Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,000,256	T→C	Q232R (CAA→CGA)	unknown ←	Exodeoxyribonuclease V gamma chain (EC 3.1.11.5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,000,256	0	T	C	100.0%	175.1 / NA	61	Q232R (CAA→CGA)	unknown	Exodeoxyribonuclease V gamma chain (EC 3.1.11.5)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/31);  total (30/31)

GTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTTGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGATTTGTCGGCGAATGTCGTCGTCTTGCTGATTTTCCCAAGCGGCAATCCAG  >  1/2000014‑2000486                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        gtgCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCa                                                                                                                                                                                                                                >  1:180491/1‑251 (MQ=255) gtgCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCa                                                                                                                                                                                                                                >  1:534361/1‑251 (MQ=255)       ccTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACgg                                                                                                                                                                                                                             <  1:782057/248‑1 (MQ=255)          gTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGtttt                                                                                                                                                                                                                       >  1:674920/1‑251 (MQ=255)                    taataaAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCCCGGGGTTTTTCTAATAGCa                                                                                                                                                                                                             >  1:377645/1‑251 (MQ=255)                    taataaAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCa                                                                                                                                                                                                             >  1:253283/1‑251 (MQ=255)                     aataaAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAg                                                                                                                                                                                                            <  1:101477/251‑1 (MQ=255)                     aataaAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAg                                                                                                                                                                                                            >  1:83955/1‑251 (MQ=255)                           ggCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTg                                                                                                                                                                                                      >  1:700832/1‑251 (MQ=255)                               tATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACgg                                                                                                                                                                                                                             <  1:192500/224‑1 (MQ=255)                               tATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCaaa                                                                                                                                                                                                  >  1:795221/1‑251 (MQ=255)                                 tCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATg                                                                                                                                                                                                <  1:479295/251‑1 (MQ=255)                                 tCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATg                                                                                                                                                                                                <  1:692014/251‑1 (MQ=255)                                         aTTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCggg                                                                                                                                                                                        >  1:791608/1‑250 (MQ=255)                                            aaaaTAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGtgtt                                                                                                                                                                                     >  1:294189/1‑251 (MQ=255)                                                taatCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTAc                                                                                                                                                                                 <  1:280984/251‑1 (MQ=255)                                                  atCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCa                                                                                                                                                                               <  1:40914/251‑1 (MQ=255)                                                      gTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAAt                                                                                                                                                                            <  1:248833/250‑1 (MQ=255)                                                         cGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTAc                                                                                                                                                                                 >  1:596641/1‑242 (MQ=255)                                                                 cAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGATTTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTa                                                                                                                                                                 <  1:364675/251‑1 (MQ=255)                                                                   aCGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATc                                                                                                                                                               >  1:31384/1‑250 (MQ=255)                                                                           ttttCTACGAATTCCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCAA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCAc                                                                                                                                                      <  1:558073/251‑1 (MQ=255)                                                                                         cGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAgg                                                                                                                                        >  1:302332/1‑251 (MQ=255)                                                                                         cGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAgg                                                                                                                                        <  1:258157/251‑1 (MQ=255)                                                                                          ggATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAgg                                                                                                                                        >  1:251665/1‑250 (MQ=255)                                                                                           gATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGgc                                                                                                                                       <  1:272240/250‑1 (MQ=255)                                                                                           gATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGgc                                                                                                                                       <  1:567099/250‑1 (MQ=255)                                                                                                      tCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAAt                                                                                                                           <  1:702664/251‑1 (MQ=255)                                                                                                         ccccAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGc                                                                                                                         <  1:850218/250‑1 (MQ=255)                                                                                                          cccAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTACCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGc                                                                                                                         >  1:240339/1‑249 (MQ=255)                                                                                                           ccAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCAc                                                                                                                                                      >  1:462128/1‑219 (MQ=255)                                                                                                           ccAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCtt                                                                                                                      >  1:587824/1‑251 (MQ=255)                                                                                                                  tCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATg                                                                                                                >  1:158026/1‑250 (MQ=255)                                                                                                                    cTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGa                                                                                                             <  1:430397/251‑1 (MQ=255)                                                                                                                     tCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGat                                                                                                            >  1:91586/1‑251 (MQ=255)                                                                                                                      cGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTg                                                                                                                                                                                                      >  1:379919/1‑160 (MQ=255)                                                                                                                                        aCAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGcc                                                                                         <  1:194781/251‑1 (MQ=255)                                                                                                                                         cAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGt                                                                                                   <  1:98376/240‑1 (MQ=255)                                                                                                                                                 caTCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTAc                                                                                                                                                                                 <  1:601846/154‑1 (MQ=255)                                                                                                                                                 caTCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCg                                                                                                    <  1:454734/231‑1 (MQ=255)                                                                                                                                                 caTCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGt                                                                                <  1:807021/251‑1 (MQ=255)                                                                                                                                                   tCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTAc                                                                                                                                                                                 <  1:34848/152‑1 (MQ=255)                                                                                                                                                   tCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCt                                                                                                                                                           <  1:503382/174‑1 (MQ=255)                                                                                                                                                   tCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGatat                                                                                                          <  1:333777/223‑1 (MQ=255)                                                                                                                                                   tCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATAttt                                                                                                        <  1:7086/225‑1 (MQ=255)                                                                                                                                                   tCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGtt                                                                               >  1:510316/1‑250 (MQ=255)                                                                                                                                                   tCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGtt                                                                               >  1:75774/1‑250 (MQ=255)                                                                                                                                                   tCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGtt                                                                               >  1:346549/1‑250 (MQ=255)                                                                                                                                                        atatTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGcc                                                                                                                        <  1:740655/204‑1 (MQ=255)                                                                                                                                                        atatTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCattat                                                                         >  1:355513/1‑251 (MQ=255)                                                                                                                                                             ggCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACg                                                                                 <  1:213477/238‑1 (MQ=255)                                                                                                                                                                               tttCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGAtt                                                  >  1:215053/1‑251 (MQ=255)                                                                                                                                                                                 tCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGATTTg                                                <  1:890999/251‑1 (MQ=255)                                                                                                                                                                                     aaTAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATg                                                                                                                                                                                                >  1:701865/1‑103 (MQ=255)                                                                                                                                                                                          gCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACt                                                                  >  1:693597/1‑224 (MQ=255)                                                                                                                                                                                          gCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGATTTGTCGGCGAAt                                       >  1:227909/1‑251 (MQ=255)                                                                                                                                                                                          gCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGATTTGTCGGCGAAt                                       >  1:409588/1‑251 (MQ=255)                                                                                                                                                                                                            aTGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGATTTGTCGGCGAATGTCGTCGTCTTGCTGAtt                     <  1:586233/251‑1 (MQ=255)                                                                                                                                                                                                                     aTAAACAGGCGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGATTTGTCGGCGAATGTCGTCGTCTTGCTGATTTTCCCAAg             >  1:379741/1‑250 (MQ=255)                                                                                                                                                                                                                              cGTTCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGATTTGTCGGCGAATGTCGTCGTCTTGCTGATTTTCCCAAGCGGCAATcc    <  1:271442/250‑1 (MQ=255)                                                                                                                                                                                                                                ttCCGGCAAAAAGAGCGGTCGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGATTTGTCGGCGAATGTCGTCGTCTTGCTGATTTTCCCAAGCGGCAATCCAg  <  1:396156/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        GTGCAGCCTGTTCCGCCGATAATAAAGGCTTATCTGCGTGATTAAAATAATCGGTGCGAGTACGCAACGCCAGTTTTTCTACGAATTTCGGATCAACGATGTCGCCCCAATATTCCTCGCAGGGATTATTAAAAAACAAGTGCACATCGCAATATTGGCTGATTGCCTGAAAAATTTCCAAATAAGCCTTTGGCAAGGCGGGAATGCCGAAAATAAACAGGCGTTCCGGCAAAAAGAGCGGTTGA‑TTTTCACGGAGTTTTTCTAATAGCAGTTGGTGCAAATGGGCGCGGTGTTGTACCAAATCCAGCCCGGTATCCGCTTTCACCGCCCGCACCAAGGCGCGCCACAAAATGCCTTGCCATGCGATATTTTGCGTAATCTGCGCCAGTAAACGTTCATTATTTAGACTTAATTGGCTCTCGATTTGTCGGCGAATGTCGTCGTCTTGCTGATTTTCCCAAGCGGCAATCCAG  >  1/2000014‑2000486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑