >  1:598209/1‑195 (MQ=255)
                                                                                                                                                                                                           aTCAACAACAGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAAtct                            >  1:141631/1‑250 (MQ=255)
                                                                                                                                                                                                               acaacaGGGCGACATTGAACAAGCCCGCACCGCCTATTTGAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCat                       <  1:336606/251‑1 (MQ=255)
                                                                                                                                                                                                                caacaGGGCGACATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCatt                      >  1:27176/1‑251 (MQ=255)
                                                                                                                                                                                                                          aCATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGaaa            >  1:53610/1‑251 (MQ=255)
                                                                                                                                                                                                                           cATTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑TGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCa                                                                                                       >  1:581446/1‑159 (MQ=255)
                                                                                                                                                                                                                            aTTGAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAAt          <  1:27881/251‑1 (MQ=255)
                                                                                                                                                                                                                               gAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGaaa                                                                                   >  1:285897/1‑175 (MQ=255)
                                                                                                                                                                                                                               gAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCg                                                                           <  1:501496/183‑1 (MQ=255)
                                                                                                                                                                                                                               gAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAAtct        >  1:842013/1‑250 (MQ=255)
                                                                                                                                                                                                                               gAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAAtct        >  1:200891/1‑250 (MQ=255)
                                                                                                                                                                                                                               gAACAAGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAAtct        >  1:701632/1‑250 (MQ=255)
                                                                                                                                                                                                                                    aGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAAtc         >  1:295642/1‑244 (MQ=255)
                                                                                                                                                                                                                                    aGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGa  >  1:859367/1‑251 (MQ=255)
                                                                                                                                                                                                                                    aGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGa  >  1:50579/1‑251 (MQ=255)
                                                                                                                                                                                                                                    aGCCCGCACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGa  >  1:116800/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
TATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAGTGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGGCACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTCTATCAACAACAGGGCGACATTGAACAAGCCCGTACCACCTATTTAAACGCGATAAAATTAGATGATAAACAA‑GGGGATGTGTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAAGCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTATCGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGA  >  1/73967‑74445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑