Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	102,256	T→G	E61D (GAA→GAC)	unknown ←	DNA transposition protein Mup03, A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	102,256	0	T	G	100.0%	85.8 / NA	31	E61D (GAA→GAC)	unknown	DNA transposition protein Mup03, A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/13);  total (18/13)

CCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATCTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATGGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCGGCCAAAACAGGGAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCAGCTTGCACTTCTTCCGGCATGGATTTGACGGCGTATTCGTAACCGCCTCCCAAACCGGAGCGT  >  1/102011‑102310                                                                                                                                                                                                                                                      |                                                       tcATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACgtcttc                                                   <  1:176498/250‑1 (MQ=255)       tGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCAt                                             <  1:403288/251‑1 (MQ=255)            gtgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtt                                        >  1:192493/1‑251 (MQ=255)             tgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttt                                       >  1:132299/1‑251 (MQ=255)              gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:219693/1‑250 (MQ=255)              gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:275209/1‑250 (MQ=255)              gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:349524/1‑250 (MQ=255)              gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:615288/1‑250 (MQ=255)                ttATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCTCCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAGCAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtttaac                                    <  1:726568/251‑4 (MQ=255)                 tATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   <  1:718854/251‑1 (MQ=255)                 tATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   <  1:656371/251‑1 (MQ=255)                   tGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  >  1:759226/1‑249 (MQ=255)                   tGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  >  1:329996/1‑249 (MQ=255)                   tGGCATACCAATGCCAACACATCCAAAATCTTCATGCCTCCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  <  1:493914/250‑2 (MQ=255)                        tACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTTATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  <  1:453111/245‑2 (MQ=255)                         aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtttaacaccatatt                            >  1:98014/1‑239 (MQ=255)                         aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgccatatt                            >  1:70975/1‑243 (MQ=255)                         aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgccatatt                            >  1:747238/1‑243 (MQ=255)                            aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:203677/251‑1 (MQ=255)                            aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:128577/251‑1 (MQ=255)                            aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGGTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:125781/251‑1 (MQ=255)                              tGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAAc                      <  1:614694/251‑1 (MQ=255)                                      cATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCCCCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCCCCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccacca              <  1:404859/251‑5 (MQ=255)                                      cATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccacca              >  1:415674/1‑247 (MQ=255)                                        tCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   >  1:615411/1‑228 (MQ=255)                                         ccAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgc           <  1:714588/251‑8 (MQ=255)                                          cAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgc           >  1:725995/1‑243 (MQ=255)                                                  tCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgcccaaaacgt  >  1:527270/1‑235 (MQ=255)                                                                                                                       gCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAAc                      >  1:624742/1‑162 (MQ=255)                                                                                                                                      gTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCgg                                                <  1:73909/121‑1 (MQ=255)                                                                                                                                      gTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCg                          >  1:341248/1‑143 (MQ=255)                                                                                                                                                 gCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        >  1:197573/1‑134 (MQ=255)                                                                                                                                                                                                                                                      |                                                       CCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATCTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATGGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCGGCCAAAACAGGGAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCAGCTTGCACTTCTTCCGGCATGGATTTGACGGCGTATTCGTAACCGCCTCCCAAACCGGAGCGT  >  1/102011‑102310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑