Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	103,267	T→C	S97S (TCT→TCC)	unknown →	Transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	103,267	0	T	C	100.0%	70.2 / NA	25	S97S (TCT→TCC)	unknown	Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/8);  total (17/8)

GCACAGCAAGATATTAATGGCATTTCAATGATTAACTGCTTTTGTTCTATTAATGTTTCAGCCGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAG  >  1/103220‑103506                                                |                                                                                                                                                                                                                                                 tttaaagataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTaa                                      >  1:294302/25‑251 (MQ=255)      agataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCg                                 >  1:472056/20‑251 (MQ=255)       gataatatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGa                                >  1:81005/19‑251 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCATAAAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATaaa                            >  1:258055/14‑251 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAgg                                   >  1:205766/14‑243 (MQ=255)            tatggaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAgg                                   >  1:230175/14‑243 (MQ=255)                gaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCaaagaa                                               <  1:149309/218‑1 (MQ=255)                gaatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatcta                       >  1:114100/10‑251 (MQ=255)                 aatgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATc                                                                                                     <  1:152083/164‑1 (MQ=255)                  atgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatt                     >  1:354224/8‑250 (MQ=255)                  atgtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatt                     >  1:330223/8‑250 (MQ=255)                    gtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAagag                                           >  1:885458/6‑227 (MQ=255)                    gtatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAatctatttg                   >  1:368046/6‑248 (MQ=255)                     tatatCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTgg                  <  1:490273/247‑1 (MQ=255)                        atCAATGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac               <  1:839838/250‑3 (MQ=255)                        atCAATGATTAACTGCTGTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGtac               <  1:455163/250‑3 (MQ=255)                            aTGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGaa           >  1:606283/1‑251 (MQ=255)                             tGATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAAc          <  1:342471/251‑1 (MQ=255)                              gATTATCTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTgg                  <  1:552499/242‑1 (MQ=255)                              gATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTggg                                                                                                                  >  1:237619/1‑146 (MQ=255)                              gATTAACTGCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCaa                                                                                                   <  1:674060/161‑1 (MQ=255)                                      gCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAg  >  1:817999/1‑250 (MQ=255)                                      gCTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGAGATCAGATATTAGTg                                                             >  1:339937/1‑191 (MQ=255)                                       cTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAAtt                                                                                                                                                     >  1:163529/1‑102 (MQ=255)                                       cTTTTGTTCCATTAATGTTTCAGCGGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAGGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCCGTTTTTTGGGCGGACGGCGTATc                                                                                                     >  1:375408/1‑150 (MQ=255)                                                |                                                                                                                                                                                                                                                 GCACAGCAAGATATTAATGGCATTTCAATGATTAACTGCTTTTGTTCTATTAATGTTTCAGCCGGCTATGGCAGTTTCAATGAGGGCGTAACGCAACCGGACGGGCAAGCGCCATACTCGGAAACGTTATTGCAT‑AAATTAGGCGTAAAAGCGCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAG  >  1/103220‑103506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑