Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	102,751	G→A	S4L (TCA→TTA)	unknown ←	DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	102,751	0	G	A	100.0%	70.9 / NA	26	S4L (TCA→TTA)	unknown	DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/18);  total (8/18)

GCTTGCGATTACGTTCAATCCGCTCAAACGCCCGCGCTGCCCAAATCTGTTCAGGGGGAATGCCGACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTGGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGTAATATTTGATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAGTGTATTAAACGGTAAAAGTTTACATT  >  1/102506‑102818                                                                                                                                                                                                                                                      |                                                                    gCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATac                                                                 <  1:820011/250‑1 (MQ=255)  cTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATacac                                                               <  1:268667/251‑1 (MQ=255)          tACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAAc                                                       <  1:838453/251‑1 (MQ=255)           aCGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAAc                                                       <  1:354921/250‑1 (MQ=255)            cGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTa                                                     <  1:296991/251‑1 (MQ=255)            cGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTa                                                     <  1:659063/251‑1 (MQ=255)                          aagcGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTAtg                                       <  1:101033/248‑1 (MQ=255)                          aagcGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTAtg                                       <  1:232557/248‑1 (MQ=255)                          aagcGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTAtg                                       <  1:459791/248‑1 (MQ=255)                             cGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATgtgt                                    >  1:329533/1‑251 (MQ=255)                                       gCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAgcg                          >  1:671967/1‑249 (MQ=255)                                        cccAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATac                                                                 <  1:735130/211‑1 (MQ=255)                                            aatttGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAGCGTATTa                     >  1:19892/5‑251 (MQ=255)                                                             cgccaaCAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAGCGTATTAAAACGTaaaacttaatt    <  1:501419/246‑8 (MQ=255)                                                               ccaaCAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAGCGTATTAAAACGTaaaacttaattat  <  1:570454/248‑10 (MQ=255)                                                                   cAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATGTGTTTCATg                              <  1:837633/219‑1 (MQ=255)                                                                     gCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTa                                                     <  1:652689/194‑1 (MQ=255)                                                                                                      ggTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTa                                                     >  1:812134/1‑161 (MQ=255)                                                                                                                                   gtAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATac                                                                 >  1:248815/1‑120 (MQ=255)                                                                                                                                     aaCTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAGCGTATTAAAACGTaaaacttaatta   >  1:703369/1‑172 (MQ=255)                                                                                                                                                          cTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAAc                                                       <  1:821918/107‑1 (MQ=255)                                                                                                                                                          cTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAg                            <  1:495976/134‑1 (MQ=255)                                                                                                                                                                 aCGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTAtg                                       <  1:818338/116‑1 (MQ=255)                                                                                                                                                                                    tttCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAGCGTATTAAAACGTaaaacttaatta   <  1:518455/133‑9 (MQ=255)                                                                                                                                                                                                                 aTGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAGCGTATTAAAACGTaaaact         >  1:249999/1‑96 (MQ=255)                                                                                                                                                                                                                    ccAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATac                                                                 >  1:212667/1‑39 (MQ=255)                                                                                                                                                                                                                                                      |                                                                    GCTTGCGATTACGTTCAATCCGCTCAAACGCCCGCGCTGCCCAAATCTGTTCAGGGGGAATGCCGACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTGGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGTAATATTTGATACACTCATTAACTACATTCCTTTTTATGTGTTTCATGAGTGTATTAAACGGTAAAAGTTTACATT  >  1/102506‑102818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑