Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,910,519	T→G	intergenic (‑28/‑190)	unknown ← / → unknown	RNA chaperone ProQ/Inner membrane protein YebS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,910,519	0	T	G	100.0%	90.4 / NA	31	intergenic (‑28/‑190)	unknown/unknown	RNA chaperone ProQ/Inner membrane protein YebS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/15);  total (16/15)

GTGTAGGTAACGCCAGTTTGAAGTATATTGACGCAAGGCGTGACGGAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAATTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCGCACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTC  >  1/1910276‑1910763                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     gtgtAGGTAACGCCAGTTTGAAGTATATTGACGCAAGGCGTGACGGAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGaaaa                                                                                                                                                                                                                                               >  1:558242/1‑251 (MQ=255)                 tttGAAGTATATTGACGCAAGGCGTGACGGAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGttt                                                                                                                                                                                                                                 <  1:96590/249‑1 (MQ=255)                  ttGAAGTATATTGACGCAAGGCGTGACGGAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGc                                                                                                                                                                                                                              <  1:378185/251‑1 (MQ=255)                     aaGTATATTGACGCAAGGCGTGACGGAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCtt                                                                                                                                                                                                                            <  1:722860/250‑1 (MQ=255)                              gACGCAAGGCGTGACGGAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGaa                                                                                                                                                                                                                  >  1:843400/1‑251 (MQ=255)                                       cGTGACGGAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGAc                                                                                                                                                                                                          <  1:403249/250‑1 (MQ=255)                                              gAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCGTTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATtaata                                                                                                                                                                                                  >  1:428008/1‑251 (MQ=255)                                              gAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACtt                                                                                                                                                                                                                                           >  1:101750/1‑210 (MQ=255)                                                 ttGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAatc                                                                                                                                                                                               <  1:611747/251‑1 (MQ=255)                                                 ttGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAatc                                                                                                                                                                                               <  1:469365/251‑1 (MQ=255)                                                                            tttCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAAtt                                                                                                                                                                     >  1:28225/1‑250 (MQ=255)                                                                                              aTCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTAc                                                                                                                                                                                        >  1:364450/1‑213 (MQ=255)                                                                                               tCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGc                                                                                                                                                                                                                              >  1:525521/1‑174 (MQ=255)                                                                                                    aaacaaacCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTc                                                                                                                                            <  1:35909/251‑1 (MQ=255)                                                                                                                                 cccccTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTaaaa                                                                                                               >  1:881364/1‑251 (MQ=255)                                                                                                                                    ccTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACg                                                                                                            >  1:157293/1‑251 (MQ=255)                                                                                                                                         aTAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCAt                                                                                                                                                                                                                                      >  1:443686/1‑124 (MQ=255)                                                                                                                                            gAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAATAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTc                                                                                                                                            <  1:598252/211‑1 (MQ=255)                                                                                                                                            gAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTAc                                                                                                                                                                                        <  1:877040/167‑1 (MQ=255)                                                                                                                                            gAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGa                                                                                                                                                                                     >  1:191204/1‑170 (MQ=255)                                                                                                                                              aaaaCATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAg                                                                                                  >  1:809775/1‑251 (MQ=255)                                                                                                                                                       cGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAAt                                                                                          <  1:604096/250‑1 (MQ=255)                                                                                                                                                       cGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAAt                                                                                          <  1:293081/250‑1 (MQ=255)                                                                                                                                                                             gCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTg                                                                                                                                                                                      <  1:358982/136‑1 (MQ=255)                                                                                                                                                                                       ctTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACttt                                                                                                                                                                                                                       >  1:542487/1‑93 (MQ=255)                                                                                                                                                                                       ctTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGAtt                                                          >  1:315192/1‑250 (MQ=255)                                                                                                                                                                                                            gTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCtt                                                                                                                                                                                                                            <  1:565300/67‑1 (MQ=255)                                                                                                                                                                                                                ttCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAAtt                                <  1:312416/251‑1 (MQ=255)                                                                                                                                                                                                                          gCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATAc                                                                                                    >  1:374209/1‑173 (MQ=255)                                                                                                                                                                                                                                         gATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAata                                                                          >  1:313256/1‑184 (MQ=255)                                                                                                                                                                                                                                              taataaGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCtcgttc  <  1:67662/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     GTGTAGGTAACGCCAGTTTGAAGTATATTGACGCAAGGCGTGACGGAGTTGGGTTTTGCTTACGCGCTCGTCATCTTTCAGCGCTTCGCTTAAATCCTGAAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAATTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCGCACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTC  >  1/1910276‑1910763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑