Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	102,150	G→A	H97Y (CAT→TAT)	unknown ←	DNA transposition protein Mup03, A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	102,150	0	G	A	100.0%	199.1 / NA	71	H97Y (CAT→TAT)	unknown	DNA transposition protein Mup03, A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/29);  total (42/29)

GCTATCCATCAAAAGCGGTAGCCACAAACTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGAGCACTCACTGTTACCGGTTTCTCGCCCGGTTGCAGGCCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATCTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATGGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCGGCCAAAACAGGGAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCAGCTTGCACTTCTTCCGGCATGGATTTGACGGCGTATTCGTAACCGCCTCCCAAACCGGAGCGT  >  1/101905‑102310                                                                                                                                                                                                                                                      |                                                                                                                                                                  gCTATCCATTAAAAGCGGTAGCCACAAACTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGTGCACTTACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTg                                                                                                                                                              >  1:355910/1‑251 (MQ=255)       cattaaaaGCGGTAGCCACAAACTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCcaca                                                                                                                                                        >  1:755825/5‑251 (MQ=255)          taaaaGCGGTAGCCACAAACTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCacaa‑ca                                                                                                                                                    >  1:76914/2‑251 (MQ=255)            aaaGCGGTAGCCACAAACTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACaa‑caac                                                                                                                                                  <  1:327418/251‑1 (MQ=255)            aaaGCGGTAGCCACAAACTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACaa‑caac                                                                                                                                                  <  1:685030/251‑1 (MQ=255)                cGGTAGCCACAAACTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTg                                                                                                                                                <  1:174568/249‑1 (MQ=255)                          gaaCTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAgg                                                                                                                                    >  1:731695/2‑251 (MQ=255)                            aCTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGgcg                                                                                                                                  <  1:252497/251‑1 (MQ=255)                                       cGTCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCt                                                                                                                         <  1:671801/249‑1 (MQ=255)                                         tCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAACCAACTGCGCATCAACAGGCGCACTTTCCTcag                                                                                                                      >  1:862269/1‑249 (MQ=255)                                         tCTTTAACTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTcagc                                                                                                                     <  1:735244/251‑4 (MQ=255)                                               aCTTGATACCACCAAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTg                                                                                                                                                <  1:861730/218‑1 (MQ=255)                                                       ccaccaAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGAt                                                                                                       >  1:632240/1‑251 (MQ=255)                                                        caccaAGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGAtt                                                                                                      >  1:630106/1‑251 (MQ=255)                                                             aGACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCa                                                                                                 >  1:41983/1‑251 (MQ=255)                                                              gACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCa                                                                                                 >  1:841580/1‑250 (MQ=255)                                                              gACTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGGCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCAAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCa                                                                                                 >  1:835758/1‑250 (MQ=255)                                                               aCTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCAc                                                                                                <  1:150007/250‑1 (MQ=255)                                                               aCTTAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCAc                                                                                                <  1:619089/250‑1 (MQ=255)                                                                  tAAGTGCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCa                                                                                            >  1:70006/1‑251 (MQ=255)                                                                    agtgCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCa                                                                                            >  1:67207/4‑249 (MQ=255)                                                                     gtgCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACtt                                                                                         >  1:551902/3‑251 (MQ=255)                                                                      tgCACTCACCGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACttt                                                                                        <  1:135550/250‑1 (MQ=255)                                                                          ctcaccGTGACCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGcc                                                                                    <  1:494922/251‑1 (MQ=255)                                                                            caccgtgaCCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACaa‑caac                                                                                                                                                  >  1:21684/8‑187 (MQ=255)                                                                            caccgtgaCCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCaa                                                                                  >  1:98421/8‑251 (MQ=255)                                                                              ccgtgaCCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAAc                                                                                                               >  1:829902/6‑220 (MQ=255)                                                                              ccgtgaCCGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGt                                                                                >  1:359769/6‑251 (MQ=255)                                                                                    ccGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTcac                                                                           >  1:215209/1‑250 (MQ=255)                                                                                     cGGTTTCTCGCCCGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTcacca                                                                         <  1:851684/251‑1 (MQ=255)                                                                                                ccGGTTGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCt                                                              <  1:515353/251‑1 (MQ=255)                                                                                                     tGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTc                                                                                                                        >  1:322600/1‑188 (MQ=255)                                                                                                     tGCAGGTCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCAc                                                         <  1:147129/251‑1 (MQ=255)                                                                                                           tcATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACgtcttc                                                   <  1:176498/250‑1 (MQ=255)                                                                                                                 tGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCAt                                             <  1:403288/251‑1 (MQ=255)                                                                                                                  ggTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAAc                                                                                                               <  1:781216/184‑1 (MQ=255)                                                                                                                      gtgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTcacc                                                                          >  1:27339/1‑217 (MQ=255)                                                                                                                      gtgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtt                                        >  1:192493/1‑251 (MQ=255)                                                                                                                       tgtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttt                                       >  1:132299/1‑251 (MQ=255)                                                                                                                        gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:349524/1‑250 (MQ=255)                                                                                                                        gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:275209/1‑250 (MQ=255)                                                                                                                        gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:219693/1‑250 (MQ=255)                                                                                                                        gtTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAttta                                      >  1:615288/1‑250 (MQ=255)                                                                                                                          ttATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCTCCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAGCAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtttaac                                    <  1:726568/251‑4 (MQ=255)                                                                                                                           tATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   <  1:656371/251‑1 (MQ=255)                                                                                                                           tATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   <  1:718854/251‑1 (MQ=255)                                                                                                                             tGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACaa‑caac                                                                                                                                                  >  1:470490/1‑138 (MQ=255)                                                                                                                             tGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  >  1:759226/1‑249 (MQ=255)                                                                                                                             tGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  >  1:329996/1‑249 (MQ=255)                                                                                                                             tGGCATACCAATGCCAACACATCCAAAATCTTCATGCCTCCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  <  1:493914/250‑2 (MQ=255)                                                                                                                                 aTACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGccc                                                                                                                                                           >  1:256356/1‑126 (MQ=255)                                                                                                                                  tACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTTATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgc                                  <  1:453111/245‑2 (MQ=255)                                                                                                                                  tACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACaa‑caac                                                                                                                                                  >  1:175101/1‑133 (MQ=255)                                                                                                                                   aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCAc                                                                                                >  1:623993/1‑182 (MQ=255)                                                                                                                                   aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGAtttaacaccatatt                            >  1:98014/1‑239 (MQ=255)                                                                                                                                   aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgccatatt                            >  1:70975/1‑243 (MQ=255)                                                                                                                                   aCCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACgccatatt                            >  1:747238/1‑243 (MQ=255)                                                                                                                                      aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:203677/251‑1 (MQ=255)                                                                                                                                      aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:128577/251‑1 (MQ=255)                                                                                                                                      aaTGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGGTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTa                        <  1:125781/251‑1 (MQ=255)                                                                                                                                        tGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAAc                      <  1:614694/251‑1 (MQ=255)                                                                                                                                                cATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCCCCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCCCCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccacca              <  1:404859/251‑5 (MQ=255)                                                                                                                                                cATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccacca              >  1:415674/1‑247 (MQ=255)                                                                                                                                                  tCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACg                                   >  1:615411/1‑228 (MQ=255)                                                                                                                                                   ccAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgc           <  1:714588/251‑8 (MQ=255)                                                                                                                                                    cAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgc           >  1:725995/1‑243 (MQ=255)                                                                                                                                                            tCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAACCGccaccacgcccaaaacgt  >  1:527270/1‑235 (MQ=255)                                                                                                                                                                    ccGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATTTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACa                                                                                                                                      >  1:741212/1‑111 (MQ=255)                                                                                                                                                                                                                                 gCTTTGCAGTGCCTTGTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCGTAAc                      >  1:624742/1‑162 (MQ=255)                                                                                                                                                                                                                                                gTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCgg                                                <  1:73909/121‑1 (MQ=255)                                                                                                                                                                                                                                                gTTCATAGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCAGCCAAAACAGGCAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCGGCTTGCACGTCTTCCGGCATGGATTTAACGCCATATTCg                          >  1:341248/1‑143 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                  GCTATCCATCAAAAGCGGTAGCCACAAACTACGATCAGCGTCTTTAACTTGATACCACCAAGACTTAAGAGCACTCACTGTTACCGGTTTCTCGCCCGGTTGCAGGCCATCTTGGTTGTGTTTATGGCATACCAATGCCAACGCATCCAAAATCTTCATGCCACCGTTGACCAATTCAGCCACCGCAAACATAATGCCAAGCTTCATCTGCGCTTTTTGTTGTTGCTTTGCAGTGCCTTGTTCATGGCTTGCCCACAA‑CAACTGCGCATCAACAGGCGCACTTTCCTCGGCCAAAACAGGGAGATTTTTCACCGCACTTTTGCCAAGTTTCACCACGATTTCAGCTTGCACTTCTTCCGGCATGGATTTGACGGCGTATTCGTAACCGCCTCCCAAACCGGAGCGT  >  1/101905‑102310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑