Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,048,052	A→G	G39G (GGT→GGC)	unknown ←	Lysine decarboxylase family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,048,052	0	A	G	100.0%	181.6 / NA	64	G39G (GGT→GGC)	unknown	Lysine decarboxylase family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/33);  total (31/33)

CCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGACAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCACCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTCCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAAT  >  1/2047811‑2048298                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       cccAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATaa                                                                                                                                                                                                                                               >  1:649183/1‑251 (MQ=255)   aaaaaaTAGACTCCGCTATTTTCTCCCAGCGTCCCCGCCCCCCCCGGAAAAGAAATAAAAGCATCGGCTAATGCAAGCTTTGTGGTTTTC‑CGCCCCGACAGGGAACCCAAAAGGAAAATTCCGGTACACCCTG‑GGTGTACCAATC‑CCCGCGCCTGAAAAAATTCAGGGACCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAAc                                                                                                                                                                                                                                             <  1:455127/247‑1 (MQ=255)     aGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTa                                                                                                                                                                                                                                           >  1:6126/1‑251 (MQ=255)     aGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTa                                                                                                                                                                                                                                           >  1:276226/1‑251 (MQ=255)      gAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTaa                                                                                                                                                                                                                                          >  1:119559/1‑251 (MQ=255)        atagcctccGCTGATTTCTCCCGGCGGGCCCGGCCCCCCGGGCAAAGAAATACAGGCACCGGCTAATGCCAGCATTTTGGCTTTC‑CGCCCCGACATGAAACCCAAAATAATATTTTCGGAACACCCTG‑GGTGTCCCATTT‑CCCGCGCCTGAAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACg                                                                                                                                                                                                                                        <  1:874748/243‑1 (MQ=255)                cGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCTGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATACACTAACGTATGTCGa                                                                                                                                                                                                                                >  1:415383/1‑251 (MQ=255)                  ctgtttttttcCAGCGTGCCCGCCCCCCCGGGCAAACAAATAAAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCAACATGGAACCCACAATGATAATTTCGGTACACCCTT‑GGTGTACCATTC‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATg                                                                                                                                                                                                                              <  1:448512/243‑1 (MQ=255)                         cttcCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGt                                                                                                                                                                                                                                   >  1:739367/1‑239 (MQ=255)                                    cccGCCCCACCCGGAAAAGCAATACAAGCATGGGCAAAGGCCAGCATTTTGGCTTTT‑CCCGCCGACATGGAATCCACAATAAAATTTTCGGTACACCCTT‑GGTGTCCAAATC‑CCCGCGCCGGAAAAAATTCAGGGATCACGCCAATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGcc                                                                                                                                                                                                            <  1:751917/251‑1 (MQ=255)                                        ccccccccggaaaaGCAATACAAGCACCGGTTAATCCCAGC‑TTTTGGGTTTTCCGCCCCGACAGGGAACCCACAATAATATTTTCGGAACACCCTG‑GGTGTA‑CAATTCCCCGCGCCTGAAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAAt                                                                                                                                                                                                        <  1:78922/240‑1 (MQ=255)                                          cccccgggaaaaGAAATACAAGCACCGGCTAATGCCAGCATTTTGGTTTTT‑CGCTCCGAAATGGAATCCACAATGATAATTTCGGTACACCCTG‑GGTGTACCATTC‑CCCGCGCCTGTAAAAATTCAGGGACCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTg                                                                                                                                                                                                      <  1:336269/242‑1 (MQ=255)                                                    aaGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCa                                                                                                                                                                                              >  1:86210/1‑249 (MQ=255)                                                       aaaaaCAACCATCGGCAAATGCCGGCTTTTTGGCTTTT‑CGCTCCGACAGGAAACCCAAAATGATATTTTCGGTACACCCTG‑GGTGTACCATTC‑CCCGCGCCTGAAAAAATTCAGGGATCCCGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTgg                                                                                                                                                                                         <  1:651774/247‑1 (MQ=255)                                                         aTACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCg                                                                                                                                                                                       >  1:599655/1‑251 (MQ=255)                                                               ccATCGGCTAATGCCACCTTTTTGGTTTTT‑CGCTCAGACAGGAAACCCAAAATGATAATTTCGGTACACCCTT‑GTTGTCCCATTC‑CCCGCGCCTGAAAAAATTCGGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCt                                                                                                                                                                                 <  1:641563/250‑1 (MQ=255)                                                                    ggCTAATGCCAGCATTTTGGCTTTT‑CGCCCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTGACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCa                                                                                                                                                                            >  1:378705/1‑251 (MQ=255)                                                                                                 cccGACATGGAATCCACAATGATAATTTCGGTCCACCCTT‑GGTGTCCCATTT‑CCCGCGCCTGAAAAAATTCAGGGATCACGCCGATACCGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAAc                                                                                                                                                <  1:501041/250‑1 (MQ=255)                                                                                                  ccgacaggaaccccaaaaTGATATTTCCGGTACACCCTG‑GGTGT‑CCAATTCCCCGCGCCTGAAAAATTTCAGGGACCACGCCGATACCGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAAcc                                                                                                                                               <  1:377223/235‑1 (MQ=255)                                                                                                    ggacaggaaaccaaaaatgaaaTTTCCGTTCAACCTTGGGTGT‑CCAATTCCCCGCGCCTGAAAAAATTCGGGGATCCCGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAAcc                                                                                                                                               <  1:503583/230‑1 (MQ=255)                                                                                                    gACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACca                                                                                                                                              >  1:82711/1‑250 (MQ=255)                                                                                                     aCATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACcaca                                                                                                                                            >  1:63200/1‑251 (MQ=255)                                                                                                            aTCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCa                                                                                                                                     >  1:808322/1‑251 (MQ=255)                                                                                                              ccACAATAAAAATTTCGGTACACCCTT‑GGTGTCCCATTC‑CCCGCGCCTGAAAAAATTCGGGGATCACGCCAATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATcc                                                                                                                                                                                                                   <  1:251484/171‑1 (MQ=255)                                                                                                                 caATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGa                                                                                                                                >  1:224216/1‑251 (MQ=255)                                                                                                                 caATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGa                                                                                                                                >  1:709881/1‑251 (MQ=255)                                                                                                                    tGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTg                                                                                                                                                                                                      >  1:157992/1‑178 (MQ=255)                                                                                                                    tGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTg                                                                                                                             >  1:109857/1‑251 (MQ=255)                                                                                                                             cGGTACAACCTT‑GTTGTCCCAATC‑CCCGCCCCTGAAAAAATTCAGGGATCACGCCGATACCGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTa                                                                                                                    <  1:778811/251‑1 (MQ=255)                                                                                                                                ggtacaccttgggtgtcccattccccGCGCCTGAAAAATTTCGGGGACCCCGCCGATACCGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTGACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTa                                                                                                                    <  1:381256/227‑1 (MQ=255)                                                                                                                                aaaaaCCTG‑GGTG‑ACCAATTCCCCGCCCCTGTAAAAATCCGGGAACCACGCCAAAAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTAttt                                                                                                                 <  1:818925/248‑1 (MQ=255)                                                                                                                                    cccTT‑GGTGTCCCATTC‑CCCGCGCCTGAAAAATTTCAGGGATCCCGCCAATACCGGTCCCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTAt                                                                                                                                                                                                                                     <  1:390531/130‑1 (MQ=255)                                                                                                                                    aCCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAAt                                                                                                                                                                                                   >  1:521959/1‑165 (MQ=255)                                                                                                                                    aCCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGTGATAGCTCGGCAAATTGt                                                                                                                                                                      >  1:255015/1‑194 (MQ=255)                                                                                                                                      cttgggTGTACCATTC‑CCCGCCCCTGAAAAAATTCAGGAACCACGCCGATAACGTTCCCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCAt                                                                                                            <  1:237460/247‑1 (MQ=255)                                                                                                                                           gtgtACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTgg                                                                                                                                                                                         >  1:373830/1‑169 (MQ=255)                                                                                                                                           gtgtACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATa                                                                                                       >  1:361389/1‑251 (MQ=255)                                                                                                                                               aCCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGt                                                                                                                                                                                                                                   >  1:266476/1‑123 (MQ=255)                                                                                                                                                           cgcCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAAt                                                                                        >  1:699206/1‑251 (MQ=255)                                                                                                                                                                gaaaaaaTTCGGGGACCCCGCCGAAAACGGTCCCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAAttt                                                                                                  <  1:243481/234‑1 (MQ=255)                                                                                                                                                                aaaaaatttcggggccccggcggATAACGGTCCCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCat                                                                                   <  1:284782/229‑1 (MQ=255)                                                                                                                                                                 aaaaaaTTCGGGGACCACGCCGATACCGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCat                                                                                   <  1:303632/249‑1 (MQ=255)                                                                                                                                                                            ggacccccccaaTAACGTTCCCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATaa                                                                          <  1:719183/237‑1 (MQ=255)                                                                                                                                                                            ggATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATaa                                                                                                                                                  >  1:237128/1‑176 (MQ=255)                                                                                                                                                                                  cGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTc                                                                                                                                      >  1:159467/1‑182 (MQ=255)                                                                                                                                                                                      gATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATcc                                                                                                                                                                                                                   <  1:23267/101‑1 (MQ=255)                                                                                                                                                                                             gTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaa                                                       >  1:470545/1‑250 (MQ=255)                                                                                                                                                                                             gTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaa                                                       >  1:420898/1‑250 (MQ=255)                                                                                                                                                                                              tACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaac                                                      <  1:485789/250‑1 (MQ=255)                                                                                                                                                                                               cccgccgTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaacaa                                                    <  1:760868/250‑1 (MQ=255)                                                                                                                                                                                                   ccgTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaaca                                                     >  1:574863/1‑246 (MQ=255)                                                                                                                                                                                                        ggAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAg                                           >  1:633271/1‑251 (MQ=255)                                                                                                                                                                                                         gAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAg                                           <  1:122155/250‑1 (MQ=255)                                                                                                                                                                                                               aCCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATaacaa                                                    <  1:774095/235‑1 (MQ=255)                                                                                                                                                                                                                    aTCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGt                                                           >  1:183879/1‑223 (MQ=255)                                                                                                                                                                                                                     tCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTg                               >  1:471507/1‑250 (MQ=255)                                                                                                                                                                                                                        gCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTgcag                            >  1:830367/1‑250 (MQ=255)                                                                                                                                                                                                                              acGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGc                                          <  1:610448/230‑1 (MQ=255)                                                                                                                                                                                                                                 cccATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAatttatt                   <  1:89645/250‑1 (MQ=255)                                                                                                                                                                                                                                                tttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa   <  1:811501/251‑1 (MQ=255)                                                                                                                                                                                                                                                tttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa   <  1:347738/251‑1 (MQ=255)                                                                                                                                                                                                                                                tttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa   <  1:162387/251‑1 (MQ=255)                                                                                                                                                                                                                                                tttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGaa   <  1:440542/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ttCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTTCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAAt  <  1:179978/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGACAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTT‑CGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTT‑GGTGTACCAATT‑CCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCACCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCCTAATTGAATAGTCACTTGGCGATAGCTCGGCAAATTGTCGAGTGCCGCGCCACAATAAACCACAATGTTCATTTGATTGCCTCAGTTATTTTGCATTGATAAATTTTACGCCGAATTCCATATGGAATAAATCGCAACAATAGGTATAACAACCTCCAAAGCATGATATTTTGCAGCAATTTATTTTTGCTAATGCTAGAAT  >  1/2047811‑2048298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑