BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	168,742	A→C	I406I (ATA→ATC)	unknown →	NADP‑specific glutamate dehydrogenase (EC 1.4.1.4)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	168,742	0	A	C	100.0%	338.0 / NA	112	I406I (ATA→ATC)	unknown	NADP‑specific glutamate dehydrogenase (EC 1.4.1.4)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (43/69); total (43/69)

Only 100 of 112 total aligned reads displayed.

CCAAAACGAATTGGCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATAATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGACGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACATTATTT  >  1/168496‑168983

ccAAAACGAATTGGCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGc                                                                                                                                                                                                                                                <  1:557603/251‑1 (MQ=255)

     aCGAATTGGCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGat                                                                                                                                                                                                                                           <  1:697764/251‑1 (MQ=255)

     aCGAATTGGCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGat                                                                                                                                                                                                                                           <  1:188277/251‑1 (MQ=255)

       gAATTGGCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGatat                                                                                                                                                                                                                                         >  1:211474/1‑251 (MQ=255)

         aTTGGCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTc                                                                                                                                                                                                                                       <  1:369669/251‑1 (MQ=255)

          ttGGCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCa                                                                                                                                                                                                                                      <  1:489531/251‑1 (MQ=255)

           tGGCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCAc                                                                                                                                                                                                                                     <  1:791686/251‑1 (MQ=255)

             gCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACg                                                                                                                                                                                                                                    >  1:522815/1‑250 (MQ=255)

                          cgcAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑aaaaaa                                                                                                                                                                                                                     <  1:264456/251‑1 (MQ=255)

                          cgcAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑aaaaaa                                                                                                                                                                                                                     >  1:797701/1‑251 (MQ=255)

                          cgcAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑aaaaaa                                                                                                                                                                                                                     <  1:363865/251‑1 (MQ=255)

                          cgcAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑aaaaa                                                                                                                                                                                                                      <  1:553127/250‑1 (MQ=255)

                             aaaCCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATAc                                                                                                                                                                                                                  <  1:845037/251‑1 (MQ=255)

                              aaCCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACg                                                                                                                                                                                                                 >  1:837921/1‑251 (MQ=255)

                              aaCCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACg                                                                                                                                                                                                                 >  1:126243/1‑251 (MQ=255)

                                cccTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑aaaaa                                                                                                                                                                                                                      >  1:606798/1‑244 (MQ=255)

                                cccTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACgg                                                                                                                                                                                                                >  1:349024/1‑250 (MQ=255)

                                cccTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGc                                                                                                                                                                                                               <  1:227239/251‑1 (MQ=255)

                                  cTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCt                                                                                                                                                                                                              >  1:525552/1‑250 (MQ=255)

                                       cGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACg                                                                                                                                                                                                        >  1:433929/1‑251 (MQ=255)

                                               acgTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑aaaaa                                                                                                                                                                                                                      >  1:400489/1‑229 (MQ=255)

                                               acgTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAgg                                                                                                                                                                                                 >  1:27621/1‑250 (MQ=255)

                                                        tggtggCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAAc                                                                                                                                                                                       <  1:581164/251‑1 (MQ=255)

                                                        tggtggCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAAc                                                                                                                                                                                       <  1:901252/251‑1 (MQ=255)

                                                         ggtggCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACAt                                                                                                                                                                                           <  1:38316/246‑1 (MQ=255)

                                                           tggCGGAAGGCGCCAATATGCCAACCACCATCGTCGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTa                                                                                                                                                                                     <  1:334144/250‑1 (MQ=255)

                                                           tggCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTa                                                                                                                                                                                     <  1:666312/250‑1 (MQ=255)

                                                           tggCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTa                                                                                                                                                                                     <  1:403508/250‑1 (MQ=255)

                                                                gAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATgtggg                                                                                                                                                                               >  1:32771/1‑250 (MQ=255)

                                                                    gcgcCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGtggtgg                                                                                                                                                                             <  1:363832/249‑1 (MQ=255)

                                                                       ccAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCa                                                                                                                                                                                                                                      <  1:130802/190‑1 (MQ=255)

                                                                              gCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCg                                                                                                                                                                 >  1:667081/1‑251 (MQ=255)

                                                                                  accaccATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGc                                                                                                                                                                                                                                   >  1:114846/1‑182 (MQ=255)

                                                                                  accaccATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCgg                                                                                                                                                             <  1:161495/251‑1 (MQ=255)

                                                                                           gACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGc                                                                                                                                                                                                >  1:496183/1‑207 (MQ=255)

                                                                                            aCGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGaaa                                                                                                                                                   >  1:564716/1‑251 (MQ=255)

                                                                                                  ccaATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGc                                                                                                                                              >  1:709034/1‑250 (MQ=255)

                                                                                                                       gCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAATGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAgg                                                                                                                         <  1:452305/250‑1 (MQ=255)

                                                                                                                         gTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGTAAAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAgggg                                                                                                                       >  1:733276/1‑251 (MQ=255)

                                                                                                                            ttattCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGcac                                                                                                                             >  1:371869/1‑241 (MQ=255)

                                                                                                                             tattCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTAt                                                                                                                  <  1:460841/251‑1 (MQ=255)

                                                                                                                              attCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGc                                                                                                                                                                                                                                                <  1:572459/125‑1 (MQ=255)

                                                                                                                              attCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTAtt                                                                                                                 <  1:898518/251‑1 (MQ=255)

                                                                                                                              attCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTAtt                                                                                                                 <  1:460794/251‑1 (MQ=255)

                                                                                                                              attCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTAtt                                                                                                                 >  1:267212/1‑251 (MQ=255)

                                                                                                                              attCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGAACCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTAtt                                                                                                                 <  1:305970/251‑1 (MQ=255)

                                                                                                                                 cGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAAt                                                                                                              <  1:642953/251‑1 (MQ=255)

                                                                                                                                   gACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATc                                                                                                             <  1:308703/250‑1 (MQ=255)

                                                                                                                                        ggCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAg                                                                                                       >  1:18746/1‑251 (MQ=255)

                                                                                                                                               cagcGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGaaaaa                                                                                                  <  1:499276/249‑1 (MQ=255)

                                                                                                                                                  cGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAGCATCAACTATGTGGTAGGCGCCAGCATCGCCGGTTTCGTGAAAGTCACCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAacac                                                                                              <  1:802966/250‑1 (MQ=255)

                                                                                                                                                  cGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAacac                                                                                              <  1:493659/250‑1 (MQ=255)

                                                                                                                                                      tGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAgg                                                                                                                         >  1:676603/1‑219 (MQ=255)

                                                                                                                                                      tGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATc                                                                                                             <  1:176429/231‑1 (MQ=255)

                                                                                                                                                        cAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTAtt                                                                                                                 <  1:640956/225‑1 (MQ=255)

                                                                                                                                                            tggtgTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATg                                                                                   <  1:324699/251‑1 (MQ=255)

                                                                                                                                                             ggtgTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATgtgg                                                                                                                                                                                <  1:116562/157‑1 (MQ=255)

                                                                                                                                                              gtgtGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCAc                                                                                                                                                                                                                                     <  1:177889/104‑1 (MQ=255)

                                                                                                                                                              gtgtGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGa                                                                                  >  1:303006/1‑250 (MQ=255)

                                                                                                                                                               tgtgGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGAc                                                                                 <  1:754245/250‑1 (MQ=255)

                                                                                                                                                                         tCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGAt                                                                      <  1:707635/251‑1 (MQ=255)

                                                                                                                                                                          ccGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATc                                                                     <  1:849782/251‑1 (MQ=255)

                                                                                                                                                                          ccGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGACCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATc                                                                     <  1:530917/251‑1 (MQ=255)

                                                                                                                                                                            ggCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATgtgg                                                                                                                                                                                <  1:579280/142‑1 (MQ=255)

                                                                                                                                                                             gCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTg                                                                   >  1:594619/1‑250 (MQ=255)

                                                                                                                                                                             gCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGCATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTg                                                                   >  1:474788/1‑250 (MQ=255)

                                                                                                                                                                               ttAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGccc                                                                <  1:64697/251‑1 (MQ=255)

                                                                                                                                                                                tAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATgtgg                                                                                                                                                                                <  1:170605/138‑1 (MQ=255)

                                                                                                                                                                                   aaaTGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTcc                                                             <  1:332245/250‑1 (MQ=255)

                                                                                                                                                                                     aTGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACCCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCaa                                                          <  1:251837/251‑1 (MQ=255)

                                                                                                                                                                                        tCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAg                                                        <  1:502522/250‑1 (MQ=255)

                                                                                                                                                                                         cGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAgag                                                      <  1:632641/251‑2 (MQ=255)

                                                                                                                                                                                                      aCGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAacac                                                                                              <  1:237540/198‑1 (MQ=255)

                                                                                                                                                                                                      aCGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCca                                         >  1:708453/1‑251 (MQ=255)

                                                                                                                                                                                                       cGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATgtgg                                                                                                                                                                                <  1:434020/115‑1 (MQ=255)

                                                                                                                                                                                                       cGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGa                                                                                                                                                     >  1:97802/1‑142 (MQ=255)

                                                                                                                                                                                                       cGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCcaa                                        >  1:755353/1‑251 (MQ=255)

                                                                                                                                                                                                       cGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCcaa                                        >  1:218091/1‑251 (MQ=255)

                                                                                                                                                                                                       cGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGAGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGAcgccgccgc                                           >  1:441059/1‑248 (MQ=255)

                                                                                                                                                                                                        gTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGAc                                                                                 >  1:660327/1‑209 (MQ=255)

                                                                                                                                                                                                         tttAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCcaatc                                      <  1:624935/251‑1 (MQ=255)

                                                                                                                                                                                                             aGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAAtt                                  >  1:618993/1‑251 (MQ=255)

                                                                                                                                                                                                                   aCCCGCGAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATg                            <  1:509788/251‑1 (MQ=255)

                                                                                                                                                                                                                       gcgAAGAAGTGGACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAAt                                                                                                              <  1:126160/165‑1 (MQ=255)

                                                                                                                                                                                                                                  gACACGAAATTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAgg              <  1:487736/250‑1 (MQ=255)

                                                                                                                                                                                                                                        aaaTTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAgagg                                                     <  1:127098/205‑3 (MQ=255)

                                                                                                                                                                                                                                        aaaTTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACat       <  1:582035/251‑1 (MQ=255)

                                                                                                                                                                                                                                        aaaTTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACat       >  1:476734/1‑251 (MQ=255)

                                                                                                                                                                                                                                        aaaTTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACat       >  1:260475/1‑251 (MQ=255)

                                                                                                                                                                                                                                        aaaTTACACAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACat       >  1:99146/1‑251 (MQ=255)

                                                                                                                                                                                                                                             acacAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGccc                                                                <  1:427755/189‑1 (MQ=255)

                                                                                                                                                                                                                                             acacAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATg                            <  1:200015/225‑1 (MQ=255)

                                                                                                                                                                                                                                             acacAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACATTAttt  <  1:733515/251‑1 (MQ=255)

                                                                                                                                                                                                                                             acacAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACATTAttt  >  1:620654/1‑251 (MQ=255)

                                                                                                                                                                                                                                             acacAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACATTAttt  <  1:766351/251‑1 (MQ=255)

                                                                                                                                                                                                                                             acacAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACATTAttt  <  1:315437/251‑1 (MQ=255)

                                                                                                                                                                                                                                             acacAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACATTAttt  <  1:20317/251‑1 (MQ=255)

                                                                                                                                                                                                                                             acacAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACATTAttt  >  1:898565/1‑251 (MQ=255)

                                                                                                                                                                                                                                             acacAGCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACATTAttt  >  1:131306/1‑251 (MQ=255)

                                                                                                                                                                                                                                                  gCATCATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGAGGACgccgccgcc                                          <  1:287977/206‑1 (MQ=255)

CCAAAACGAATTGGCATTGAGCGACGCGCAAACCCTTATCGCCAACGACGTACAAGTGGTGGCGGAAGGCGCCAATATGCCAACCACCATCGACGCCACCAATGCCTTCATTGACGCCGGCGTATTATTCGGACCGGGCAAAGCAGCGAATGCAGGTGGTGTGGCAACTTCCGGCTTAGAAATGTCGCAAAATGCACAACGTTTAAGCTGGACCCGCGAAGAAGTGGACACGAAATTACACAGCATAATGCTGGATATTCACGCTAACTGT‑AAAAAATACGGCTCCAACGCCCAAGGCAACATCAACTATGTGGTGGGCGCCAACATCGCCGGTTTCGTGAAAGTCGCCGATGCCATGTTGGCACAAGGGGTGTATTAATCGGTCAGAAAAAACACCGTTGAAAATGACCGCACTTTGATCTGCCCTCCCAAAGACGACGCCGCCGCCAATCAATTAAAATGCAGGTTTTTATAGGAAAACATTATTT  >  1/168496‑168983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`