Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	103,327	A→G	Q117Q (CAA→CAG)	unknown →	Transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	103,327	0	A	G	100.0%	155.5 / NA	56	Q117Q (CAA→CAG)	unknown	Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/25);  total (31/25)

GCACAGCAAGATATTAATGGCATTTCAATGATTAACTGCTTTTGTTCTATTAATGTTTCAGCCGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGG  >  1/103220‑103571                                                                                                            |                                                                                                                                                                                                                                                      tttaaagataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTaa                                                                                                       >  1:294302/25‑251 (MQ=255)      agataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCg                                                                                                  >  1:472056/20‑251 (MQ=255)       gataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGa                                                                                                 >  1:81005/19‑251 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATaaa                                                                                             >  1:258055/14‑251 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAgg                                                                                                    >  1:205766/14‑243 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAgg                                                                                                    >  1:230175/14‑243 (MQ=255)                gaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCaaagaa                                                                                                                <  1:149309/218‑1 (MQ=255)                gaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatcta                                                                                        >  1:114100/10‑251 (MQ=255)                 aatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATc                                                                                                                                                                      <  1:152083/164‑1 (MQ=255)                  atgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatt                                                                                      >  1:354224/8‑250 (MQ=255)                  atgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatt                                                                                      >  1:330223/8‑250 (MQ=255)                    gtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAagag                                                                                                            >  1:885458/6‑227 (MQ=255)                    gtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatttg                                                                                    >  1:368046/6‑248 (MQ=255)                     tatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTgg                                                                                   <  1:490273/247‑1 (MQ=255)                        atCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac                                                                                <  1:839838/250‑3 (MQ=255)                        atCAATGATTAACTGCTGTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac                                                                                <  1:455163/250‑3 (MQ=255)                            aTGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGaa                                                                            >  1:606283/1‑251 (MQ=255)                             tGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAAc                                                                           <  1:342471/251‑1 (MQ=255)                              gATTATCTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTgg                                                                                   <  1:552499/242‑1 (MQ=255)                              gATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTggg                                                                                                                                                                                   >  1:237619/1‑146 (MQ=255)                              gATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCaa                                                                                                                                                                    <  1:674060/161‑1 (MQ=255)                                      gCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAg                                                                   >  1:817999/1‑250 (MQ=255)                                      gCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGAGATCAGATATTAGTg                                                                                                                              >  1:339937/1‑191 (MQ=255)                                       cTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAAtt                                                                                                                                                                                                                      >  1:163529/1‑102 (MQ=255)                                       cTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATc                                                                                                                                                                      >  1:375408/1‑150 (MQ=255)                                             ttccaTTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATggg                                                           <  1:759208/247‑1 (MQ=255)                                               ccaTTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGgcg                                                                                                                                                                                                                                                    >  1:666097/3‑65 (MQ=255)                                               ccaTTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGtt                                                         >  1:444471/3‑251 (MQ=255)                                                     aTGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGAt                                                                                                                           <  1:775482/179‑1 (MQ=255)                                                     aTGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCg                                                    >  1:344243/1‑250 (MQ=255)                                                              cgggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAAt                                           <  1:155213/248‑1 (MQ=255)                                                              cgggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATc                                          <  1:379065/249‑1 (MQ=255)                                                               gggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCg                                                                                                                                                                                                                 <  1:839414/82‑1 (MQ=255)                                                                ggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGTAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCg                                                    <  1:437377/239‑1 (MQ=255)                                                                ggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAg                                                                                                                                       >  1:101264/1‑156 (MQ=255)                                                                ggCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCaa                                        >  1:382988/1‑251 (MQ=255)                                                                  cTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac                                                                                <  1:670789/209‑3 (MQ=255)                                                                  cTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGc                                                     >  1:266119/1‑236 (MQ=255)                                                                          gTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGt                                                                                                                               <  1:349927/154‑1 (MQ=255)                                                                          gTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGAt                                                                                                                           <  1:679385/158‑1 (MQ=255)                                                                          gTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATc                                                                                                                      <  1:732307/163‑1 (MQ=255)                                                                          gTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACggg                              >  1:881176/1‑251 (MQ=255)                                                                           tttCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATg                                                                                                                                                                  <  1:128107/118‑1 (MQ=255)                                                                            ttCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGGGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATggg                                                           >  1:451203/1‑220 (MQ=255)                                                                            ttCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATggg                                                           >  1:506601/1‑220 (MQ=255)                                                                                 tGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGc                       <  1:189867/251‑1 (MQ=255)                                                                                     ggCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCAt                   >  1:791780/1‑251 (MQ=255)                                                                                      gCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctattt                                                                                     <  1:206160/184‑3 (MQ=255)                                                                                      gCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAAc                                                                           >  1:766923/1‑194 (MQ=255)                                                                                      gCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGc                       <  1:180760/246‑1 (MQ=255)                                                                                       cGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCAtt                  <  1:309408/250‑1 (MQ=255)                                                                                         tAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGAt                                                                                                                           >  1:43628/1‑143 (MQ=255)                                                                                          aaCGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCg                                                                                                                                                                                                                 >  1:163282/1‑56 (MQ=255)                                                                                                     aCGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaaga    <  1:469540/250‑1 (MQ=37)                                                                                                      cGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATaaagaag  <  1:653321/251‑3 (MQ=37)                                                                                                        ggCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCg                                                    >  1:417633/1‑199 (MQ=255)                                                                                                            |                                                                                                                                                                                                                                                      GCACAGCAAGATATTAATGGCATTTCAATGATTAACTGCTTTTGTTCTATTAATGTTTCAGCCGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGG  >  1/103220‑103571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑