Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	103,611	C→A	T212N (ACC→AAC)	unknown →	Transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	103,611	0	C	A	100.0%	76.9 / NA	30	T212N (ACC→AAC)	unknown	Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/12);  total (18/12)

GCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAAGTGGTTAAATTGCACCTTTTTAAATTTAGCCACTTTTTTTTCTAATTTTGATTTTTCCTTTTATCCTCTAAATAAAAAAGGGCTGAAATTTCTTTCAACCCTTTATTTCATTGATTCTTTCCCGCTTAATTC  >  1/103372‑103851                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 gcgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatca                                                                                                                                                                                                                                       >  1:517750/1‑251 (MQ=21)  cgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatcat                                                                                                                                                                                                                                      <  1:66536/251‑1 (MQ=21)    cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg                                                                                                                                                                                                                                    >  1:408613/1‑251 (MQ=255)    cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg                                                                                                                                                                                                                                    >  1:606212/1‑251 (MQ=255)                  tttGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                                                                                                                                                      <  1:651874/249‑1 (MQ=21)                           aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa                                                                                                                                                                                                              <  1:855946/250‑1 (MQ=21)                           aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa                                                                                                                                                                                                              <  1:252470/250‑1 (MQ=21)                              gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAGGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                                                                                                                                                           >  1:450671/1‑250 (MQ=21)                              gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                                                                                                                                                           >  1:106359/1‑250 (MQ=21)                              gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                                                                                                                                                           >  1:745705/1‑250 (MQ=21)                              gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                                                                                                                                                           >  1:637457/1‑250 (MQ=21)                                gTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGca                                                                                                                                                                                                                       >  1:13074/1‑236 (MQ=21)                                     aaTGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAttt                                                                                                                                                                                                   <  1:822715/251‑1 (MQ=18)                                        gcgcCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTaaa                                                                                                                                                                                                >  1:540019/1‑251 (MQ=18)                                                caATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggcttt                                                                                                                                                                                        >  1:881059‑M1/1‑244 (MQ=255)                                                  aTCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaa                                                                                                                                                                                      >  1:684641‑M1/1‑242 (MQ=255)                                                           ggCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgtttttt                                                                                                                                                                             >  1:410584‑M1/1‑233 (MQ=255)                                                              gATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                                                                                                                                                      >  1:729910/1‑207 (MQ=18)                                                                tCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                                                                                                                                                      <  1:597917/205‑1 (MQ=18)                                                                                        gaaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag                                                                                                                                                >  1:346617‑M1/1‑204 (MQ=255)                                                                                         aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagt                                                                                                                                               <  1:645803‑M1/251‑49 (MQ=255)                                                                                         aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag                                                                                                                                                <  1:697688‑M1/250‑48 (MQ=255)                                                                                              gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAtt                                                                                                                                                                                                    >  1:755770/1‑193 (MQ=17)                                                                                              gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta                                                                                                                                          >  1:262715‑M1/1‑198 (MQ=255)                                                                                              gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta                                                                                                                                          >  1:285583‑M1/1‑198 (MQ=255)                                                                                              gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCACCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta                                                                                                                                          >  1:663178‑M1/1‑198 (MQ=255)                                                                                                  aaaGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaac                                                                                                                                      <  1:489533‑M1/251‑58 (MQ=255)                                                                                                                                                                                                                        caactaacccaagctgaagccgaaaacctgcAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaaccgccctttttagattttagccactttttttc                                                                                                       <  1:160772‑M1/150‑89 (MQ=255)                                                                                                                                                                                                                             aacccaagctgaagccgaaaaCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaaccgccctttttagattttagccactttttttctaaaactaactttaacgacaaaaccacccaataaaaaagggccaaaacttctttcagccctttatttcattgattctgccccgcttaattc            <  1:570623‑M1/241‑180 (MQ=255)                                                                                                                                                                                                                             aacccaagctgaagccgaaaaCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaaccgccctttttagattttagccactttttttctaaaactaactttaacgacaaaaccacccaataaaaaagggccaaaacttctttcagccctttatttcattgattctgccccgcttaatt             >  1:33905‑M1/11‑72 (MQ=255)                                                                                                                                                                                                                             aacccaagctgaagccgaaaaCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaaccgccctttttagattttagccactttttttctaaaactaactttaacgacaaaaccacccaataaaaaagggccaaaacttctttcagccctttatttcattgattctgccccgcttaatt             >  1:58974‑M1/11‑72 (MQ=255)                                                                                                                                                                                                                                        aaGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaaccgccctttttagattttagccactttttttctaaaactaactttaacgacaaaaccacccaataaaaaagggccaaaacttctttcagccctttatttcattgattctgccccgcttaattccgtttaattc  <  1:191275‑M1/250‑190 (MQ=255)                                                                                                                                                                                                                                        aaGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaaccgccctttttagattttagccactttttttctaaaactaactttaacgacaaaaccacccaataaaaaagggccaaaacttctttcagccctttatttcattgattctgccccgcttaattccgttcaattc  <  1:861979‑M1/250‑190 (MQ=255)                                                                                                                                                                                                                                           ccGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaaccgccctttttagattttagccactttttttctaaaactaactttaacgacaaaaccacccaataaaaaagggccaaaacttctttcagcc                                            <  1:588687‑M1/205‑148 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 GCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAAGTGGTTAAATTGCACCTTTTTAAATTTAGCCACTTTTTTTTCTAATTTTGATTTTTCCTTTTATCCTCTAAATAAAAAAGGGCTGAAATTTCTTTCAACCCTTTATTTCATTGATTCTTTCCCGCTTAATTC  >  1/103372‑103851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑