Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	102,543	T→C	A73A (GCA→GCG)	unknown ←	DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	102,543	0	T	C	100.0%	97.8 / NA	35	A73A (GCA→GCG)	unknown	DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/15);  total (20/15)

TTTTAAAAGTTAAATTGCTCATAAAAGATCCTTTTTTGTATCTTTTAAGTTCTTTTTCATTTATGATTAAAATTTATTCGTTAAAACAGGCTTGCGATTACGTTCAATCCGCTCAAACGCCCGCGCTGCCCAAATCTGTTCAGGGGGAATGCCGACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTGGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGTAATATTTGATACACTCATTAACTACATTCCTTTTTATGTGT  >  1/102417‑102784                                                                                                                               |                                                                                                                                                                                                                                                  cttttatacttaaaccacTCATAAAAGATCC‑TTTTTGTATCTTTTAATTTTATTTTCATTTATGATTAAAACTTATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGt                                                                                                                                                                                 >  1:738232/18‑192 (MQ=255) cttttatacttaaaccacTCATAAAAGATCC‑TTTTTGTATCTTTTAATTTTATTTTCATTTATGATTAAAACTTATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAAc                                                                                                                                                  <  1:726093/206‑1 (MQ=255) cttttatacttaaaccacTCATAAAAGATCC‑TTTTTGTATCTTTTAATTTTATTTTCATTTATGATTAAAACTTATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACg                                                                                                                      >  1:35402/18‑251 (MQ=255)        acttaaaccacTCATAAAAGATCC‑TTTTTGTATCTTTTAATTTTATTTTCATTTATGATTAAAACTTATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGa                                                                                                                >  1:165249/11‑250 (MQ=255)                                 tttttGTATCTTTTAATTTTATTTTCATTTATGATTAAAACTTATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGAc                                                                                                                                              >  1:345044/1‑196 (MQ=255)                                          cttttaattttattttCATTTATGATTAAAACTTATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATc                                                                               >  1:788942/13‑250 (MQ=255)                                                          cATTTATGATTAAAACTTATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCAt                                                              >  1:253653/1‑251 (MQ=255)                                                                 gATTAAAACTTATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGc                                                                                                                                                                      >  1:723544/1‑140 (MQ=255)                                                                 gATTAAAACTTATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCtgtg                                                        >  1:311654/1‑250 (MQ=255)                                                                       aacttATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGtttt                                                 >  1:196317/4‑251 (MQ=255)                                                                          ttATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGttttttt                                              >  1:1300/1‑251 (MQ=255)                                                                          ttATTGGTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGttttttt                                              >  1:499667/1‑251 (MQ=255)                                                                               ggTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTaaaa                                                                                   <  1:43834/208‑1 (MQ=255)                                                                               ggTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGc                                                                     >  1:581099/2‑223 (MQ=255)                                                                               ggTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAgaa                                          >  1:291068/2‑248 (MQ=255)                                                                               ggTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAgaa                                          >  1:420659/2‑248 (MQ=255)                                                                               ggTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAgaa                                          >  1:895318/2‑248 (MQ=255)                                                                               ggTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAgaa                                          >  1:649784/2‑248 (MQ=255)                                                                                gTTAGAACAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAgaac                                         <  1:584009/250‑4 (MQ=255)                                                                                    gaaCAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAg                                                                                                                                                                                                                    >  1:523130/2‑75 (MQ=255)                                                                                    gaaCAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAAc                                                                                                                                                  <  1:340546/140‑1 (MQ=255)                                                                                    gaaCAGGCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAgaac                                         >  1:319796/2‑243 (MQ=255)                                                                                         ggCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAgaac                                         >  1:762124/1‑238 (MQ=255)                                                                                          gCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTAtttaa                                  <  1:97835/247‑3 (MQ=255)                                                                                          gCTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATac                               <  1:820011/250‑1 (MQ=255)                                                                                           cTTGCGATTACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATacac                             <  1:268667/251‑1 (MQ=255)                                                                                                   tACGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAAc                     <  1:838453/251‑1 (MQ=255)                                                                                                    aCGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAAc                     <  1:354921/250‑1 (MQ=255)                                                                                                     cGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTAttt                                    <  1:851335/234‑1 (MQ=255)                                                                                                     cGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTa                   <  1:659063/251‑1 (MQ=255)                                                                                                     cGTTCAATCCGCTCAAGCGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTa                   <  1:296991/251‑1 (MQ=255)                                                                                                                   aagcGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTAtg     <  1:232557/248‑1 (MQ=255)                                                                                                                   aagcGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTAtg     <  1:459791/248‑1 (MQ=255)                                                                                                                   aagcGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTAtg     <  1:101033/248‑1 (MQ=255)                                                                                                                      cGCCCGAGCCGCCCAAATTTGTTCTGGGGCAACGCCAACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTCGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGAACTATTTAATACACTCATTAACTACATTCCTTTTTATgtgt  >  1:329533/1‑251 (MQ=255)                                                                                                                               |                                                                                                                                                                                                                                                  TTTTAAAAGTTAAATTGCTCATAAAAGATCCTTTTTTGTATCTTTTAAGTTCTTTTTCATTTATGATTAAAATTTATTCGTTAAAACAGGCTTGCGATTACGTTCAATCCGCTCAAACGCCCGCGCTGCCCAAATCTGTTCAGGGGGAATGCCGACAGCATTAGCAATCAATCTTTCCATTTTCGGATAAGGTTTATCTAAAGCTGATTTCAATGTGTTGTAACTGACATTGCCGGCTTTGGCTAAAGAACGTAACGACCATCCGTTTTTTCGTAACGCCGCTAAAATATCCGCTCGATGCCAATCATGCTGTGCGGTTTTTTTAGTAATATTTGATACACTCATTAACTACATTCCTTTTTATGTGT  >  1/102417‑102784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑