Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	102,247	A→G	A64A (GCT→GCC)	unknown ←	DNA transposition protein Mup03, A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	102,247	0	A	G	100.0%	93.5 / NA	34	A64A (GCT→GCC)	unknown	DNA transposition protein Mup03, A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

CCGGTTGCAGGCCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATCTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATGGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCGGCCAAAACAGGGAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCAGCTTGCACTTCTTCCGGCATGGATTTGACGGCGTATTCGTAACCGCCTCCCAAACCGGAGCGT  >  1/102000‑102310                                                                                                                                                                                                                                                        |                                                                ccGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCt                                                              <  1:515353/251‑1 (MQ=255)      tGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCAc                                                         <  1:147129/251‑1 (MQ=255)            tcATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACgtcttc                                                   <  1:176498/250‑1 (MQ=255)                  tGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCAt                                             <  1:403288/251‑1 (MQ=255)                       gtgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtt                                        >  1:192493/1‑251 (MQ=255)                        tgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttt                                       >  1:132299/1‑251 (MQ=255)                         gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:219693/1‑250 (MQ=255)                         gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:275209/1‑250 (MQ=255)                         gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:615288/1‑250 (MQ=255)                         gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:349524/1‑250 (MQ=255)                           ttATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCTCCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAGCAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtttaac                                    <  1:726568/251‑4 (MQ=255)                            tATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   <  1:718854/251‑1 (MQ=255)                            tATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   <  1:656371/251‑1 (MQ=255)                              tGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  >  1:759226/1‑249 (MQ=255)                              tGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  >  1:329996/1‑249 (MQ=255)                              tGGCATACCAATGCCAACACATCCAAAATCTTCATGCCTCCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  <  1:493914/250‑2 (MQ=255)                                   tACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTTATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  <  1:453111/245‑2 (MQ=255)                                    aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtttaacaccatatt                            >  1:98014/1‑239 (MQ=255)                                    aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgccatatt                            >  1:70975/1‑243 (MQ=255)                                    aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgccatatt                            >  1:747238/1‑243 (MQ=255)                                       aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:203677/251‑1 (MQ=255)                                       aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:128577/251‑1 (MQ=255)                                       aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGGTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:125781/251‑1 (MQ=255)                                         tGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAAc                      <  1:614694/251‑1 (MQ=255)                                                 cATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCCCCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCCCCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccacca              <  1:404859/251‑5 (MQ=255)                                                 cATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccacca              >  1:415674/1‑247 (MQ=255)                                                   tCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   >  1:615411/1‑228 (MQ=255)                                                    ccAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgc           <  1:714588/251‑8 (MQ=255)                                                     cAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgc           >  1:725995/1‑243 (MQ=255)                                                             tCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgcccaaaacgt  >  1:527270/1‑235 (MQ=255)                                                                                                                                  gCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAAc                      >  1:624742/1‑162 (MQ=255)                                                                                                                                                 gTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCgg                                                <  1:73909/121‑1 (MQ=255)                                                                                                                                                 gTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCg                          >  1:341248/1‑143 (MQ=255)                                                                                                                                                            gCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        >  1:197573/1‑134 (MQ=255)                                                                                                                                                                                                                                                        |                                                                CCGGTTGCAGGCCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATCTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATGGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCGGCCAAAACAGGGAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCAGCTTGCACTTCTTCCGGCATGGATTTGACGGCGTATTCGTAACCGCCTCCCAAACCGGAGCGT  >  1/102000‑102310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑