Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	68,483	G→C	intergenic (+95/‑62)	unknown → / → unknown	Protein SprT/Serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	68,483	0	G	C	100.0%	166.5 / NA	58	intergenic (+95/‑62)	unknown/unknown	Protein SprT/Serine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (24/34);  total (24/34)

GATTTAACCTCTGTGCAAGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCAGTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTGTTTTTGCCGATTAATGCGGGTATGGGGGGATTTTGGCCGTTAATTGTGATGGCGATTTTAGTAGGACCAATGACTTATTTGGCACATCGCGGTTT  >  1/68239‑68729                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gATTTAACCTCTGTGCAAGGCAAAACCTTCACTTACCGATGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCtt                                                                                                                                                                                                                                                  >  1:672102/1‑251 (MQ=255)     tAACCTCTGTGCAAGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTa                                                                                                                                                                                                                                              <  1:114348/251‑1 (MQ=255)     tAACCTCTGTGCAAGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTa                                                                                                                                                                                                                                              <  1:571948/251‑1 (MQ=255)     tAACCTCTGTGCAAGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTa                                                                                                                                                                                                                                              <  1:278252/251‑1 (MQ=255)     tAACCTCTGTGCAAGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTa                                                                                                                                                                                                                                              <  1:242488/251‑1 (MQ=255)                cAAGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGtactac                                                                                                                                                                                                                                    <  1:415154/250‑1 (MQ=255)                 aaGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATGCTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTActacta                                                                                                                                                                                                                                  <  1:413824/251‑1 (MQ=255)                 aaGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTActacta                                                                                                                                                                                                                                  <  1:444775/251‑1 (MQ=255)                 aaGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTActacta                                                                                                                                                                                                                                  <  1:786858/251‑1 (MQ=255)                 aaGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTActacta                                                                                                                                                                                                                                  <  1:106746/251‑1 (MQ=255)                 aaGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTActacta                                                                                                                                                                                                                                  <  1:277083/251‑1 (MQ=255)                   ggCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTActacta                                                                                                                                                                                                                                  <  1:568184/249‑1 (MQ=255)                   ggCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTAt                                                                                                                                                                                                                                 >  1:503066/1‑250 (MQ=255)                    gCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATc                                                                                                                                                                                                                                <  1:56013/250‑1 (MQ=255)                    gCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATc                                                                                                                                                                                                                                <  1:372383/250‑1 (MQ=255)                          ccTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGtactac                                                                                                                                                                                                                                    >  1:299157/1‑240 (MQ=255)                                cTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTActacta                                                                                                                                                                                                                                  >  1:43975/1‑236 (MQ=255)                                    ccGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAatat                                                                                                                                                                                                               >  1:645093/1‑251 (MQ=255)                                                          cAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTActacta                                                                                                                                                                                                                                  >  1:755460/1‑210 (MQ=255)                                                          cAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATc                                                                                                                                                                                                                                <  1:564785/212‑1 (MQ=255)                                                           aaTTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATg                                                                                                                                                                                        >  1:488852/1‑251 (MQ=255)                                                           aaTTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGCGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGCTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTActacta                                                                                                                                                                                                                                  >  1:392930/1‑209 (MQ=255)                                                            aTTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAgg                                                                                                                                                                                                  <  1:234799/240‑1 (MQ=255)                                                                aGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAAc                                                                                                                                                                                                                                            <  1:689145/194‑1 (MQ=255)                                                                           tcATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAAc                                                                                                                                                                                                                                            >  1:277257/1‑183 (MQ=255)                                                                           tcATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATc                                                                                                                                                                                                                                <  1:538416/195‑1 (MQ=255)                                                                              taataaAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGaaaaaa                                                                                                                                                                     >  1:61950/1‑251 (MQ=255)                                                                                             tGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAg                                                                                                                                                                                                   >  1:65366/1‑206 (MQ=255)                                                                                                  gTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGtc                                                                                                                                                                                      <  1:700866/214‑1 (MQ=255)                                                                                                         atatTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAAc                                                                                                                                                                                                                                            <  1:322998/153‑1 (MQ=255)                                                                                                                        aTGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGtc                                                                                                                                                                                      >  1:862207/1‑192 (MQ=255)                                                                                                                                    gTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAAACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGatat                                                                                                                                                  >  1:451015/1‑216 (MQ=255)                                                                                                                                           tGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAgg                                                                                                        <  1:188334/251‑1 (MQ=255)                                                                                                                                            gggTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGtc                                                                                                                                                                                      <  1:71816/172‑1 (MQ=255)                                                                                                                                            gggTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAgg                                                                                                              <  1:632826/244‑1 (MQ=255)                                                                                                                                                 gttTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATc                                                                                                                                                                                                                                <  1:136162/125‑1 (MQ=255)                                                                                                                                                 gttTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAatat                                                                                                                                                                                                               >  1:141871/1‑142 (MQ=255)                                                                                                                                                 gttTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATaaa                                                                                                                                                                                                     >  1:644689/1‑152 (MQ=255)                                                                                                                                                 gttTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTa                                                                                                    >  1:178671/1‑249 (MQ=255)                                                                                                                                                 gttTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTa                                                                                                    >  1:578217/1‑249 (MQ=255)                                                                                                                                                 gttTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTa                                                                                                    >  1:18447/1‑249 (MQ=255)                                                                                                                                                  tttAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTg                                                                                                 <  1:220521/251‑1 (MQ=255)                                                                                                                                                      aaTATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAgg                                                                                                                                                                                                  <  1:231092/150‑1 (MQ=255)                                                                                                                                                         atTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGa                                                                                                             <  1:227697/232‑1 (MQ=255)                                                                                                                                                                      attaaaACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAAc                                                                                                                                                                                                                                            <  1:424296/92‑1 (MQ=255)                                                                                                                                                                        taaaACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTGTTTTTGCCGATTAATGCGGGTa                                                                           <  1:654844/251‑1 (MQ=255)                                                                                                                                                                                gTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAAc                                                                                                                                                                                                            >  1:542959/1‑114 (MQ=255)                                                                                                                                                                                 ttACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGgtg                                                                                                      <  1:857193/215‑1 (MQ=255)                                                                                                                                                                                                aaTTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTGTTTTTGCCGATTAATGCGGGTATGGGGGGATTTTGGCCGTTAATtg                                                   <  1:197037/251‑1 (MQ=255)                                                                                                                                                                                                      ctctAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTGAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTGTTTTTGCCGATTAATGCGGGTATGGGGGGATTTTGGCCGTTAATTGTGATgg                                             >  1:116/1‑251 (MQ=255)                                                                                                                                                                                                            aaGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAgg                                                                                                                                                                                                  >  1:21815/1‑96 (MQ=255)                                                                                                                                                                                                            aaGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTGTTTTTGCCGATTAATGCGGGTATGGGGGGATTTTGGCCGTTAATTGTGATGGCGAttt                                       >  1:122147/1‑251 (MQ=255)                                                                                                                                                                                                              gTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATc                                                                                                                                                                                                                                <  1:464028/64‑1 (MQ=255)                                                                                                                                                                                                                     ttCGATCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTGTTTTTGCCGATTAATGCGGGTATGGGGGGATTTTGGCCGTTAATTGTGATGGCGATTTTAGTAGGAc                              <  1:71978/251‑1 (MQ=255)                                                                                                                                                                                                                         aTCACAAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTGTTTTTGCCGATTAATGCGGGTATGGGGGGATTTTGGCCGTTAATTGTGATGGCGATTTTAGTAGGACCAAt                          >  1:128064/1‑251 (MQ=255)                                                                                                                                                                                                                            acaAATCTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTGTTTTTGCCGATTAATGCGGGTATGGGGGGATTTTGGCCGTTAATTGTGATGGCGATTTTAGTAGGACCAATGAc                       <  1:162019/251‑1 (MQ=255)                                                                                                                                                                                                                                  cTTTTAAATTCCTTTTGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGgtg                                                                                                      <  1:747762/166‑1 (MQ=255)                                                                                                                                                                                                                                                 tGCACTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTATGAGCAGGGGTACTGTTTTTGCCGATTAATGCGGGGATGGGGGGATTTTGGCCGTTAATTGTGATGGCGATTTTAGTAGGATCAATGACTTATTTTGCACATCGCGGttt  >  1:720661/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GATTTAACCTCTGTGCAAGGCAAAACCTTCACTTACCGTTGCGCCTGTCAAACCCATCAATTAAGTATTCGTCGTCATAATAAAATTCAACGTGATGGTGCAGTATATTTTTGCCGTAAATGCAAAACCCGGTTAAATTGGGTTGTTTAAATATTAACAGGTTAAATTAAAACTTGTTACAAAACTATCAAAATTATCTCTAAAAGTTATACTTCGATCACAAATCTTTTAAATTCCTTTTGCAGTCCCTTCGTAACAGTACTACTATCTGCGCAAAAATAAATATAACTTATAAAAGGAGACAATATGTCAAATCAAAATGAAAAAAAATGGCGTAAATTCGATATTATTTGGATGCTTAATCTGTTTGGCACGGCGGTAGGAGCAGGTGTACTGTTTTTGCCGATTAATGCGGGTATGGGGGGATTTTGGCCGTTAATTGTGATGGCGATTTTAGTAGGACCAATGACTTATTTGGCACATCGCGGTTT  >  1/68239‑68729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑