Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,106,085	T→C	Y245H (TAT→CAT)	unknown →	23S rRNA (uracil(747)‑C(5))‑methyltransferase (EC 2.1.1.189)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,106,085	0	T	C	100.0%	230.2 / NA	77	Y245H (TAT→CAT)	unknown	23S rRNA (uracil(747)‑C(5))‑methyltransferase (EC 2.1.1.189)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/29);  total (48/29)

TCAAAAGCTGCCGCAACTTGCCGCTATTTTAGAAGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGCTGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTATATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAACCCGCCCCGCCGTGGCGTTGGCAAGACACT  >  1/2105847‑2106314                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                      tCAAAAGCTGCCGCAACTTGCCGCTATTTTAGAAGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAg                                                                                                                                                                                                                           <  1:823941/251‑1 (MQ=255)     aaGCTGCCGCAACTTGCCGCTATTTTAGAAGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCt                                                                                                                                                                                                                       <  1:35/251‑1 (MQ=255)           ccGCAACTTGCCGCTATTTTAGAAGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTacaac                                                                                                                                                                                                                  <  1:598057/250‑1 (MQ=255)           ccGCAACTTGCCGCTATTTTAGAAGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTacaac                                                                                                                                                                                                                  <  1:489469/250‑1 (MQ=255)           ccGCAACTTGCCGCTATTTTAGAAGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTacaac                                                                                                                                                                                                                  <  1:759581/250‑1 (MQ=255)            cGCAACTTGCCGCTATTTTAGAAGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAg                                                                                                                                                                                                                <  1:820929/251‑1 (MQ=255)                          aTTTTAGAAGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCGGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGaa                                                                                                                                                                                                  >  1:758557/1‑251 (MQ=255)                                 aaGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAAccc                                                                                                                                                                                             <  1:220599/249‑1 (MQ=255)                                   ggggAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCg                                                                                                                                                                                         >  1:227906/1‑251 (MQ=255)                                      gAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGtc                                                                                                                                                                                       <  1:251310/250‑1 (MQ=255)                                                    ccTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGaaa                                                                                                                                                                        <  1:612546/251‑1 (MQ=255)                                                    ccTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGaaa                                                                                                                                                                        >  1:623667/1‑251 (MQ=255)                                                    ccTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGaaa                                                                                                                                                                        <  1:39261/251‑1 (MQ=255)                                                      tCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAAc                                                                                                                                                                                               >  1:193603/1‑226 (MQ=255)                                                      tCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAAt                                                                                                                                                                       >  1:57459/1‑250 (MQ=255)                                                           gAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAAccc                                                                                                                                                                                             >  1:313405/1‑223 (MQ=255)                                                           gAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATctccg                                                                                                                                                                  >  1:471734/1‑250 (MQ=255)                                                            aacaacACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATctccgcc                                                                                                                                                                <  1:373495/251‑1 (MQ=255)                                                                     cTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCAtt                                                                                                                                                       >  1:750090/1‑251 (MQ=255)                                                                             gAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTgccgccg                                                                                                                                                >  1:82993/1‑250 (MQ=255)                                                                                  gTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACAt                                                                                                                                          >  1:3956/1‑251 (MQ=255)                                                                                  gTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACAt                                                                                                                                          >  1:150692/1‑251 (MQ=255)                                                                                          gCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGca                                                                                                                                  >  1:659709/1‑251 (MQ=255)                                                                                            aTTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGcaca                                                                                                                                >  1:772784/1‑251 (MQ=255)                                                                                              tCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCacaac                                                                                                                              <  1:614895/251‑1 (MQ=255)                                                                                               ccGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCacaaca                                                                                                                             >  1:186328/1‑251 (MQ=255)                                                                                                    gTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTg                                                                                                                        >  1:680570/1‑251 (MQ=255)                                                                                                       cATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGc                                                                                                                     <  1:815647/251‑1 (MQ=255)                                                                                                       cATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACAGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGc                                                                                                                     <  1:839292/251‑1 (MQ=255)                                                                                                                  aaGGTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAg                                                                                                          <  1:368166/251‑1 (MQ=255)                                                                                                                    ggTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGtt                                                                                                        >  1:234925/1‑251 (MQ=255)                                                                                                                    ggTTTCTTCCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGtt                                                                                                        >  1:534377/1‑251 (MQ=255)                                                                                                                         cttcCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAAcc                                                                                                                                                                                              <  1:330728/160‑1 (MQ=255)                                                                                                                         cttcCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCgg                                                                                                                                    >  1:831299/1‑218 (MQ=255)                                                                                                                         cttcCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCacaac                                                                                                                              >  1:485028/1‑224 (MQ=255)                                                                                                                         cttcCAAACCAATCCGATGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGtt                                                                                                   >  1:692880/1‑251 (MQ=255)                                                                                                                                      ccgatGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGa                                                                                      <  1:266625/247‑1 (MQ=255)                                                                                                                                        gatGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGttt                                                                                                  >  1:558960/3‑237 (MQ=255)                                                                                                                                         atGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATg                                                                                    <  1:203482/249‑1 (MQ=255)                                                                                                                                           ggTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCACCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCgg                                                                                 >  1:424244/1‑251 (MQ=255)                                                                                                                                                     ggATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAg                                                                                                                                                                                                                           <  1:806756/103‑1 (MQ=255)                                                                                                                                                       aTTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAATCCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCt                                                                     <  1:485228/251‑1 (MQ=255)                                                                                                                                                       aTTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGc                                                                       >  1:654429/1‑249 (MQ=255)                                                                                                                                                        ttGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAg                                                                                                                                                                                                                           >  1:889406/1‑100 (MQ=255)                                                                                                                                                          gTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTaa                                                                  >  1:111406/1‑251 (MQ=255)                                                                                                                                                          gTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTaa                                                                  >  1:537882/1‑251 (MQ=255)                                                                                                                                                          gTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTaa                                                                  >  1:127073/1‑251 (MQ=255)                                                                                                                                                            aTGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAAcc                                                                <  1:722652/251‑1 (MQ=255)                                                                                                                                                                    gcgcAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGa                                                        >  1:626367/1‑251 (MQ=255)                                                                                                                                                                    gcgcAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGa                                                        >  1:270361/1‑251 (MQ=255)                                                                                                                                                                               ggTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAg                                                                                                                                                                                                                           >  1:558629/1‑77 (MQ=255)                                                                                                                                                                                gTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTTCAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCtgattt                                            >  1:801553/1‑251 (MQ=255)                                                                                                                                                                                gTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATctcc                                                                                                                                                                   >  1:470393/1‑132 (MQ=255)                                                                                                                                                                                gTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAg                                                                                                                            >  1:595486/1‑171 (MQ=255)                                                                                                                                                                                gTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCg                                                                                                               <  1:103918/184‑1 (MQ=255)                                                                                                                                                                                gTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAAcc                                                                >  1:201444/1‑231 (MQ=255)                                                                                                                                                                                gTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAACCCtgattt                                            >  1:13159/1‑251 (MQ=255)                                                                                                                                                                                gTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCtgattt                                            >  1:485059/1‑251 (MQ=255)                                                                                                                                                                                gTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCtgatt                                             <  1:895905/250‑1 (MQ=255)                                                                                                                                                                                    aaGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCtgatttgatt                                        <  1:455784/251‑1 (MQ=255)                                                                                                                                                                                        tCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCg                                    <  1:374375/251‑1 (MQ=255)                                                                                                                                                                                             cGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAAc                               <  1:784615/251‑1 (MQ=255)                                                                                                                                                                                              gATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTc                                  <  1:412636/247‑1 (MQ=255)                                                                                                                                                                                              gATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAAc                               >  1:125709/1‑250 (MQ=255)                                                                                                                                                                                               aTTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGttt                                                                                                  <  1:82267/182‑1 (MQ=255)                                                                                                                                                                                               aTTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAAcc                              >  1:27741/1‑250 (MQ=255)                                                                                                                                                                                                ttAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAAcc                                                                >  1:230667/1‑215 (MQ=255)                                                                                                                                                                                                ttAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAAcccg                            >  1:883990/1‑251 (MQ=255)                                                                                                                                                                                                ttAACACCCTTTGGGATTTATTTTGCGGTGTGAGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAAcccg                            >  1:210750/1‑251 (MQ=255)                                                                                                                                                                                                      cccTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTc                                  <  1:849765/239‑1 (MQ=255)                                                                                                                                                                                                        cTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACAt                                                                                                                                          >  1:492494/1‑133 (MQ=255)                                                                                                                                                                                                        cTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAACCCGCCccgccg                    >  1:599458/1‑251 (MQ=255)                                                                                                                                                                                                              gATTTATTTTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCgg                                                                                                                                    >  1:321768/1‑133 (MQ=255)                                                                                                                                                                                                                   atttTGCGGTGTGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAACCCGCCCCGCCGTGGCGTTGGCa         >  1:351547/1‑251 (MQ=255)                                                                                                                                                                                                                           gtgtGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAACCCGCCCCGCCGTGGCGTTGGCAAGACACt  >  1:465691/1‑250 (MQ=255)                                                                                                                                                                                                                           gtgtGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAACCCGCCCCGCCGTGGCGTTGGCAAGACACt  >  1:687009/1‑250 (MQ=255)                                                                                                                                                                                                                           gtgtGGGCGGCTTCGGATTACATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAACCCGCCCCGCCGTGGCGTTGGCAAGACACt  >  1:72815/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                      TCAAAAGCTGCCGCAACTTGCCGCTATTTTAGAAGGGGAAAAAGAGATTTTCCTCACGGAACAACACACTTTACCGGAATCGTTTAACGGCATTCCGCTGTTCATCCGCCCGCAAGGTTTCTTCCAAACCAATCCGCTGGTTGCAGAAGGATTGTATGGCACGGCGCAACAATGGGTTCAAGATCTGCCGATTAACACCCTTTGGGATTTATTTTGCGGTGTGGGCGGCTTCGGATTATATTGTTTACAAGCGCTACAACAGCGACATACGCAGAAAACCCTTCGTCTCACCGGCATTGAAATCTCCGCCTCCGCCATTGCCGCCGCCACATTATCGGCACAACAGCTTGGGCTTGCCGAACAGTTGAGTTTTCAATCTCTGGATGCGGCAAATTTTGCCTTAACCCAGGCGGAAAAACCTGATTTGATTATCGTCAACCCGCCCCGCCGTGGCGTTGGCAAGACACT  >  1/2105847‑2106314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑