Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,043,053	T→C	Y27C (TAC→TGC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,043,053	0	T	C	100.0%	199.9 / NA	66	Y27C (TAC→TGC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/27);  total (39/27)

ACCGGGCATGGGCAAACTTTTCGTTAACGGCGCGCTTAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGTAACTGGAAGGGCGCAGCCTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTGGTGCGTACCGCCAT  >  1/2042806‑2043294                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  aCCGGGCATGGGCAAACTTTTCGTTAACGGCGCGCTTAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAAc                                                                                                                                                                                                                                                <  1:393217/251‑1 (MQ=255)       cATGGGCAAACTTTTCGTTAACGGCGCGCTTAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAg                                                                                                                                                                                                                                          <  1:857111/251‑1 (MQ=255)                                gcgcTTAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGgcgc                                                                                                                                                                                                                                     >  1:76073/1‑231 (MQ=255)                                   cTTAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAAc                                                                                                                                                                                                               <  1:134955/250‑1 (MQ=255)                                    ttAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACgg                                                                                                                                                                                                             <  1:899860/251‑1 (MQ=255)                                             attattCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTttcttc                                                                                                                                                                                                    <  1:290570/251‑1 (MQ=255)                                              ttattCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTttcttc                                                                                                                                                                                                    >  1:734662/1‑250 (MQ=255)                                                   cccTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAAt                                                                                                                                                                                              >  1:126471/1‑251 (MQ=255)                                                     cTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAGCCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATaa                                                                                                                                                                                            >  1:509846/1‑251 (MQ=255)                                                        gTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAggg                                                                                                                                                                                                                                        >  1:258678/1‑204 (MQ=255)                                                        gTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAg                                                                                                                                                                                          >  1:719654/1‑250 (MQ=255)                                                        gTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAg                                                                                                                                                                                          >  1:261834/1‑250 (MQ=255)                                                                       aTTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGttt                                                                                                                                                                          <  1:888914/251‑1 (MQ=255)                                                                             gTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCa                                                                                                                                                                     <  1:680074/250‑1 (MQ=255)                                                                                 gCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCACCTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGGCAGTTTAATCATGTTg                                                                                                                                                                >  1:433515/1‑251 (MQ=255)                                                                                        gACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCa                                                                                                                                                                     <  1:186655/239‑1 (MQ=255)                                                                                          cTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGGTAt                                                                                                                                                        >  1:710093/1‑250 (MQ=255)                                                                                          cTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGGGGGCAGTTTAATCATGTTGGGTGTTAt                                                                                                                                                        >  1:286272/1‑250 (MQ=255)                                                                                                             aGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCATTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTg                                                                                                                                    >  1:572337/1‑251 (MQ=255)                                                                                                                ggATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTgcg                                                                                                                                  >  1:19431/1‑250 (MQ=255)                                                                                                                 gATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTgcgc                                                                                                                                 <  1:215084/250‑1 (MQ=255)                                                                                                                    atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGaa                                                                                                                                                                                                                                           >  1:48088/1‑141 (MQ=255)                                                                                                                    atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTg                                                                                                                                                    >  1:106905/1‑228 (MQ=255)                                                                                                                    atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGc                                                                                                                                                   >  1:282196/1‑229 (MQ=255)                                                                                                                    atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTgcgc                                                                                                                                 >  1:279985/1‑247 (MQ=255)                                                                                                                    atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGccc                                                                                                                               >  1:230760/1‑249 (MQ=255)                                                                                                                    atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGccc                                                                                                                               >  1:129964/1‑249 (MQ=255)                                                                                                                      tttATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGGGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCg                                                                                                                           >  1:593138/1‑251 (MQ=255)                                                                                                                            cGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGGCAGTTTAATCATGt                                                                                                                                                                  >  1:190950/1‑206 (MQ=255)                                                                                                                              ccGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACgg                                                                                                                                                                                                             >  1:874507/1‑161 (MQ=255)                                                                                                                              ccGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTccc                                                                                                                    <  1:457173/250‑1 (MQ=255)                                                                                                                              ccGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTcc                                                                                                                     >  1:356492/1‑249 (MQ=255)                                                                                                                                  gattgatCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTac                                                                                                                                                        <  1:884953/210‑2 (MQ=255)                                                                                                                                  gattgatCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTAt                                                                                                                                                        <  1:884970/210‑1 (MQ=255)                                                                                                                                   attgatCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATTGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTAc                                                                                                              <  1:786582/251‑1 (MQ=255)                                                                                                                                   attgatCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTAc                                                                                                              <  1:741241/251‑1 (MQ=255)                                                                                                                                               aTTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGacac                                                                                                  <  1:597461/251‑1 (MQ=255)                                                                                                                                                    ttattaAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCg                                                                                                                                                                                                           <  1:288169/141‑1 (MQ=255)                                                                                                                                                    ttattaAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAg                                                                                                      <  1:807488/242‑1 (MQ=255)                                                                                                                                                      attaAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGttt                                                                                                                                                                          <  1:19761/172‑1 (MQ=255)                                                                                                                                                            gAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatg                                                                                     >  1:225320/1‑251 (MQ=255)                                                                                                                                                             aaGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatga                                                                                    <  1:567810/251‑1 (MQ=255)                                                                                                                                                                tAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatgatg                                                                                  <  1:130435/250‑1 (MQ=255)                                                                                                                                                                tAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatgat                                                                                   <  1:442575/249‑1 (MQ=255)                                                                                                                                                                tAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGG‑AGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatgatg                                                                                  <  1:101641/249‑1 (MQ=255)                                                                                                                                                                          aaaaaTGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGac                                                                                                    >  1:768229/1‑222 (MQ=255)                                                                                                                                                                              atgatgGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGa                                                                                                                                                                                                                       >  1:252934/1‑103 (MQ=255)                                                                                                                                                                                   ggTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatgat                                                                                   >  1:717408/1‑230 (MQ=255)                                                                                                                                                                                   ggTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGa                                                              >  1:50361/1‑251 (MQ=255)                                                                                                                                                                                    gtgtAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAg                                                                                                      >  1:692240/1‑210 (MQ=255)                                                                                                                                                                                    gtgtAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACat                                                                                      >  1:438325/1‑226 (MQ=255)                                                                                                                                                                                            ccAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACat                                                                                      >  1:352769/1‑218 (MQ=255)                                                                                                                                                                                            ccAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCa                                                     <  1:629308/251‑1 (MQ=255)                                                                                                                                                                                              aaTAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCAtt                                                   <  1:174667/251‑1 (MQ=255)                                                                                                                                                                                              aaTAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCAtt                                                   <  1:828564/251‑1 (MQ=255)                                                                                                                                                                                                       aaaaaaTACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATc                                                                                                                                                                      >  1:891212/1‑127 (MQ=255)                                                                                                                                                                                                        aaaaaTGCCGAAATTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTAc                                         <  1:752613/251‑1 (MQ=255)                                                                                                                                                                                                            aTACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGac                                                                                                    >  1:406581/1‑188 (MQ=255)                                                                                                                                                                                                                     ttgttgAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCgg                             >  1:783825/1‑250 (MQ=255)                                                                                                                                                                                                                              aacTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGaa                                                             >  1:187920/1‑209 (MQ=255)                                                                                                                                                                                                                                cTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAAc                                                                                        >  1:335936/1‑180 (MQ=255)                                                                                                                                                                                                                                cTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCtttt                 >  1:240695/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTgg               <  1:580704/245‑1 (MQ=255)                                                                                                                                                                                                                                               gATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTGGTGCGTACCGCCAt  >  1:262134/1‑251 (MQ=255)                                                                                                                                                                                                                                               gATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTGGTGCGTACCGCCAt  >  1:325899/1‑251 (MQ=255)                                                                                                                                                                                                                                               gATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGCCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCAt                                                                                                                                            >  1:275276/1‑113 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ACCGGGCATGGGCAAACTTTTCGTTAACGGCGCGCTTAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGTAACTGGAAGGGCGCAGCCTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTGGTGCGTACCGCCAT  >  1/2042806‑2043294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑