Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	129,898	C→T	R9W (CGG→TGG)	unknown →	Type IV fimbrial assembly protein PilC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	129,898	0	C	T	100.0%	199.6 / NA	68	R9W (CGG→TGG)	unknown	Type IV fimbrial assembly protein PilC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/29);  total (39/29)

TGCCAAAAGTGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAACGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGTGCCGCTGAAAAACAGCCTGCAAATTTTATTGCAAAATTGTACCAATA  >  1/129663‑130144                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       tGCCAAAAGTGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATc                                                                                                                                                                                                                                         <  1:85295/251‑1 (MQ=255) tGCCAAAAGTGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATc                                                                                                                                                                                                                                         <  1:650092/251‑1 (MQ=255)  accAAAAGTGCGGTTGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCGCAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCg                                                                                                                                                                                                                                        <  1:323618/250‑1 (MQ=255)   ccAAAAGTGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCg                                                                                                                                                                                                                                        <  1:301421/250‑1 (MQ=255)                ggAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGcaaaaa                                                                                                                                                                                                                           >  1:804021/1‑250 (MQ=255)                    aTGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGcaaaaacaaaa                                                                                                                                                                                                                      >  1:60804/1‑251 (MQ=255)                                       ttGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGaa                                                                                                                                                                                                   >  1:355516/1‑251 (MQ=255)                                                aGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACt                                                                                                                                                                                                                                     >  1:25569/1‑208 (MQ=255)                                                aGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCg                                                                                                                                                                                          >  1:325239/1‑251 (MQ=255)                                                aGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCg                                                                                                                                                                                          >  1:348675/1‑251 (MQ=255)                                                     aCAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATgg                                                                                                                                                                                      >  1:886662/1‑250 (MQ=255)                                                     aCAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATgg                                                                                                                                                                                      >  1:587857/1‑250 (MQ=255)                                                               tgtCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAg                                                                                                                                                                           >  1:376905/1‑251 (MQ=255)                                                                 tCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAgc                                                                                                                                                                          <  1:14923/250‑1 (MQ=255)                                                                   ggggTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAgctg                                                                                                                                                                        >  1:868665/1‑250 (MQ=255)                                                                    gggTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCATGGCGGAAACGGAGGCGATGGCACAACAACAgctg                                                                                                                                                                        >  1:857750/1‑249 (MQ=255)                                                                         tATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCAt                                                                                                                                                                 >  1:50397/1‑251 (MQ=255)                                                                          aTCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATg                                                                                                                                                                >  1:513157/1‑251 (MQ=255)                                                                          aTCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATg                                                                                                                                                                <  1:271793/251‑1 (MQ=255)                                                                          aTCAATTCCTGCAACCGAATTTACAACACCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGcaaaaacaaaaa                                                                                                                                                                                                                     <  1:440159/198‑1 (MQ=255)                                                                                       aCCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAAc                                                                                                                                                   <  1:723575/251‑1 (MQ=255)                                                                                        ccGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACa                                                                                                                                                  >  1:454763/1‑251 (MQ=255)                                                                                           aaTTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCa                                                                                                                                               >  1:11947/1‑251 (MQ=255)                                                                                                    caTCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTacaac                                                                                                                                      <  1:305412/251‑1 (MQ=255)                                                                                                    caTCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTacaac                                                                                                                                      <  1:716625/251‑1 (MQ=255)                                                                                                    caTCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTacaac                                                                                                                                      <  1:365576/251‑1 (MQ=255)                                                                                                     aTCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTacaaca                                                                                                                                     >  1:659128/1‑251 (MQ=255)                                                                                                      tCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATg                                                                                                                                                                <  1:149729/223‑1 (MQ=255)                                                                                                          cAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAAc                                                                                                                                 <  1:180735/250‑1 (MQ=255)                                                                                                            gggCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTgg                                                                                                                              >  1:272430/1‑251 (MQ=255)                                                                                                              gCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGGACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCa                                                                                                                            >  1:488398/1‑251 (MQ=255)                                                                                                              gCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCa                                                                                                                            >  1:318615/1‑251 (MQ=255)                                                                                                               cTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCacaac                                                                                                                                                                                <  1:355722/198‑1 (MQ=255)                                                                                                               cTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCaa                                                                                                                           <  1:491924/251‑1 (MQ=255)                                                                                                                                  gATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGaaaa                                                                                                        >  1:192692/1‑251 (MQ=255)                                                                                                                                   aTTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGaaaaa                                                                                                       >  1:261092/1‑251 (MQ=255)                                                                                                                                   aTTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGaaaaa                                                                                                       >  1:390965/1‑251 (MQ=255)                                                                                                                                           cAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACa                                                                                               <  1:118387/251‑1 (MQ=255)                                                                                                                                                  cTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCa                                                                                         >  1:469585/1‑250 (MQ=255)                                                                                                                                                  cTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCAc                                                                                        <  1:207269/251‑1 (MQ=255)                                                                                                                                                             cTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGc                                                                                                                                                               >  1:302070/1‑169 (MQ=255)                                                                                                                                                                     cGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTcc                                                                              >  1:709481/1‑242 (MQ=255)                                                                                                                                                                     cGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTACCAACTGGCg                                                                     >  1:834099/1‑251 (MQ=255)                                                                                                                                                                       gCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCCTTCATGCGGGTTGCAAATCAt                                                                                                                                                 <  1:853336/173‑1 (MQ=255)                                                                                                                                                                          caacaGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACg                                                                  >  1:465503/1‑249 (MQ=255)                                                                                                                                                                          caacaGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACg                                                                  >  1:633274/1‑249 (MQ=255)                                                                                                                                                                               gATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATc                                                                                             <  1:870034/217‑1 (MQ=255)                                                                                                                                                                                aTTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTAc                                                           <  1:483446/250‑1 (MQ=255)                                                                                                                                                                                  ttAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAg                                                        <  1:298256/251‑1 (MQ=255)                                                                                                                                                                                   tAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAgc                                                       <  1:729854/251‑1 (MQ=255)                                                                                                                                                                                    aaCCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAgcc                                                      <  1:163393/251‑1 (MQ=255)                                                                                                                                                                                         aaaTCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAACCTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGt                                                 >  1:442745/1‑251 (MQ=255)                                                                                                                                                                                            tCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTAGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGTGc                                               >  1:425152/1‑250 (MQ=255)                                                                                                                                                                                              cAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTAc                                                           <  1:743366/236‑1 (MQ=255)                                                                                                                                                                                                   ggTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAAc                                                                                                                                                                                                 <  1:177348/97‑1 (MQ=255)                                                                                                                                                                                                   ggTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAgc                                                       >  1:372100/1‑235 (MQ=255)                                                                                                                                                                                                    gtgtTAGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCAc                                                                                        >  1:567926/1‑201 (MQ=255)                                                                                                                                                                                                         aGGACAAACTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAg                                                                                                                      <  1:8826/166‑1 (MQ=255)                                                                                                                                                                                                              aaaCTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGc                                                                                                                                                               <  1:755682/120‑1 (MQ=255)                                                                                                                                                                                                              aaaCTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGTGCCGCTGAAAAAc                                    >  1:685384/1‑243 (MQ=255)                                                                                                                                                                                                                 cTCATGATTAAATTGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCAc                                                                                        >  1:817622/1‑188 (MQ=255)                                                                                                                                                                                                                             ttGAAACTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGTGCCGCTGAAAAACAGCCTGCAAATTTTATTGCaaaa             >  1:20288/1‑251 (MQ=255)                                                                                                                                                                                                                                   cTGTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGTGCCGCTGAAAAACAGCCTGCAAATTTTATTGCAAAATTGTa        >  1:3133/1‑250 (MQ=255)                                                                                                                                                                                                                                     gTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCAc                                                                                        >  1:354316/1‑168 (MQ=255)                                                                                                                                                                                                                                     gTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGc                                                                 <  1:153746/191‑1 (MQ=255)                                                                                                                                                                                                                                     gTTTCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGTGCCGCTGAAAAACAGCCTGCAAATTTTATTGCAAAATTGTACCa     >  1:175339/1‑251 (MQ=255)                                                                                                                                                                                                                                      tttCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGTGCCGCTGAAAAACAGCCTGCa                            >  1:17751/1‑227 (MQ=255)                                                                                                                                                                                                                                        tCAATGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGTGCCGCTGAAAAACAGCCTGCAAATTTTATTGCAAAATTGTACCAata  <  1:190063/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       TGCCAAAAGTGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACCGAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCACAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAAATTGAAACTGTTTCAACGGCGCGCCGTTAATCGACTACAGCAAAAACAAAAAGGCTTAATCGTGGCGGAAACGGAGGCGATGGCACAACAACAGCTGCTTTCATGCGGGTTGCAAAACATCAAATTACAACAAAACTGGCAATTTAGCCACAAACCGAAAAATGCCGACATCTGCACGCTGCTTTCCCAACTGGCGACGCTGTTACAAGCCGCCGTGCCGCTGAAAAACAGCCTGCAAATTTTATTGCAAAATTGTACCAATA  >  1/129663‑130144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑