Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	102,205	G→C	L78L (CTC→CTG)	unknown ←	DNA transposition protein Mup03, A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	102,205	0	G	C	100.0%	150.8 / NA	52	L78L (CTC→CTG)	unknown	DNA transposition protein Mup03, A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (31/21);  total (31/21)

CCACCAAGACTTAAGAGCACTCACTGTTACCGGTTTCTCGCCCGGTTGCAGGCCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATCTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATGGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCGGCCAAAACAGGGAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCAGCTTGCACTTCTTCCGGCATGGATTTGACGGCGTATTCGTAACCGCCTCCCAAACCGGAGCGT  >  1/101959‑102310                                                                                                                                                                                                                                                       |                                                                                                          ccaccaAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGAt                                                                                                       >  1:632240/1‑251 (MQ=255)  caccaAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGAtt                                                                                                      >  1:630106/1‑251 (MQ=255)       aGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCa                                                                                                 >  1:41983/1‑251 (MQ=255)        gACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCa                                                                                                 >  1:841580/1‑250 (MQ=255)        gACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGGCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCAAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCa                                                                                                 >  1:835758/1‑250 (MQ=255)         aCTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCAc                                                                                                <  1:619089/250‑1 (MQ=255)         aCTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCAc                                                                                                <  1:150007/250‑1 (MQ=255)            tAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCa                                                                                            >  1:70006/1‑251 (MQ=255)              agtgCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCa                                                                                            >  1:67207/4‑249 (MQ=255)               gtgCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACtt                                                                                         >  1:551902/3‑251 (MQ=255)                tgCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACttt                                                                                        <  1:135550/250‑1 (MQ=255)                    ctcaccGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGcc                                                                                    <  1:494922/251‑1 (MQ=255)                      caccgtgaCCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCaa                                                                                  >  1:98421/8‑251 (MQ=255)                        ccgtgaCCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGt                                                                                >  1:359769/6‑251 (MQ=255)                              ccGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTcac                                                                           >  1:215209/1‑250 (MQ=255)                               cGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTcacca                                                                         <  1:851684/251‑1 (MQ=255)                                          ccGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCt                                                              <  1:515353/251‑1 (MQ=255)                                               tGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCAc                                                         <  1:147129/251‑1 (MQ=255)                                                     tcATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACgtcttc                                                   <  1:176498/250‑1 (MQ=255)                                                           tGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCAt                                             <  1:403288/251‑1 (MQ=255)                                                                gtgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTcacc                                                                          >  1:27339/1‑217 (MQ=255)                                                                gtgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtt                                        >  1:192493/1‑251 (MQ=255)                                                                 tgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttt                                       >  1:132299/1‑251 (MQ=255)                                                                  gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:349524/1‑250 (MQ=255)                                                                  gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:275209/1‑250 (MQ=255)                                                                  gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:615288/1‑250 (MQ=255)                                                                  gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:219693/1‑250 (MQ=255)                                                                    ttATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCTCCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAGCAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtttaac                                    <  1:726568/251‑4 (MQ=255)                                                                     tATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   <  1:656371/251‑1 (MQ=255)                                                                     tATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   <  1:718854/251‑1 (MQ=255)                                                                       tGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  >  1:759226/1‑249 (MQ=255)                                                                       tGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  >  1:329996/1‑249 (MQ=255)                                                                       tGGCATACCAATGCCAACACATCCAAAATCTTCATGCCTCCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  <  1:493914/250‑2 (MQ=255)                                                                            tACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTTATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  <  1:453111/245‑2 (MQ=255)                                                                             aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCAc                                                                                                >  1:623993/1‑182 (MQ=255)                                                                             aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtttaacaccatatt                            >  1:98014/1‑239 (MQ=255)                                                                             aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgccatatt                            >  1:70975/1‑243 (MQ=255)                                                                             aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgccatatt                            >  1:747238/1‑243 (MQ=255)                                                                                aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:128577/251‑1 (MQ=255)                                                                                aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:203677/251‑1 (MQ=255)                                                                                aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGGTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:125781/251‑1 (MQ=255)                                                                                  tGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAAc                      <  1:614694/251‑1 (MQ=255)                                                                                          cATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCCCCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCCCCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccacca              <  1:404859/251‑5 (MQ=255)                                                                                          cATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccacca              >  1:415674/1‑247 (MQ=255)                                                                                            tCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   >  1:615411/1‑228 (MQ=255)                                                                                             ccAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgc           <  1:714588/251‑8 (MQ=255)                                                                                              cAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgc           >  1:725995/1‑243 (MQ=255)                                                                                                      tCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgcccaaaacgt  >  1:527270/1‑235 (MQ=255)                                                                                                                                                                           gCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAAc                      >  1:624742/1‑162 (MQ=255)                                                                                                                                                                                          gTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCgg                                                <  1:73909/121‑1 (MQ=255)                                                                                                                                                                                          gTTCATAGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCg                          >  1:341248/1‑143 (MQ=255)                                                                                                                                                                                                     gCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        >  1:197573/1‑134 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                          CCACCAAGACTTAAGAGCACTCACTGTTACCGGTTTCTCGCCCGGTTGCAGGCCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATCTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATGGCTTGCCCACAACAACTGCGCATCAACAGGCGCACTTTCCTCGGCCAAAACAGGGAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCAGCTTGCACTTCTTCCGGCATGGATTTGACGGCGTATTCGTAACCGCCTCCCAAACCGGAGCGT  >  1/101959‑102310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑