Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	103,282	C→G	A102A (GCC→GCG)	unknown →	Transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	103,282	0	C	G	100.0%	82.0 / NA	30	A102A (GCC→GCG)	unknown	Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (20/10);  total (20/10)

GCACAGCAAGATATTAATGGCATTTCAATGATTAACTGCTTTTGTTCTATTAATGTTTCAGCCGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCG  >  1/103220‑103521                                                               |                                                                                                                                                                                                                                                 tttaaagataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTaa                                                     >  1:294302/25‑251 (MQ=255)      agataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCg                                                >  1:472056/20‑251 (MQ=255)       gataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGa                                               >  1:81005/19‑251 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATaaa                                           >  1:258055/14‑251 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAgg                                                  >  1:205766/14‑243 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAgg                                                  >  1:230175/14‑243 (MQ=255)                gaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCaaagaa                                                              <  1:149309/218‑1 (MQ=255)                gaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatcta                                      >  1:114100/10‑251 (MQ=255)                 aatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATc                                                                                                                    <  1:152083/164‑1 (MQ=255)                  atgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatt                                    >  1:330223/8‑250 (MQ=255)                  atgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatt                                    >  1:354224/8‑250 (MQ=255)                    gtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAagag                                                          >  1:885458/6‑227 (MQ=255)                    gtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatttg                                  >  1:368046/6‑248 (MQ=255)                     tatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTgg                                 <  1:490273/247‑1 (MQ=255)                        atCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac                              <  1:839838/250‑3 (MQ=255)                        atCAATGATTAACTGCTGTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac                              <  1:455163/250‑3 (MQ=255)                            aTGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGaa                          >  1:606283/1‑251 (MQ=255)                             tGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAAc                         <  1:342471/251‑1 (MQ=255)                              gATTATCTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTgg                                 <  1:552499/242‑1 (MQ=255)                              gATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTggg                                                                                                                                 >  1:237619/1‑146 (MQ=255)                              gATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCaa                                                                                                                  <  1:674060/161‑1 (MQ=255)                                      gCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAg                 >  1:817999/1‑250 (MQ=255)                                      gCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGAGATCAGATATTAGTg                                                                            >  1:339937/1‑191 (MQ=255)                                       cTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAAtt                                                                                                                                                                    >  1:163529/1‑102 (MQ=255)                                       cTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATc                                                                                                                    >  1:375408/1‑150 (MQ=255)                                             ttccaTTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATggg         <  1:759208/247‑1 (MQ=255)                                               ccaTTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGgcg                                                                                                                                                                                                  >  1:666097/3‑65 (MQ=255)                                               ccaTTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGtt       >  1:444471/3‑251 (MQ=255)                                                     aTGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGAt                                                                         <  1:775482/179‑1 (MQ=255)                                                     aTGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCg  >  1:344243/1‑250 (MQ=255)                                                               |                                                                                                                                                                                                                                                 GCACAGCAAGATATTAATGGCATTTCAATGATTAACTGCTTTTGTTCTATTAATGTTTCAGCCGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCG  >  1/103220‑103521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑