Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	103,576	C→T	Y200Y (TAC→TAT)	unknown →	Transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	103,576	0	C	T	100.0%	71.2 / NA	29	Y200Y (TAC→TAT)	unknown	Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/10);  total (19/10)

ATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAA  >  1/103333‑103719                                                                                                                                                                                                                                                    |                                                                                                                                                 aTACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaa                                                                                                                                           >  1:29284/1‑249 (MQ=37)         aaaCGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaatccaact                                                                                                                                    <  1:179098/250‑10 (MQ=25)             gTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaatccaactaacc                                                                                                                                >  1:566648/1‑237 (MQ=25)                                        gcgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatca                                                                                                   >  1:517750/1‑251 (MQ=21)                                         cgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatcat                                                                                                  <  1:66536/251‑1 (MQ=21)                                           cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAACCCaagc                                                                                                                           <  1:557006/225‑3 (MQ=21)                                           cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg                                                                                                >  1:408613/1‑251 (MQ=255)                                           cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg                                                                                                >  1:606212/1‑251 (MQ=255)                                                         tttGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                  <  1:651874/249‑1 (MQ=21)                                                                  aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa                                                                          <  1:855946/250‑1 (MQ=21)                                                                  aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa                                                                          <  1:252470/250‑1 (MQ=21)                                                                     gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAGGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:450671/1‑250 (MQ=21)                                                                     gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:106359/1‑250 (MQ=21)                                                                     gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:745705/1‑250 (MQ=21)                                                                     gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:637457/1‑250 (MQ=21)                                                                       gTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGca                                                                                   >  1:13074/1‑236 (MQ=21)                                                                            aaTGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAttt                                                               <  1:822715/251‑1 (MQ=18)                                                                               gcgcCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTaaa                                                            >  1:540019/1‑251 (MQ=18)                                                                                  cccaacaATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAACCCaagct                                                                                                                          >  1:207977/5‑184 (MQ=18)                                                                                       caATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggcttt                                                    >  1:881059‑M1/1‑244 (MQ=255)                                                                                         aTCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaa                                                  >  1:684641‑M1/1‑242 (MQ=255)                                                                                                  ggCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgtttttt                                         >  1:410584‑M1/1‑233 (MQ=255)                                                                                                     gATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                  >  1:729910/1‑207 (MQ=18)                                                                                                       tCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                  <  1:597917/205‑1 (MQ=18)                                                                                                                               gaaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag            >  1:346617‑M1/1‑204 (MQ=255)                                                                                                                                aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagt           <  1:645803‑M1/251‑49 (MQ=255)                                                                                                                                aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag            <  1:697688‑M1/250‑48 (MQ=255)                                                                                                                                     gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccacc                                                                                                                                             >  1:305909/1‑117 (MQ=17)                                                                                                                                     gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAtt                                                                >  1:755770/1‑193 (MQ=17)                                                                                                                                     gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta      >  1:285583‑M1/1‑198 (MQ=255)                                                                                                                                     gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta      >  1:262715‑M1/1‑198 (MQ=255)                                                                                                                                     gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCACCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta      >  1:663178‑M1/1‑198 (MQ=255)                                                                                                                                         aaaGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaac  <  1:489533‑M1/251‑58 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                 ATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAA  >  1/103333‑103719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑