Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,079,749	G→A	G901E (GGG→GAG)	unknown →	Isoleucyl‑tRNA synthetase (EC 6.1.1.5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,079,749	0	G	A	100.0%	165.1 / NA	57	G901E (GGG→GAG)	unknown	Isoleucyl‑tRNA synthetase (EC 6.1.1.5)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/29);  total (28/29)

TTCCGAAGTAAACCGCGTGTTGGAAATCGCCCGTAACGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGGGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACGAACAAACCAAAATCCGGGCTCTCATTTTTGTGG  >  1/2079504‑2079988                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ttCCGAAGTAAACCGCGTGTTGGAAATCGCCCGTAACGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGaaa                                                                                                                                                                                                                                             <  1:39026/250‑1 (MQ=255) ttCCGAAGTAAACCGCGTGTTGGAAATCGCCCGTAACGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGaaa                                                                                                                                                                                                                                             <  1:175844/250‑1 (MQ=255) ttCCGAAGTAAACCGCGTGTTGGAAATCGCCCGTAACGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGaaa                                                                                                                                                                                                                                             <  1:794127/250‑1 (MQ=255)                  tgtTGGAAATCGCCCGTAACGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCa                                                                                                                                                                                                                            <  1:25074/250‑1 (MQ=255)                             gCCCGTAACGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTgg                                                                                                                                                                                                                              <  1:29188/237‑1 (MQ=255)                                    aCGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCatt                                                                                                                                                                                                                          <  1:392743/234‑1 (MQ=255)                                    aCGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCgg                                                                                                                                                                                                          >  1:810473/1‑250 (MQ=255)                                     cGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCg                                                                                                                                                                                                        >  1:810763/1‑251 (MQ=255)                                         cacgaattCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGACGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCaa                                                                                                                                                                                                    >  1:207927/8‑251 (MQ=255)                                         aaaGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCaa                                                                                                                                                                                                    >  1:222170/1‑251 (MQ=255)                                                    cgcCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATc                                                                                                                                                                                         <  1:486774/251‑1 (MQ=255)                                                          tttGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGcc                                                                                                                                                                                   <  1:320836/251‑1 (MQ=255)                                                                     aaGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGt                                                                                                                                                                        <  1:684885/251‑1 (MQ=255)                                                                        tGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCACCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTg                                                                                                                                                                      >  1:831817/1‑250 (MQ=255)                                                                             gTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTggg                                                                                                                                                                >  1:390333/1‑250 (MQ=255)                                                                             gTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGa                                                                                                                                                                >  1:353801/1‑251 (MQ=255)                                                                               ttACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGaaa                                                                                                                                                              <  1:379668/251‑1 (MQ=255)                                                                                         gacgaATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACg                                                                                                                                                           >  1:867058/1‑244 (MQ=255)                                                                                         gacgaATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAgg                                                                                                                                                    >  1:492595/1‑251 (MQ=255)                                                                                         gacgaATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAgg                                                                                                                                                    >  1:152713/1‑251 (MQ=255)                                                                                         gacgaATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAgg                                                                                                                                                    >  1:123438/1‑251 (MQ=255)                                                                                         gacgaATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCATTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAgg                                                                                                                                                    >  1:452029/1‑251 (MQ=255)                                                                                             aaTATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAAc                                                                                                                                                <  1:103048/251‑1 (MQ=255)                                                                                                                                    gTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATaaa                                                                                                         >  1:214052/1‑251 (MQ=255)                                                                                                                                         gtgtTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTc                                                                                                                                                                                                                      <  1:519481/137‑1 (MQ=255)                                                                                                                                         gtgtTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAg                                                                                                    >  1:759095/1‑251 (MQ=255)                                                                                                                                           gtTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGTTGCCAATAAAAAAAGTg                                                                                                  >  1:4635/1‑251 (MQ=255)                                                                                                                                              gATTACCTCTAAAGCGGAAGTCAAACCATTGACAGGCAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGcc                                                                                                                                                                                   <  1:793046/167‑1 (MQ=255)                                                                                                                                                   ccTCTAAAGCGGAAGTCAAACTATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTaaa                                                                                          >  1:641386/1‑251 (MQ=255)                                                                                                                                                   ccTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACAttt                                                                                                                               >  1:516182/1‑214 (MQ=255)                                                                                                                                                   ccTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTaaa                                                                                          >  1:656094/1‑251 (MQ=255)                                                                                                                                                   ccTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGAAACGGCGAAAACCGACATTTCGCTTAATTTGATGCCAATAAAAAAAGTGCGGTTaaa                                                                                          >  1:805408/1‑251 (MQ=255)                                                                                                                                                         aaGCGGAAGTCAAACCATTGACAGACAACCCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGAc                                                                                    <  1:568245/251‑1 (MQ=255)                                                                                                                                                                      aCCATTGACAGACAATCCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTttatta                                                                       <  1:6720/251‑1 (MQ=255)                                                                                                                                                                      aCCATTGACAGACAACCCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTttatta                                                                       <  1:422442/251‑1 (MQ=255)                                                                                                                                                                      aCCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTttatta                                                                       >  1:675697/1‑251 (MQ=255)                                                                                                                                                                       ccATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTtattat                                                                      >  1:296675/1‑251 (MQ=255)                                                                                                                                                                                gacaAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAAt                                                             >  1:765/1‑251 (MQ=255)                                                                                                                                                                                 acaAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATc                                                            <  1:594112/251‑1 (MQ=255)                                                                                                                                                                                  caAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCa                                                           <  1:807463/251‑1 (MQ=255)                                                                                                                                                                                          ccGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAgatgat                                                    <  1:535298/250‑1 (MQ=255)                                                                                                                                                                                          ccGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAgatgat                                                    <  1:742010/250‑1 (MQ=255)                                                                                                                                                                                               gtgGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGaaa                                                                                                                                                              <  1:103679/139‑1 (MQ=255)                                                                                                                                                                                                tgGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGa                                             <  1:461995/251‑1 (MQ=255)                                                                                                                                                                                                 ggCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGaa                                            >  1:787637/1‑251 (MQ=255)                                                                                                                                                                                                         gCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGa                                     <  1:113594/250‑1 (MQ=255)                                                                                                                                                                                                          cGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACg                                   <  1:595219/251‑1 (MQ=255)                                                                                                                                                                                                          cGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACg                                   <  1:201461/251‑1 (MQ=255)                                                                                                                                                                                                            aGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAgatg                                                      <  1:319440/230‑1 (MQ=255)                                                                                                                                                                                                              ttGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGTGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACGAAc                                <  1:454147/250‑1 (MQ=255)                                                                                                                                                                                                              ttGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACGAAc                                <  1:672256/250‑1 (MQ=255)                                                                                                                                                                                                                   aGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACGAACAAACCa                          >  1:339976/1‑251 (MQ=255)                                                                                                                                                                                                                        tCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTttatta                                                                       >  1:20007/1‑201 (MQ=255)                                                                                                                                                                                                                        tCGCCGTGAGCATCGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACGAACAAACCAAAATc                     <  1:243986/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGCCCGTTCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGGAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACGAACAAACCAAAATCCGGGCTCTCAttt        >  1:178401/1‑251 (MQ=255)                                                                                                                                                                                                                                           ttCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACGAACAAACCAAAATCCGGGCTCTCATTTTTGTgg  >  1:124058/1‑251 (MQ=255)                                                                                                                                                                                                                                           ttCCAACGGCGAGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACGAACAAACCAAAATCCGGGCTCTCATTTTTGTgg  >  1:884013/1‑251 (MQ=255)                                                                                                                                                                                                                                                  ggcgaGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAgatg                                                      >  1:111179/1‑192 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TTCCGAAGTAAACCGCGTGTTGGAAATCGCCCGTAACGATAAAGTGATCGGCGCCGGTTTGGAAGCGGAAGTGACCGTTTACACCAACGACGAATATCGTGCGTTATTGGAAAAACTCGGCGACGAACTCCGTTTCGTGTTGATTACCTCTAAAGCGGAAGTCAAACCATTGACAGACAAACCTGCCGATGTGGCGGAGGGCGAGTTGGAAGGCATCGCCGTGAGCATCGCCCGTTCCAACGGCGGGAAATGCCCGCGTTGCTGGCATTATTCCGACAAAATCGGCGTCAACCCGCAACATCCGAGCCTTTGTCCGCGTTGCGTGGAAAACGTGGCAGGTAACGGCGAAATCCGACATTTCGCTTAATTTGCTGCCAATAAAAAAAGTGCGGTTAAAATCGACCGTACTTTTATTATTTTTATAATCAAGATGATTTAACGAACTTATGACGAACAAACCAAAATCCGGGCTCTCATTTTTGTGG  >  1/2079504‑2079988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑