Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	103,534	C→T	N186N (AAC→AAT)	unknown →	Transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	103,534	0	C	T	100.0%	137.2 / NA	52	N186N (AAC→AAT)	unknown	Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/21);  total (31/21)

GTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAA  >  1/103293‑103719                                                                                                                                                                                                                                                  |                                                                                                                                                                                           gTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACggg                                                                                                                                                                                   >  1:881176/1‑251 (MQ=255)        tGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGc                                                                                                                                                                            <  1:189867/251‑1 (MQ=255)            ggCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCAt                                                                                                                                                                        >  1:791780/1‑251 (MQ=255)             gCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGc                                                                                                                                                                            <  1:180760/246‑1 (MQ=255)              cGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCAtt                                                                                                                                                                       <  1:309408/250‑1 (MQ=255)                            aCGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaaga                                                                                                                                                         <  1:469540/250‑1 (MQ=37)                             cGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaagaag                                                                                                                                                       <  1:653321/251‑3 (MQ=37)                                   ggcgcCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATat                                                                                                                                                  >  1:69533/2‑249 (MQ=255)                                    gcgcCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatc                                                                                                                                                 >  1:71945/1‑248 (MQ=37)                                      gcCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGAc                                                                                                                                                                                      <  1:456925/211‑1 (MQ=255)                                         aTACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaa                                                                                                                                           >  1:29284/1‑249 (MQ=37)                                           aCTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGc                                                                                                                                                                            <  1:385366/216‑1 (MQ=255)                                                 aaaCGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaatccaact                                                                                                                                    <  1:179098/250‑10 (MQ=25)                                                   aCGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGAc                                                                                                                                                                 >  1:647513/1‑219 (MQ=255)                                                     gTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaatccaactaacc                                                                                                                                >  1:566648/1‑237 (MQ=25)                                                           gCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGCTATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGAc                                                                                                                                                                 >  1:738643/1‑211 (MQ=255)                                                           gCATAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAgaagaa                                                                                                                                                     <  1:111799/223‑1 (MQ=25)                                                                                gcgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatca                                                                                                   >  1:517750/1‑251 (MQ=21)                                                                                 cgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatcat                                                                                                  <  1:66536/251‑1 (MQ=21)                                                                                   cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAACCCaagc                                                                                                                           <  1:557006/225‑3 (MQ=21)                                                                                   cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg                                                                                                >  1:408613/1‑251 (MQ=255)                                                                                   cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg                                                                                                >  1:606212/1‑251 (MQ=255)                                                                                     aTTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTGGAGc                                                                                                                                                                            >  1:430624/1‑174 (MQ=255)                                                                                     aTTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaagaag                                                                                                                                                       >  1:838869/1‑193 (MQ=21)                                                                                             gTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaaga                                                                                                                                                         <  1:313488/185‑1 (MQ=25)                                                                                               tttttGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATat                                                                                                                                                  >  1:117642/5‑189 (MQ=255)                                                                                                 tttGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                  <  1:651874/249‑1 (MQ=21)                                                                                                          aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa                                                                          <  1:855946/250‑1 (MQ=21)                                                                                                          aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa                                                                          <  1:252470/250‑1 (MQ=21)                                                                                                             gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAGGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:450671/1‑250 (MQ=21)                                                                                                             gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:106359/1‑250 (MQ=21)                                                                                                             gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:745705/1‑250 (MQ=21)                                                                                                             gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa                                                                       >  1:637457/1‑250 (MQ=21)                                                                                                               gTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGca                                                                                   >  1:13074/1‑236 (MQ=21)                                                                                                                    aaTGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAttt                                                               <  1:822715/251‑1 (MQ=18)                                                                                                                       gcgcCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTaaa                                                            >  1:540019/1‑251 (MQ=18)                                                                                                                          cccaacaATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAACCCaagct                                                                                                                          >  1:207977/5‑184 (MQ=18)                                                                                                                               caATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggcttt                                                    >  1:881059‑M1/1‑244 (MQ=255)                                                                                                                                 aTCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaa                                                  >  1:684641‑M1/1‑242 (MQ=255)                                                                                                                                          ggCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgtttttt                                         >  1:410584‑M1/1‑233 (MQ=255)                                                                                                                                             gATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                  >  1:729910/1‑207 (MQ=18)                                                                                                                                               tCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGc                                                                                                                                                                            <  1:388856/116‑1 (MQ=255)                                                                                                                                               tCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac                                                                                  <  1:597917/205‑1 (MQ=18)                                                                                                                                                                       gaaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag            >  1:346617‑M1/1‑204 (MQ=255)                                                                                                                                                                        aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagt           <  1:645803‑M1/251‑49 (MQ=255)                                                                                                                                                                        aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag            <  1:697688‑M1/250‑48 (MQ=255)                                                                                                                                                                             gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccacc                                                                                                                                             >  1:305909/1‑117 (MQ=17)                                                                                                                                                                             gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAtt                                                                >  1:755770/1‑193 (MQ=17)                                                                                                                                                                             gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta      >  1:262715‑M1/1‑198 (MQ=255)                                                                                                                                                                             gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta      >  1:285583‑M1/1‑198 (MQ=255)                                                                                                                                                                             gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCACCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta      >  1:663178‑M1/1‑198 (MQ=255)                                                                                                                                                                                 aaaGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaac  <  1:489533‑M1/251‑58 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                           GTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCATAAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAA  >  1/103293‑103719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑