Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,035,067	T→C	I87V (ATC→GTC)	unknown ←	Octaprenyl diphosphate synthase (EC 2.5.1.90)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,035,067	0	T	C	100.0%	195.7 / NA	67	I87V (ATC→GTC)	unknown	Octaprenyl diphosphate synthase (EC 2.5.1.90)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/34);  total (33/34)

TGGTTTGCGGATCATTTACGTTCATTAATTGTTGCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGATGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTACTTTTTGCATATCCCCATGGCTTAACGCCTGGATTG  >  1/2034851‑2035312                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      tGGTTTGCGGATCATTTACGTTCATTAATTGTTGCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATa                                                                                                                                                                                                                     <  1:608212/251‑1 (MQ=255)     ttGCGGATCATTTACGTTCATTAATTGTTGCCCTTCTCCTTCCGCCAACACATAAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTAGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACt                                                                                                                                                                                                                 <  1:333515/251‑1 (MQ=255)          gATCATTTACGTTCATTAATTGTTGCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATaaa                                                                                                                                                                                                                                    >  1:737412/1‑227 (MQ=255)            tCATTTACGTTCATTAATTGTTGCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACaaa                                                                                                                                                                                                          >  1:611687/1‑251 (MQ=255)                   cGTTCATTAATTGTTGCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGca                                                                                                                                                                                                   >  1:836153/1‑251 (MQ=255)                             ttgttgCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGt                                                                                                                                                                                              >  1:24951/1‑246 (MQ=255)                              ttttgCACTTCTCCTTCCGCCACCACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGc                                                                                                                                                                                        <  1:582477/249‑1 (MQ=255)                              tgttgCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGc                                                                                                                                                                                        <  1:625481/251‑1 (MQ=255)                              tgttgCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGc                                                                                                                                                                                        <  1:38174/251‑1 (MQ=255)                              tgttgCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGc                                                                                                                                                                                        <  1:254916/251‑1 (MQ=255)                               tttgCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCt                                                                                                                                                                                       <  1:377186/250‑1 (MQ=255)                                     cTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTtgctg                                                                                                                                                                                  >  1:454951/1‑250 (MQ=255)                                                       caTTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGtaataa                                                                                                                                                                                                                                     >  1:391863/1‑181 (MQ=255)                                                       caTTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACaa                                                                                                                                                                                                           >  1:13078/1‑207 (MQ=255)                                                       caTTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCa                                                                                                                                                               >  1:633976/1‑251 (MQ=255)                                                       caTTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGAT‑GCTTGCTGCCCATCGAAACCCAATGCa                                                                                                                                                               >  1:187745/1‑250 (MQ=255)                                                         ttAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACg                                                                                                                                                             <  1:474899/251‑1 (MQ=255)                                                                   ccGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCaa                                                                                                                                                    >  1:383772/1‑250 (MQ=255)                                                                     gCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACTGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATa                                                                                                                                                                                                                     <  1:273110/183‑1 (MQ=255)                                                                     gCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCgtgt                                                                                                                                                                                                                                          <  1:63103/162‑1 (MQ=255)                                                                     gCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATa                                                                                                                                                                                                                     >  1:835862/1‑183 (MQ=255)                                                                      ccATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAacac                                                                                                                                                 <  1:630827/250‑1 (MQ=255)                                                                      ccATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAAcaca                                                                                                                                                >  1:388217/1‑251 (MQ=255)                                                                      ccATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAAcaca                                                                                                                                                >  1:72262/1‑251 (MQ=255)                                                                                tAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCg                                                                                                                                      <  1:17231/251‑1 (MQ=255)                                                                                             gACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATa                                                                                                                                                                                                                     >  1:836786/1‑159 (MQ=255)                                                                                             gACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCtt                                                                                                                         >  1:467100/1‑251 (MQ=255)                                                                                                     cTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCAc                                                                                                                 <  1:74078/251‑1 (MQ=255)                                                                                                            aCCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGcac                                                                                                          <  1:697247/251‑1 (MQ=255)                                                                                                                aTTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAgg                                                                                                                                                                                               <  1:835548/162‑1 (MQ=255)                                                                                                                      aCGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGaaa                                                                                                <  1:751668/251‑1 (MQ=255)                                                                                                                       cgcgcgGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAAc                                                                                               <  1:600322/251‑1 (MQ=255)                                                                                                                         cgcgGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAAccc                                                                                             <  1:801064/251‑1 (MQ=255)                                                                                                                         cgcgGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAAcc                                                                                              <  1:500233/250‑1 (MQ=255)                                                                                                                             ggTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATa                                                                                                                                                                                                                     >  1:266836/1‑127 (MQ=255)                                                                                                                             ggTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGaa                                                                                                                                >  1:575940/1‑212 (MQ=255)                                                                                                                             ggTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGcac                                                                                                          >  1:746283/1‑234 (MQ=255)                                                                                                                             ggTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGcac                                                                                                          >  1:207641/1‑234 (MQ=255)                                                                                                                             ggTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGt                                                                                                     <  1:240403/239‑1 (MQ=255)                                                                                                                             ggTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCaa                                                                                           >  1:301522/1‑249 (MQ=255)                                                                                                                              gTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGACACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGcac                                                                                                          >  1:429855/1‑233 (MQ=255)                                                                                                                                      aaaaTCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAATCCCAACTGattaatta                                                                                <  1:202604/250‑1 (MQ=255)                                                                                                                                       aaaTCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAAc                                                                                               >  1:653089/1‑235 (MQ=255)                                                                                                                                       aaaTCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGattaatta                                                                                <  1:336626/250‑1 (MQ=255)                                                                                                                                             cccaccAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCcac                                                                         <  1:125131/251‑1 (MQ=255)                                                                                                                                               caccaACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACAt                                                                       >  1:211595/1‑251 (MQ=255)                                                                                                                                                accaACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATc                                                                      <  1:667377/251‑1 (MQ=255)                                                                                                                                                 ccaACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGcac                                                                                                                                                                                                  >  1:881922/1‑126 (MQ=255)                                                                                                                                                 ccaACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGaaa                                                                                                >  1:730332/1‑224 (MQ=255)                                                                                                                                                     cacGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACt                                                                                                                                                                                                                 >  1:720864/1‑107 (MQ=255)                                                                                                                                                                    aCCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCt                                                                                                                          <  1:794589/179‑1 (MQ=255)                                                                                                                                                                     ccGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAAt                                                 >  1:529469/1‑251 (MQ=255)                                                                                                                                                                     ccGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAAt                                                 >  1:822500/1‑251 (MQ=255)                                                                                                                                                                     ccGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAAt                                                 <  1:1392/251‑1 (MQ=255)                                                                                                                                                                                 ttCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTAc                                     <  1:504828/251‑1 (MQ=255)                                                                                                                                                                                   cGCCGTGGCACGCCCGCGACGCATATCCGATTCATCAACGACATAGTCTTGTAATAAAGACGCGGTATGAATAAACTCGAAAAAAGTACAACAGGTGATGAATTGATGAACACAGAAACCAAATGAACGAGAAGAAAAAAAAGAGATAAGCGGAAGAATACGCTTAAAGCCAACTTGCACAATGTAGAAAAACAACTGATTAATTAAAGAAACATCGGAGTTAAGTTGGGCTAAAATTGATTAGTTTaatt                                   <  1:308424/251‑4 (MQ=255)                                                                                                                                                                                          gCACGCCCGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTACTTTTTGCat                            >  1:264528/1‑251 (MQ=255)                                                                                                                                                                                                ccGCGACGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTACTTTTTGCATATcccc                      <  1:60649/251‑1 (MQ=255)                                                                                                                                                                                                     aCGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGc                                                                                                                                                                                        >  1:468346/1‑84 (MQ=255)                                                                                                                                                                                                     aCGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAGTGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTACTTTTTGCATATCCCCATGGc                 <  1:539874/251‑1 (MQ=255)                                                                                                                                                                                                     aCGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGcac                                                                                                          >  1:820072/1‑162 (MQ=255)                                                                                                                                                                                                     aCGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGt                                                            >  1:821912/1‑208 (MQ=255)                                                                                                                                                                                                     aCGCATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTACTTTTTGCATATCCCCATGGc                 <  1:523254/251‑1 (MQ=255)                                                                                                                                                                                                        cATATCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTACTTTTTGCATATCCCCATGGCtt               <  1:23491/250‑1 (MQ=255)                                                                                                                                                                                                           atCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAAccc                                                                                             <  1:72935/169‑1 (MQ=255)                                                                                                                                                                                                           atCCGATTCATCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTACTTTTTGCATATCCCCATGGCTTAACg           <  1:139763/251‑1 (MQ=255)                                                                                                                                                                                                                    aTCGACGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTACTTTTTGCATATCCCCATGGCTTAACGCCTGGATTg  >  1:551257/1‑251 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TGGTTTGCGGATCATTTACGTTCATTAATTGTTGCACTTCTCCTTCCGCCAACACATTAGTGGCATCCGCCATAATGCGTAAAATTTTCAAAGACTCCAGCTGCGCAACCAATTGGAACGCGCGGGTATAAATGAAATCACCCACCAACACGCTTGCCGCATTACCGAATTCCGCATTCGCCGTGGCACGCCCGCGACGCATATCCGATTCATCGATGACATCGTCGTGTAATAAAGACGCGGTATGAATAAACTCGACAAAAGTCGCACAGGTGATGGCTTGCTGCCCATCGAAACCCAATGCACGAGCCGCCAACACAGCGATAAGCGGACGAATACGCTTGCCGCCACCTTGCACAATGTAGAAACCCAACTGATTAATTAACGCCACATCGGAGTTCAGTTGGGCTAAAATTGATTCGTTTACTTTTTGCATATCCCCATGGCTTAACGCCTGGATTG  >  1/2034851‑2035312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑