Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,077,734	T→C	V229V (GTT→GTC)	unknown →	Isoleucyl‑tRNA synthetase (EC 6.1.1.5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,077,734	0	T	C	100.0%	195.2 / NA	70	V229V (GTT→GTC)	unknown	Isoleucyl‑tRNA synthetase (EC 6.1.1.5)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/34);  total (36/34)

ATTGGGATAATCCGTACCTCACCATGAATTTCAACACCGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTTGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGATCTTGAGTTATTC  >  1/2077493‑2077962                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     aTTGGGATAATCCGTACCTCACCATGAATTTCAACACCGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGc                                                                                                                                                                                                                             <  1:462045/251‑1 (MQ=255)    gggATAATCCGTACCTCACCATGAATTTCAACACCGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAg                                                                                                                                                                                                                           >  1:582110/1‑250 (MQ=255)          aTCCGTACCTCACCATGAATTTCAACACCGAAGCCAACATTGTTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAg                                                                                                                                                                                                                    >  1:111423/1‑251 (MQ=255)          aTCCGTACCTCACCATGAATTTCAACACCGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGc                                                                                                                                                                                                                             <  1:216653/242‑1 (MQ=255)          aTCCGTACCTCACCATGAATTTCAACACCGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAg                                                                                                                                                                                                                    >  1:148897/1‑251 (MQ=255)              gTACCTCACCATGAATTTCAACACCGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGt                                                                                                                                                                                                                >  1:206707/1‑251 (MQ=255)                        aTGAATTTCAACACCGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGAt                                                                                                                                                                                                      >  1:605790/1‑251 (MQ=255)                             tttCAACACCGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCAGCCGTGATTTGGa                                                                                                                                                                                                 >  1:82745/1‑251 (MQ=255)                                     ccGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAAc                                                                                                                                                                                          >  1:500478/1‑250 (MQ=255)                                             aaCATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGAc                                                                                                                                                                                 <  1:811029/251‑1 (MQ=255)                                             aaCATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGAc                                                                                                                                                                                 <  1:695500/251‑1 (MQ=255)                                                 ttattCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGAc                                                                                                                                                                                 >  1:810888/1‑247 (MQ=255)                                                  tattCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGc                                                                                                                                                                            <  1:421152/251‑1 (MQ=255)                                                        cACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGAcc                                                                                                                                                                                >  1:484540/1‑241 (MQ=255)                                                            ttGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCg                                                                                                                                                                  <  1:653013/251‑1 (MQ=255)                                                                     gTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGc                                                                                                                                                                                                                             >  1:134332/1‑183 (MQ=255)                                                                     gTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTAttt                                                                                                                                                          >  1:872232/1‑250 (MQ=255)                                                                      tGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTc                                                                                                                                                         <  1:364227/250‑1 (MQ=255)                                                                        aTCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCg                                                                                                                                                       <  1:671084/250‑1 (MQ=255)                                                                                        tatataAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTg                                                                                                                                                                                                                 >  1:519773/1‑176 (MQ=255)                                                                                         atataAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGc                                                                                                                                                                                                                             >  1:451008/1‑163 (MQ=255)                                                                                         atataAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGAc                                                                                                                                                                                                <  1:205470/192‑1 (MQ=255)                                                                                          tataAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCg                                                                                                                                                       <  1:406469/232‑1 (MQ=255)                                                                                             aaaGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCa                                                                                                                                 >  1:425018/1‑251 (MQ=255)                                                                                                         ccGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAAtt                                                                                                                     >  1:676541/1‑251 (MQ=255)                                                                                                         ccGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTCGTccaaaccgtgattcggcgc                                                                                                                                                                                             <  1:52571/179‑18 (MQ=255)                                                                                                            gTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAgg                                                                                                                  >  1:326956/1‑251 (MQ=255)                                                                                                               cATTGGTGTTTGGATTGTGTCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGcga                                                                                                               <  1:335165/251‑1 (MQ=255)                                                                                                                    gtgtTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAg                                                                                                           <  1:14693/250‑1 (MQ=255)                                                                                                                    gtgtTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAg                                                                                                           <  1:102308/250‑1 (MQ=255)                                                                                                                        ttGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGcgcgg                                                                                                      >  1:233864/1‑250 (MQ=255)                                                                                                                                                    cggaagTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAg                                                                            <  1:515649/249‑1 (MQ=255)                                                                                                                                                      gaagTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAAAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACAt                                                                        >  1:658156/1‑251 (MQ=255)                                                                                                                                                         gTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGt                                                                     >  1:758852/1‑251 (MQ=255)                                                                                                                                                           ggAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCg                                                                                                                                                       >  1:641290/1‑167 (MQ=255)                                                                                                                                                            gAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGaa                                                                  >  1:294542/1‑251 (MQ=255)                                                                                                                                                            gAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGa                                                                   <  1:85354/250‑1 (MQ=255)                                                                                                                                                              atatGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAg                                                                                                                                <  1:578149/187‑1 (MQ=255)                                                                                                                                                              atatGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGc                                                                                                                 >  1:103592/1‑202 (MQ=255)                                                                                                                                                              atatGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAgg                                                                >  1:311918/1‑251 (MQ=255)                                                                                                                                                               tatGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGc                                                                                                                                                               >  1:307834/1‑155 (MQ=255)                                                                                                                                                               tatGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAg                                                                                                           >  1:20944/1‑207 (MQ=255)                                                                                                                                                               tatGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGa                                                                   >  1:282257/1‑247 (MQ=255)                                                                                                                                                                     gACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTgg                                                         >  1:71694/1‑251 (MQ=255)                                                                                                                                                                         gtgtgtCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAGTATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTg                                                     >  1:452042/1‑251 (MQ=255)                                                                                                                                                                         gtgtgtCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGAc                                                                          >  1:720548/1‑230 (MQ=255)                                                                                                                                                                          tgtgtCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTgg                                                    <  1:129074/251‑1 (MQ=255)                                                                                                                                                                              tCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGaaa                                                 <  1:323629/250‑1 (MQ=255)                                                                                                                                                                                   gTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGt                                                                     <  1:690470/225‑1 (MQ=255)                                                                                                                                                                                     ccATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGaa                                         >  1:627530/1‑251 (MQ=255)                                                                                                                                                                                        tttATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAg                                                                                                                                <  1:899824/161‑1 (MQ=255)                                                                                                                                                                                        tttATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGc                                                                                             <  1:580458/196‑1 (MQ=255)                                                                                                                                                                                        tttATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGc                                                               <  1:329374/226‑1 (MQ=255)                                                                                                                                                                                        tttATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAAt                                       >  1:130051/1‑250 (MQ=255)                                                                                                                                                                                        tttACGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAg                                                                                                           <  1:480495/182‑1 (MQ=255)                                                                                                                                                                                             gTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAgg                                 >  1:848451/1‑251 (MQ=255)                                                                                                                                                                                              tACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGt                                <  1:517982/251‑1 (MQ=255)                                                                                                                                                                                              tACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGt                                <  1:275073/251‑1 (MQ=255)                                                                                                                                                                                               aCGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAAc                                                                                                                                                                    >  1:160346/1‑118 (MQ=255)                                                                                                                                                                                                             aaaGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGc                                                                                                                         >  1:322673/1‑147 (MQ=255)                                                                                                                                                                                                                 tGCGGTGGAAGTTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGa               <  1:51361/249‑1 (MQ=255)                                                                                                                                                                                                                 tGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGa               <  1:791989/249‑1 (MQ=255)                                                                                                                                                                                                                 tGCGGTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGa               <  1:523778/249‑1 (MQ=255)                                                                                                                                                                                                                     gTGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGATCTTGa         >  1:770876/1‑251 (MQ=255)                                                                                                                                                                                                                      tGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGATCTTGAg        <  1:593741/251‑1 (MQ=255)                                                                                                                                                                                                                      tGGAAATTGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGATCTTGAg        <  1:309261/251‑1 (MQ=255)                                                                                                                                                                                                                            ttGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGATCTTGAGTTATTc  <  1:260179/251‑1 (MQ=255)                                                                                                                                                                                                                            ttGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGATCTTGAGTTATTc  <  1:773453/251‑1 (MQ=255)                                                                                                                                                                                                                            ttGAAGGTAAATTTAACGCCGTCGGCAAAGGCAGAGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGATCTTGAGTTATTc  <  1:849928/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTTTAACGCCGTCGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGATCTTGAGt       >  1:880692/1‑236 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     ATTGGGATAATCCGTACCTCACCATGAATTTCAACACCGAAGCCAACATTATTCGCACTTTGGGCAAAGTGATCGCCAACGGGCATTTATATAAAGGCTCCAAACCGGTGCATTGGTGTTTGGATTGTGGCTCCTCTTTGGCGGAAGCGGAAGTGGAATATGAAGACCGTGTGTCGCCGTCCATTTATGTACGTTTCCCGGCAGAAAGTGCGGTGGAAATTGAAGGTAAATTTAACGCCGTTGGCAAAGGCAGCGGCAAGTTGTCCGCCGTGATTTGGACCACAACCCCTTGGACCATGCCGTCCAACCGTGCTATTTCCGTGAATGCCGAGCTGGAATATCAGCTTGTGCAATTAGGCGACGAGCGCGTGATTTTAGCTGCCGATTTGGTGGAAGACATGGTGAAGGCGGTTGGCGTGGAAAAGGTGGAAATCTTAGGTTCCGCAAAAGGTAAAGATCTTGAGTTATTC  >  1/2077493‑2077962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑