Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,065,711	C→T	G94G (GGC→GGT)	unknown →	UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase (EC 6.3.2.‑)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,065,711	0	C	T	100.0%	191.8 / NA	66	G94G (GGC→GGT)	unknown	UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase (EC 6.3.2.‑)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/33);  total (33/33)

GCGGCATTGCGATGATCGCCAAGCAAATGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGCCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGTTTTTTGATAAACGAT  >  1/2065479‑2065949                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               gcggcATTGCGATGATCGCCAAGCAAATGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGa                                                                                                                                                                                                                               <  1:156813/250‑1 (MQ=255)    gcATTGCGATGATCGCCAAGCAAATGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCAt                                                                                                                                                                                                                           >  1:579186/1‑251 (MQ=255)      aTTGCGATGATCGCCAAGCAAATGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCAttt                                                                                                                                                                                                                         <  1:298031/251‑1 (MQ=255)        tGCGATGATCGCCAAGCAAATGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATttat                                                                                                                                                                                                                       <  1:840444/251‑1 (MQ=255)        tGCGATGATCGCCAAGCAAATGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATttat                                                                                                                                                                                                                       <  1:392289/251‑1 (MQ=255)               atCGCCAAGCAAATGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCAAACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGa                                                                                                                                                                                                                <  1:69216/251‑1 (MQ=255)                       gCAAATGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTggg                                                                                                                                                                                                        >  1:184621/1‑251 (MQ=255)                       gCAAATGGGCTATCATGTAACAGGCTCCGACACAAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACg                                                                                                                                                                                                                                <  1:234139/227‑1 (MQ=255)                         aaaTGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGtt                                                                                                                                                                                                      <  1:648814/251‑1 (MQ=255)                                  aTCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACg                                                                                                                                                                                                                                <  1:143879/216‑1 (MQ=255)                                  aTCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGtt                                                                                                                                                                                             >  1:886804/1‑251 (MQ=255)                                  aTCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGtt                                                                                                                                                                                             >  1:764236/1‑251 (MQ=255)                                  aTCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGtt                                                                                                                                                                                             >  1:729496/1‑251 (MQ=255)                                   tCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGAt                                                                                                                                                                                                                              <  1:127194/217‑1 (MQ=255)                                   tCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGttt                                                                                                                                                                                            >  1:357973/1‑251 (MQ=255)                                       gTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCgg                                                                                                                                                                                        >  1:163109/1‑251 (MQ=255)                                           cAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGcaca                                                                                                                                                                                    >  1:705745/1‑251 (MQ=255)                                             ggCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATttat                                                                                                                                                                                                                       <  1:69776/214‑1 (MQ=255)                                              gCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGcacac                                                                                                                                                                                   <  1:418715/249‑1 (MQ=255)                                                    acacCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAAt                                                                                                                                                                                                                                         <  1:664164/189‑1 (MQ=255)                                                      acCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCa                                                                                                                                                                                                                            <  1:19383/200‑1 (MQ=255)                                                           cGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGa                                                                                                                                                                    >  1:779538/1‑251 (MQ=255)                                                                     ccaATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTCCACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGcac                                                                                                                                                                                     <  1:758919/224‑1 (MQ=255)                                                                     ccaATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTAc                                                                                                                                                                                                                    <  1:362071/193‑1 (MQ=255)                                                                            gCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATg                                                                                                                                                                                                                                        <  1:269038/166‑1 (MQ=255)                                                                                              aTCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACaaaa                                                                                                                                  >  1:47169/1‑250 (MQ=255)                                                                                                      gAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCa                                                                                                                         >  1:859417/1‑251 (MQ=255)                                                                                                                    cTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATttat                                                                                                                                                                                                                       >  1:76719/1‑143 (MQ=255)                                                                                                                                ccaGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTg                                                                                               <  1:428011/251‑1 (MQ=255)                                                                                                                                   gTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAg                                                                                            >  1:760849/1‑251 (MQ=255)                                                                                                                                             gcgcCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCgg                                                                                  >  1:365354/1‑251 (MQ=255)                                                                                                                                                       tGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCa                                                                        >  1:595232/1‑251 (MQ=255)                                                                                                                                                       tGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCa                                                                        <  1:150660/251‑1 (MQ=255)                                                                                                                                                             tCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTa                                                                  >  1:328526/1‑251 (MQ=255)                                                                                                                                                             tCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTa                                                                  >  1:857303/1‑251 (MQ=255)                                                                                                                                                                 gggAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAggg                                                               >  1:816112/1‑250 (MQ=255)                                                                                                                                                                  ggAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGc                                                              <  1:210771/250‑1 (MQ=255)                                                                                                                                                                  ggAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGc                                                              <  1:147740/250‑1 (MQ=255)                                                                                                                                                                  ggAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGc                                                              <  1:78060/250‑1 (MQ=255)                                                                                                                                                                   gAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCaa                                                            >  1:3737/1‑251 (MQ=255)                                                                                                                                                                      aTGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGc                                                         <  1:815088/251‑1 (MQ=255)                                                                                                                                                                        gCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAGCATTCCTTACACGTCCGGTCCGCAATGGTTGCACGa                                                                                                                                                                                                                               <  1:41507/83‑1 (MQ=255)                                                                                                                                                                                   cgGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTa                                            >  1:823449/1‑251 (MQ=255)                                                                                                                                                                                   cgGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTa                                            >  1:108973/1‑251 (MQ=255)                                                                                                                                                                                         tCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAg                                      >  1:290011/1‑251 (MQ=255)                                                                                                                                                                                                   gAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATttat                                                                                                                                                                                                                       >  1:71226/1‑64 (MQ=255)                                                                                                                                                                                                   gAGTATATGTTAGCAAATAACATTCATTACACGACCGGTCCGAAATGGTTGCACGAATATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGTAAAACCACGACCACCGGTATGCTCACTTGGATTTTGGTATAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTAATTCCGCACGCTACGGGCATAGTCCGAATTTTGTTATTGAAGCGGATGAata                            >  1:283975/1‑251 (MQ=255)                                                                                                                                                                                                      tataTGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAatac                           <  1:138982/249‑1 (MQ=255)                                                                                                                                                                                                      tataTGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAatac                           <  1:849789/249‑1 (MQ=255)                                                                                                                                                                                                      tataTGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAatac                           <  1:665371/249‑1 (MQ=255)                                                                                                                                                                                                      tataTGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAatac                           <  1:587760/249‑1 (MQ=255)                                                                                                                                                                                                       atatGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAatacgat                        <  1:527225/251‑1 (MQ=255)                                                                                                                                                                                                       atatGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAatacgat                        <  1:20770/251‑1 (MQ=255)                                                                                                                                                                                                         atGTTAGCAAATAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCAGTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAGCCGGGCTTTTTAATTGGCGGTATTGCAGGCAAGTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAg                                      <  1:671180/235‑1 (MQ=255)                                                                                                                                                                                                                  aaTAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGttttt             >  1:770180/1‑251 (MQ=255)                                                                                                                                                                                                                   aTAACATTCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGtttttt            >  1:87735/1‑251 (MQ=255)                                                                                                                                                                                                                          tCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTaa                                                                                                           >  1:825485/1‑149 (MQ=255)                                                                                                                                                                                                                          tCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAggg                                                               >  1:372186/1‑193 (MQ=255)                                                                                                                                                                                                                          tCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTAtt                                                  >  1:431919/1‑206 (MQ=255)                                                                                                                                                                                                                          tCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGTTTTTTGATaaa      >  1:862953/1‑250 (MQ=255)                                                                                                                                                                                                                          tCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGTTTTTTGATAAAc     <  1:791077/251‑1 (MQ=255)                                                                                                                                                                                                                          tCCTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGTTTTTTGATAAAc     <  1:584792/251‑1 (MQ=255)                                                                                                                                                                                                                           ccTTACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGTTTTTTGATAAACg    >  1:886595/1‑251 (MQ=255)                                                                                                                                                                                                                             ttACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAggg                                                               <  1:5264/190‑1 (MQ=255)                                                                                                                                                                                                                             ttACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGTTTTTTGATAAACGAt  >  1:120751/1‑251 (MQ=255)                                                                                                                                                                                                                              tACACGTCCGGTCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGTTTTTTGATAAACGAt  >  1:472677/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               GCGGCATTGCGATGATCGCCAAGCAAATGGGCTATCATGTAACAGGCTCCGACACCAACGTTTACCCACCAATGAGCACTTTTCTTGCCGAAAATCACATTGAAATCATACCGCACTTTGAGCCAAGCCAGTTACAACCAGCGCCGGATATGGTCATCGTGGGAAATGCCATGAAACGCGGCAATCCCTGCATCGAGTATATGTTAGCAAATAACATTCCTTACACGTCCGGCCCGCAATGGTTGCACGATCATTTATTACGGGATCGCTGGGTTTTGGCAGTTTCCGGCACACACGGCAAAACCACGACCACCGGCATGCTAACTTGGATTTTGGAACAAAACGGTTTGCAACCGGGCTTTTTAATTGGCGGTATTGCAGGCAATTTCGGTATTTCCGCACGCTTAGGGCAAAGCCCGTATTTTGTTATTGAAGCGGATGAATACGATACGGCGTTTTTTGATAAACGAT  >  1/2065479‑2065949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑