Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	103,566	G→A	R197K (AGA→AAA)	unknown →	Transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	103,566	0	G	A	100.0%	92.3 / NA	37	R197K (AGA→AAA)	unknown	Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/12);  total (25/12)

ACGGGCAAGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAA  >  1/103320‑103719                                                                                                                                                                                                                                                       |                                                                                                                                                           aCGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaaga                                                                                                                                                         <  1:469540/250‑1 (MQ=37)  cGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaagaag                                                                                                                                                       <  1:653321/251‑3 (MQ=37)        ggcgcCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATat                                                                                                                                                  >  1:69533/2‑249 (MQ=255)         gcgcCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatc                                                                                                                                                 >  1:71945/1‑248 (MQ=37)              aTACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaa                                                                                                                                           >  1:29284/1‑249 (MQ=37)                      aaaCGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaatccaact                                                                                                                                    <  1:179098/250‑10 (MQ=25)                          gTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaatccaactaacc                                                                                                                                >  1:566648/1‑237 (MQ=25)                                gCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAgaagaa                                                                                                                                                     <  1:111799/223‑1 (MQ=25)                                                     gcgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatca                                                                                                   >  1:517750/1‑251 (MQ=21)                                                      cgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatcat                                                                                                  <  1:66536/251‑1 (MQ=21)                                                        cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAACCCaagc                                                                                                                           <  1:557006/225‑3 (MQ=21)                                                        cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg                                                                                                >  1:408613/1‑251 (MQ=255)                                                        cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg                                                                                                >  1:606212/1‑251 (MQ=255)                                                          aTTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaagaag                                                                                                                                                       >  1:838869/1‑193 (MQ=21)                                                                  gTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaaga                                                                                                                                                         <  1:313488/185‑1 (MQ=25)                                                                    tttttGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATat                                                                                                                                                  >  1:117642/5‑189 (MQ=255)                                                                      tttGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                  <  1:651874/249‑1 (MQ=21)                                                                               aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa                                                                          <  1:252470/250‑1 (MQ=21)                                                                               aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa                                                                          <  1:855946/250‑1 (MQ=21)                                                                                  gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAGGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:450671/1‑250 (MQ=21)                                                                                  gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:745705/1‑250 (MQ=21)                                                                                  gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:106359/1‑250 (MQ=21)                                                                                  gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:637457/1‑250 (MQ=21)                                                                                    gTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGca                                                                                   >  1:13074/1‑236 (MQ=21)                                                                                         aaTGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAttt                                                               <  1:822715/251‑1 (MQ=18)                                                                                            gcgcCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTaaa                                                            >  1:540019/1‑251 (MQ=18)                                                                                               cccaacaATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAACCCaagct                                                                                                                          >  1:207977/5‑184 (MQ=18)                                                                                                    caATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggcttt                                                    >  1:881059‑M1/1‑244 (MQ=255)                                                                                                      aTCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaa                                                  >  1:684641‑M1/1‑242 (MQ=255)                                                                                                               ggCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgtttttt                                         >  1:410584‑M1/1‑233 (MQ=255)                                                                                                                  gATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                  >  1:729910/1‑207 (MQ=18)                                                                                                                    tCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                  <  1:597917/205‑1 (MQ=18)                                                                                                                                            gaaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag            >  1:346617‑M1/1‑204 (MQ=255)                                                                                                                                             aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagt           <  1:645803‑M1/251‑49 (MQ=255)                                                                                                                                             aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag            <  1:697688‑M1/250‑48 (MQ=255)                                                                                                                                                  gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccacc                                                                                                                                             >  1:305909/1‑117 (MQ=17)                                                                                                                                                  gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAtt                                                                >  1:755770/1‑193 (MQ=17)                                                                                                                                                  gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta      >  1:262715‑M1/1‑198 (MQ=255)                                                                                                                                                  gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta      >  1:285583‑M1/1‑198 (MQ=255)                                                                                                                                                  gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCACCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta      >  1:663178‑M1/1‑198 (MQ=255)                                                                                                                                                      aaaGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaac  <  1:489533‑M1/251‑58 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                           ACGGGCAAGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAA  >  1/103320‑103719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑