Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	94,990	G→A	G126G (GGC→GGT)	unknown ←	Phage protein Mup37, K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	94,990	0	G	A	97.7%	112.8 / NA	44	G126G (GGC→GGT)	unknown	Phage protein Mup37, K
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/26);  minor base T (0/1);  total (17/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ATGCACCACCTGCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAGTAGATGCCATCCATCGCGCCCCAACGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACTTGGGTGAGCGGATAACGATGCAATCCGGC  >  1/94766‑95082                                                                                                                                                                                                                                 |                                                                                             aTGCACCACCTGCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGGTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCa                                                                     <  1:436123/250‑1 (MQ=255) aTGCACCACCTGCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGCGCCGGTTTGATAAGCCATTATTCGATTTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAAAACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGGCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCAt                                                                    >  1:549416/1‑251 (MQ=255)    caccacCTGCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATcc                                                                  <  1:735221/250‑1 (MQ=255)      ccaccTGCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATcc                                                                  >  1:480363/1‑248 (MQ=255)           tGCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTTATAAGCCATTTTTCGACCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACAAGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAAAGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGAtt                                                                       >  1:417540/1‑238 (MQ=255)           tGCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCgat                                                          >  1:805030/1‑251 (MQ=255)           tGCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCgat                                                          <  1:5771/251‑1 (MQ=255)            gCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCgatg                                                         >  1:81266/1‑251 (MQ=255)             ccAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCgatg                                                         <  1:481836/250‑1 (MQ=255)                             ataaAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGTAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTag                                        <  1:449953/251‑1 (MQ=255)                             ataaAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTag                                        <  1:681093/251‑1 (MQ=255)                             ataaAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTag                                        <  1:826507/251‑1 (MQ=255)                                aaaTCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGc                                     <  1:551726/251‑1 (MQ=255)                                aaaTCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGc                                     <  1:216726/251‑1 (MQ=255)                                          aTTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCAcctgag                           <  1:866628/251‑6 (MQ=255)                                            ttttCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCAcctgagtg                         <  1:122117/251‑8 (MQ=255)                                               tCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGc                      <  1:352593/251‑1 (MQ=255)                                                            cGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATTCCATCAAACGCGCCCCAGCGATTCATCCCTTGAGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgat            <  1:824852/248‑6 (MQ=255)                                                            cGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgat            <  1:8948/248‑6 (MQ=255)                                                              atatTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCgggtagtgattatta       >  1:41829/1‑238 (MQ=255)                                                              atatTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgattatt        <  1:38781/250‑10 (MQ=255)                                                              atatTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgattatt        <  1:312299/250‑10 (MQ=255)                                                              atatTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgattatt        <  1:771669/250‑10 (MQ=255)                                                              atatTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagggatttttt       >  1:193671/1‑241 (MQ=255)                                                                    ccGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgattattaattgc  <  1:11485/250‑16 (MQ=255)                                                                               ggTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgat            >  1:482447/1‑224 (MQ=255)                                                                                         gCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgattatt        >  1:108927/1‑214 (MQ=255)                                                                                         gCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgat            >  1:846927/1‑214 (MQ=255)                                                                                          ccATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCgatga                                                        >  1:590140/1‑174 (MQ=255)                                                                                          ccATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCAc                                <  1:887593/198‑1 (MQ=255)                                                                                            attattCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgat            <  1:573809/216‑6 (MQ=255)                                                                                               attCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATAccatcc                                                                                        <  1:772091/137‑1 (MQ=255)                                                                                                             gCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgattatt        >  1:110133/1‑194 (MQ=255)                                                                                                                aaaTAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtga             >  1:242384/1‑191 (MQ=255)                                                                                                                                   cccGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTc                                   >  1:226177/1‑154 (MQ=255)                                                                                                                                     cGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCAcc                               <  1:493050/156‑2 (MQ=255)                                                                                                                                                gtTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCAcc                               <  1:769624/145‑2 (MQ=255)                                                                                                                                                        cGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCga                                                           >  1:400939/1‑109 (MQ=255)                                                                                                                                                        cGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgattatt        >  1:240912/1‑151 (MQ=255)                                                                                                                                                              gCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATagtgat            >  1:477525/1‑145 (MQ=255)                                                                                                                                                              gCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACCTGAGTGAGCGGATag                <  1:136340/146‑2 (MQ=255)                                                                                                                                                                                                       gtgCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCAcc                               <  1:267039/90‑2 (MQ=255)                                                                                                                                                                                                        tgCCGATACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCAcctgagtg                         <  1:383959/95‑8 (MQ=255)                                                                                                                                                                                                             aTACCGGGCAGCCAATAAATACCATCCATCGCGCCCCAGCGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCAcctgagtg                         <  1:455963/90‑8 (MQ=255)                                                                                                                                                                                                                                 |                                                                                             ATGCACCACCTGCCAATTAAGCGGTTTAATAAAATCGGCTAATTTTTCAAAAAGTGCAGCGATATTGCCGGCGGTGCCGGTTTGATAAGCCATTATTCGATCTCCATTGCAAAATAACTGGTAGTTGTCACCCGATAAGCGTCGTTAAACACGATAAGCTTACGTCCATCCTTGGTTTTAATCGTATCCAATGCCAATGTGCCGATACCGGGCAGCCAGTAGATGCCATCCATCGCGCCCCAACGATTCATCCCTTGCGATGATTCGGCGGTGGAGTAGAGCTCCACTTGGGTGAGCGGATAACGATGCAATCCGGC  >  1/94766‑95082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑