Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,978,545	T→C	S237S (AGT→AGC)	unknown →	Predicted transcriptional regulator of the myo‑inositol catabolic operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,978,545	0	T	C	100.0%	225.9 / NA	78	S237S (AGT→AGC)	unknown	Predicted transcriptional regulator of the myo‑inositol catabolic operon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/33);  total (45/33)

CGTTGCCTGTTATCTCAACTACGCTCTCCATCACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGTGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTA  >  1/1978305‑1978787                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   cGTTGCCTGTTATCTCAACTACGCTCTCCATCACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCtt                                                                                                                                                                                                                                          <  1:765665/251‑1 (MQ=255)           tATCTCAACTACGCTCTCCATCACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATaaa                                                                                                                                                                                                                                >  1:518937/1‑251 (MQ=255)           tATCTCAACTACGCTCTCCATCACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATaaa                                                                                                                                                                                                                                >  1:636531/1‑251 (MQ=255)              ctcAACTACGCTCTCCATCACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAaaga                                                                                                                                                                                                                             >  1:206803/1‑251 (MQ=255)                   cTACGCTCTCCATCACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAgcgc                                                                                                                                                                                                                        <  1:174264/251‑1 (MQ=255)                        ctctCCATCACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGgtg                                                                                                                                                                                                                   <  1:276429/251‑1 (MQ=255)                          ctcCATCACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGtt                                                                                                                                                                                                                 >  1:227579/1‑251 (MQ=255)                                cACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGa                                                                                                                                                                                                             <  1:76643/249‑1 (MQ=255)                                  cTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGAttt                                                                                                                                                                                                          >  1:619215/1‑250 (MQ=255)                                    tAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTATGATAAAAGAAGCGCAGGTGTTGGGATTTCGt                                                                                                                                                                                                       >  1:84797/1‑251 (MQ=255)                                                         ttaatGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACg                                                                                                                                                                                  <  1:323536/251‑1 (MQ=255)                                                             tGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTggg                                                                                                                                                                                                              >  1:29271/1‑219 (MQ=255)                                                              ggCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAAc                                                                                                                                                                                         >  1:230038/1‑239 (MQ=255)                                                              ggCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGt                                                                                                                                                                             >  1:16795/1‑251 (MQ=255)                                                                cTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTc                                                                                                                                                                           <  1:832793/251‑1 (MQ=255)                                                                   gggCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCaaa                                                                                                                                                                        >  1:197058/1‑251 (MQ=255)                                                                              ttGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGAtt                                                                                                                                                             >  1:244305/1‑251 (MQ=255)                                                                              ttGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGAtt                                                                                                                                                             >  1:874972/1‑251 (MQ=255)                                                                              ttGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGAtt                                                                                                                                                             >  1:771564/1‑251 (MQ=255)                                                                              ttGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGAtt                                                                                                                                                             >  1:758877/1‑251 (MQ=255)                                                                              ttGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCC‑AAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAac                                                                                                                                                                       >  1:225271/1‑240 (MQ=255)                                                                                  tgaACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGc                                                                                                                                                         <  1:816096/251‑1 (MQ=255)                                                                                  ggaccgatattccgatgttcttgggggCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATtgtg                                                                                                                                                          >  1:104757/26‑250 (MQ=255)                                                                                   gaACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATtgtg                                                                                                                                                          >  1:895585/1‑249 (MQ=255)                                                                                        gCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACg                                                                                                                                                                                  >  1:480177/1‑220 (MQ=255)                                                                                        gCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCg                                                                                                                                                                >  1:465643/1‑238 (MQ=255)                                                                                         cTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAggg                                                                                                                                                    <  1:84504/249‑1 (MQ=255)                                                                                         cTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGc                                                                                                                                                   <  1:753812/250‑1 (MQ=255)                                                                                                 ggTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAacac                                                                                                                                                                     >  1:265659/1‑224 (MQ=255)                                                                                                 ggTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGc                                                                                                                                                   <  1:126526/242‑1 (MQ=255)                                                                                                 ggTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGgaaaaag                                                                                                                                          >  1:343784/1‑251 (MQ=255)                                                                                                               aTGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCg                                                                                                                                                                <  1:532009/215‑1 (MQ=255)                                                                                                               aTGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAAca                                                                                                                            >  1:790837/1‑251 (MQ=255)                                                                                                                   ggtggtTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGgtg                                                                                                                                                                                                                   >  1:656654/1‑160 (MQ=255)                                                                                                                   ggtggtTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAggg                                                                                                                                                    <  1:69896/223‑1 (MQ=255)                                                                                                                           ccATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGc                                                                                                                                                                                              <  1:601922/173‑1 (MQ=255)                                                                                                                                 gTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATg                                                                                                           >  1:764633/1‑250 (MQ=255)                                                                                                                                   ttCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGccc                                                                                                        <  1:761032/251‑1 (MQ=255)                                                                                                                                       cGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGaa                                                                                                    >  1:526022/1‑251 (MQ=255)                                                                                                                                             aTGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACgggg                                                                                               >  1:776462/1‑250 (MQ=255)                                                                                                                                             aTGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGc                                                                                              <  1:227288/251‑1 (MQ=255)                                                                                                                                                  gcggAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCAtttt                                                                                         >  1:312999/1‑251 (MQ=255)                                                                                                                                                   cggAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAACCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCAtttt                                                                                         <  1:552369/250‑1 (MQ=255)                                                                                                                                                      aaaCCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACCTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGt                                                                                     <  1:427227/251‑1 (MQ=255)                                                                                                                                                         ccGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGa                                                                                  >  1:850798/1‑251 (MQ=255)                                                                                                                                                          cGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGa                                                                                                                                                               >  1:389579/1‑173 (MQ=255)                                                                                                                                                          cGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAggg                                                                                                                                                    >  1:619526/1‑184 (MQ=255)                                                                                                                                                          cGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAggg                                                                                                                                                    >  1:371063/1‑184 (MQ=255)                                                                                                                                                               atatTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAgcgc                                                                                                                                                                                                                        <  1:820975/111‑1 (MQ=255)                                                                                                                                                               atatTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAac                                                                                                                                                                       >  1:464516/1‑160 (MQ=255)                                                                                                                                                               atatTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGtta                                                                            >  1:584285/1‑251 (MQ=255)                                                                                                                                                                tattatGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGttat                                                                           <  1:562151/251‑1 (MQ=255)                                                                                                                                                                tattatGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGttat                                                                           <  1:124144/251‑1 (MQ=255)                                                                                                                                                                 attatGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTAc                                                                                       <  1:640828/238‑1 (MQ=255)                                                                                                                                                                 attatGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTAc                                                                                       <  1:69342/238‑1 (MQ=255)                                                                                                                                                                 attatGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGttatt                                                                          >  1:18383/1‑251 (MQ=255)                                                                                                                                                                   tatGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGAttt                                                                                                                                                                                                          <  1:830124/121‑1 (MQ=255)                                                                                                                                                                   tatGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTCGCCTTCAGCGTTATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGCTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTaa                                                                        >  1:447762/1‑251 (MQ=255)                                                                                                                                                                    atGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGc                                                                                              >  1:95821/1‑228 (MQ=255)                                                                                                                                                                    atGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTaa                                                                        >  1:482219/1‑250 (MQ=255)                                                                                                                                                                    atGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAAc                                                                       <  1:381294/251‑1 (MQ=255)                                                                                                                                                                      gAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAacac                                                                                                                                                                     >  1:616937/1‑155 (MQ=255)                                                                                                                                                                              gTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCa                                                             >  1:799322/1‑251 (MQ=255)                                                                                                                                                                               tGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCaa                                                            <  1:285206/251‑1 (MQ=255)                                                                                                                                                                                     aaaaGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGaaaaagaaaaag                                                                                                                                    <  1:812935/173‑1 (MQ=255)                                                                                                                                                                                     aaaaGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTa                                                      >  1:135426/1‑251 (MQ=255)                                                                                                                                                                                          gCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATc                                                   <  1:229458/249‑1 (MQ=255)                                                                                                                                                                                                 gCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGCCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGCTTAATCAGtctt                                             >  1:17014/1‑248 (MQ=255)                                                                                                                                                                                                  cAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGt                                         <  1:470951/251‑1 (MQ=255)                                                                                                                                                                                                   aaaTTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGt                                         <  1:755555/250‑1 (MQ=255)                                                                                                                                                                                                   aaaTTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTa                                        >  1:273223/1‑251 (MQ=255)                                                                                                                                                                                                    aaTTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTAt                                       <  1:390562/251‑1 (MQ=255)                                                                                                                                                                                                         ccATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGttatt                                                                          <  1:546997/211‑1 (MQ=255)                                                                                                                                                                                                           aTCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGa                                                                   >  1:92296/1‑216 (MQ=255)                                                                                                                                                                                                           aTCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAg                                                 <  1:380977/234‑1 (MQ=255)                                                                                                                                                                                                                                 cGCTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTc          <  1:310284/251‑1 (MQ=255)                                                                                                                                                                                                                                   cTACTTGCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAg                                                 >  1:108005/1‑210 (MQ=255)                                                                                                                                                                                                                                         gCCTTCAGCGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTa  >  1:773112/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   CGTTGCCTGTTATCTCAACTACGCTCTCCATCACTTAGATTGCAGTTCCTTTTTAATTAATGGCTTGGGCGGGATGTTTGATGAACAGCTCAATCAGGTTAAAGAAGGCGATGTGGTGGTTGCCATTAGTTTCTCGCCTTATGCGGCGGAAACCGTCAATATTATGAATACCAGTGCCAAAAAAGGCATTAAGCAAATTGCCATCACCGACAGCCAAATCAGCCCGCTACTTGCCTTCAGTGATATTGCTTTTGTGATAAAAGAAGCGCAGGTGTTGGGATTTCGTTCCCAATGCTCAACGATGACGTTGGTTCAAACACTGGCGATTGTGCTAGGGCTGGAAAAAGAAAAAGAGAAAACAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTA  >  1/1978305‑1978787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑