Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	103,384	G→C	A136A (GCG→GCC)	unknown →	Transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	103,384	0	G	C	100.0%	206.0 / NA	74	A136A (GCG→GCC)	unknown	Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (40/34);  total (40/34)

GCACAGCAAGATATTAATGGCATTTCAATGATTAACTGCTTTTGTTCTATTAATGTTTCAGCCGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCG  >  1/103220‑103625                                                                                                                                                                      |                                                                                                                                                                                                                                                   tttaaagataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTaa                                                                                                                                                              >  1:294302/25‑251 (MQ=255)      agataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCg                                                                                                                                                         >  1:472056/20‑251 (MQ=255)       gataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGa                                                                                                                                                        >  1:81005/19‑251 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATaaa                                                                                                                                                    >  1:258055/14‑251 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAgg                                                                                                                                                           >  1:205766/14‑243 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAgg                                                                                                                                                           >  1:230175/14‑243 (MQ=255)                gaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCaaagaa                                                                                                                                                                       <  1:149309/218‑1 (MQ=255)                gaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatcta                                                                                                                                               >  1:114100/10‑251 (MQ=255)                 aatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATc                                                                                                                                                                                                                             <  1:152083/164‑1 (MQ=255)                  atgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatt                                                                                                                                             >  1:354224/8‑250 (MQ=255)                  atgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatt                                                                                                                                             >  1:330223/8‑250 (MQ=255)                    gtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAagag                                                                                                                                                                   >  1:885458/6‑227 (MQ=255)                    gtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatttg                                                                                                                                           >  1:368046/6‑248 (MQ=255)                     tatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTgg                                                                                                                                          <  1:490273/247‑1 (MQ=255)                        atCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac                                                                                                                                       <  1:839838/250‑3 (MQ=255)                        atCAATGATTAACTGCTGTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac                                                                                                                                       <  1:455163/250‑3 (MQ=255)                            aTGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGaa                                                                                                                                   >  1:606283/1‑251 (MQ=255)                             tGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAAc                                                                                                                                  <  1:342471/251‑1 (MQ=255)                              gATTATCTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTgg                                                                                                                                          <  1:552499/242‑1 (MQ=255)                              gATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTggg                                                                                                                                                                                                                                          >  1:237619/1‑146 (MQ=255)                              gATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCaa                                                                                                                                                                                                                           <  1:674060/161‑1 (MQ=255)                                      gCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAg                                                                                                                          >  1:817999/1‑250 (MQ=255)                                      gCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGAGATCAGATATTAGTg                                                                                                                                                                                     >  1:339937/1‑191 (MQ=255)                                       cTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATc                                                                                                                                                                                                                             >  1:375408/1‑150 (MQ=255)                                             ttccaTTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATggg                                                                                                                  <  1:759208/247‑1 (MQ=255)                                               ccaTTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGtt                                                                                                                >  1:444471/3‑251 (MQ=255)                                                     aTGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGAt                                                                                                                                                                                  <  1:775482/179‑1 (MQ=255)                                                     aTGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCg                                                                                                           >  1:344243/1‑250 (MQ=255)                                                              cgggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAAt                                                                                                  <  1:155213/248‑1 (MQ=255)                                                              cgggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATc                                                                                                 <  1:379065/249‑1 (MQ=255)                                                                ggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGTAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCg                                                                                                           <  1:437377/239‑1 (MQ=255)                                                                ggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAg                                                                                                                                                                                              >  1:101264/1‑156 (MQ=255)                                                                ggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCaa                                                                                               >  1:382988/1‑251 (MQ=255)                                                                  cTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac                                                                                                                                       <  1:670789/209‑3 (MQ=255)                                                                  cTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGc                                                                                                            >  1:266119/1‑236 (MQ=255)                                                                          gTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGt                                                                                                                                                                                      <  1:349927/154‑1 (MQ=255)                                                                          gTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGAt                                                                                                                                                                                  <  1:679385/158‑1 (MQ=255)                                                                          gTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATc                                                                                                                                                                             <  1:732307/163‑1 (MQ=255)                                                                          gTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACggg                                                                                     >  1:881176/1‑251 (MQ=255)                                                                           tttCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATg                                                                                                                                                                                                                         <  1:128107/118‑1 (MQ=255)                                                                            ttCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGGGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATggg                                                                                                                  >  1:451203/1‑220 (MQ=255)                                                                            ttCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATggg                                                                                                                  >  1:506601/1‑220 (MQ=255)                                                                                 tGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGc                                                                              <  1:189867/251‑1 (MQ=255)                                                                                     ggCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCAt                                                                          >  1:791780/1‑251 (MQ=255)                                                                                      gCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctattt                                                                                                                                            <  1:206160/184‑3 (MQ=255)                                                                                      gCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAAc                                                                                                                                  >  1:766923/1‑194 (MQ=255)                                                                                      gCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGc                                                                              <  1:180760/246‑1 (MQ=255)                                                                                       cGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCAtt                                                                         <  1:309408/250‑1 (MQ=255)                                                                                         tAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGAt                                                                                                                                                                                  >  1:43628/1‑143 (MQ=255)                                                                                                     aCGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaaga                                                           <  1:469540/250‑1 (MQ=37)                                                                                                      cGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaagaag                                                         <  1:653321/251‑3 (MQ=37)                                                                                                        ggCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCg                                                                                                           >  1:417633/1‑199 (MQ=255)                                                                                                            ggcgcCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAAc                                                                                                                                  <  1:309708/171‑1 (MQ=255)                                                                                                            ggcgcCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATat                                                    >  1:69533/2‑249 (MQ=255)                                                                                                             gcgcCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatc                                                   >  1:71945/1‑248 (MQ=37)                                                                                                               gcCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGAc                                                                                        <  1:456925/211‑1 (MQ=255)                                                                                                                  aTACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaa                                             >  1:29284/1‑249 (MQ=37)                                                                                                                    aCTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGc                                                                              <  1:385366/216‑1 (MQ=255)                                                                                                                          aaaCGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaatccaact                                      <  1:179098/250‑10 (MQ=25)                                                                                                                            aCGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACggcgc                                                                                                                             <  1:865581/161‑4 (MQ=255)                                                                                                                            aCGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGtt                                                                                                                <  1:87556/174‑1 (MQ=255)                                                                                                                            aCGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGAc                                                                   >  1:647513/1‑219 (MQ=255)                                                                                                                              gTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATatccaccaatccaactaacc                                  >  1:566648/1‑237 (MQ=25)                                                                                                                               ttATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAg                                                                                                                                                                                       <  1:250348/100‑1 (MQ=255)                                                                                                                                    gCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGCTATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGAc                                                                   >  1:738643/1‑211 (MQ=255)                                                                                                                                    gCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAgaagaa                                                       <  1:111799/223‑1 (MQ=25)                                                                                                                                               ggCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAg                                                                                                             >  1:636223/1‑159 (MQ=255)                                                                                                                                                          gcgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatca     >  1:517750/1‑251 (MQ=21)                                                                                                                                                           cgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatcat    <  1:66536/251‑1 (MQ=21)                                                                                                                                                             cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAACCCaagc                             <  1:557006/225‑3 (MQ=21)                                                                                                                                                             cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg  >  1:408613/1‑251 (MQ=255)                                                                                                                                                             cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg  >  1:606212/1‑251 (MQ=255)                                                                                                                                                               aTTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTGGAGc                                                                              >  1:430624/1‑174 (MQ=255)                                                                                                                                                               aTTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaagaag                                                         >  1:838869/1‑193 (MQ=21)                                                                                                                                                                      |                                                                                                                                                                                                                                                   GCACAGCAAGATATTAATGGCATTTCAATGATTAACTGCTTTTGTTCTATTAATGTTTCAGCCGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCG  >  1/103220‑103625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑