| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,894,195 | A→C | R243S (AGA→AGC) | unknown → | Hydrogenase‑4 component I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,894,195 | 0 | A | C | 98.5% | 192.7 / NA | 67 | R243S (AGA→AGC) | unknown | Hydrogenase‑4 component I |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (36/29); total (36/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCAAAAACTGAAAGGCAAACAGGAAAAAGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGAGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCAGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGCACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGCTTTGTCGAAAACGAAGAACAAGTGCAGGA > 1/1893954‑1894442 | aTCAAAAACTGAAAGGCAAACAGGAAAAAGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGt > 1:249783/1‑250 (MQ=255) gCAAACAGGAAAAAGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGc < 1:112517/250‑1 (MQ=255) gCAAACAGGAAAAAGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGc < 1:513489/250‑1 (MQ=255) ggAAAAAGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTa > 1:736398/1‑251 (MQ=255) gAAAAAGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAg < 1:867442/248‑1 (MQ=255) gAAAAAGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTa < 1:36713/250‑1 (MQ=255) gAAAAAGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTa < 1:148951/250‑1 (MQ=255) aaGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGccg > 1:697356/1‑249 (MQ=255) tCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGCTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGCTTTCgg > 1:340072/1‑251 (MQ=255) ccGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCggg < 1:525139/251‑1 (MQ=255) ccGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGtt < 1:773771/251‑1 (MQ=255) ccGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGtt < 1:284370/251‑1 (MQ=255) ccAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGTTGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑c < 1:528512/251‑1 (MQ=255) tGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTTGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACAt < 1:618368/251‑1 (MQ=255) cGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATtc < 1:632478/251‑1 (MQ=255) tttCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTcc < 1:49479/251‑1 (MQ=255) ccGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAAc < 1:141316/183‑1 (MQ=255) ccGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCggg > 1:494258/1‑251 (MQ=255) ggATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAGT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTc > 1:440126/1‑207 (MQ=255) ggATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCggggc > 1:529437/1‑246 (MQ=255) aTTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCtgtg < 1:71214/251‑1 (MQ=255) ttGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCggggcccgtgg > 1:501128/1‑242 (MQ=255) ttGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTg‑cc < 1:593532/251‑1 (MQ=255) gCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAgggg > 1:718415/1‑236 (MQ=255) tATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGccg > 1:495921/1‑191 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGaaa < 1:854750/156‑1 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGc > 1:268532/1‑186 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCgggg < 1:603928/197‑1 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑c > 1:532893/1‑209 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑c > 1:197950/1‑209 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAgggg < 1:756692/228‑1 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTg‑cccc‑cccccagt > 1:504547/1‑246 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTg‑cc < 1:866842/241‑1 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAAtt > 1:787513/1‑251 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCCTTg‑cccg‑cccccatt > 1:147568/1‑246 (MQ=255) gCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCACCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTcc > 1:3485/1‑220 (MQ=255) aCGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGt > 1:104634/1‑175 (MQ=255) cTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTg‑cccg‑cc > 1:704483/1‑230 (MQ=255) cgtcgtCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATggg < 1:561533/251‑1 (MQ=255) tcgtcTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTGTG‑GCCG‑CCC‑GGAttttaggtttggtaaaggggg > 1:530841/1‑233 (MQ=255) gtcTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATggg > 1:790578/1‑247 (MQ=255) gTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAg < 1:709401/195‑1 (MQ=255) gTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATggg > 1:691621/1‑238 (MQ=255) gTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTGTG‑GCCG‑CCC‑GCATTTTC‑G‑TTT‑TGGTAATgggcgggggcaggag > 1:44150/1‑242 (MQ=255) ttATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTGAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGCACA‑GGAcc < 1:441037/250‑1 (MQ=255) aTCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGc > 1:844445/1‑153 (MQ=255) tCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGCACA‑GGACCCGcc < 1:259864/251‑1 (MQ=255) cccgcaaataattttagggggatgatGTGGGAAGATGA‑GGGGGGCCGTTGGGGTGTGCGTTTGGGGAAATCCCGGGAAGGGGAAGGGGATCCGCGCGTGAGAAAAAT‑CGACACCCGTCGGCATGCAATTAGTATGCCGTTTGGGGGGTAAGTTATGGG‑CTTACATTCCCCCGGGGGGGGGGGTCCTGTG‑CCCG‑CCC‑GAATTTCC‑G‑TTG‑GGATAAGGGGGGGGCTCA‑GGACCCGCCCCTACAAAAGCAActc < 1:100806/231‑1 (MQ=255) atTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTa < 1:779778/122‑1 (MQ=255) atTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTGTG‑GCCG‑CCCGGAATTTA‑‑G‑TTT‑TGGTAAAGGGGGGGGACA‑GGAcccccccccccaaaaaacaatcttgttgtttgg > 1:28201/1‑226 (MQ=255) atTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTGTG‑CCCG‑CCC‑CGATTTTC‑G‑TTT‑TGGTAAATGGCGGGGACA‑GGAcccccccccccaaaaaacaacccgtttctttga > 1:734981/1‑226 (MQ=255) cgagggtgTCGGAGGATGACGGCGTGCGGTTC‑GGTGTGCGTTGGGGGAAATCCGTGGGACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGACACCCGTCTGCATGCCTTTTGTAGGCCTTTTGCGGGGTAAGTTATTGG‑CTTACTTTCTCCGGTAGGGGGGGTTCCTGTG‑CCCG‑CCC‑GATTTTAC‑G‑TGT‑GGATAAGGGGGGGGCAGA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGc < 1:901129/245‑1 (MQ=255) atgTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGCCCTGTG‑CCCGCCCC‑GAA‑TTAC‑GCTTT‑GG‑TAAAGGGGGGGCCGA‑GGGCCCCCCCCCCCAAAAACAAATCTGTTGCTTAGATGGaaaaaagtaa > 1:483677/1‑244 (MQ=255) gATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGAt < 1:487490/179‑1 (MQ=255) gTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCCGTG‑GCCGCCCC‑CAATTT‑C‑G‑TTT‑TGGTAAAAGGGGGGCGCA‑GGACCCCCCCCCCCAAAAAAAACACTGTTTCTTTGATGTAAAAAAGTAAGGACggagcctttctctt > 1:861987/1‑238 (MQ=255) gggcggttgggtgggctttggggggAATCCCTGGAGGGGGAGGCGGACCCGCGCCGGAG‑CAAATCCGCCACCCGTCTGCTTCCCTTTAGTTTGCTGTTTGGGGGTTAATTTTTTGG‑CTTCCTTTCCCCCGGGGGGGGGGGTCCTGCC‑CCCG‑CCC‑AATTTTCC‑T‑TTT‑GAATAAGGGGGGGGCACA‑GGCCCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGaccac < 1:239164/229‑1 (MQ=255) ccGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCCGTG‑CCCC‑CCC‑CCATTTTCGG‑TTT‑GGTAAATGGG‑GGGCACA‑AGACCCCCCCCCCCAAAAACAACTCTTTTGCTTTGATGTAAAGCAGGAAGGACGGATCATTaccacccca > 1:42518/1‑248 (MQ=255) ccGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAAAGGGCGGGCACA‑GGACCCCCCCCCCCAAAAACAAACCTGTTGCTTAGATGTAAAACAGTAAGGACGGATCATGaccacccta > 1:513918/1‑248 (MQ=255) gtgtGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGCACA‑g > 1:489523/1‑175 (MQ=255) gtgtGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCCGTGCCCCC‑CCC‑GAATTT‑C‑G‑TTT‑GGGTAATTGGCGGGGACC‑GGACCCCCCCCCCCAAAAACAACTCTTATTCTTAGATGTAAAGCAGGAAAGACGGATCATGAACACCCTAatctttt > 1:756094/1‑249 (MQ=255) gCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTa < 1:508865/77‑1 (MQ=255) gCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTGAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCCGTG‑CCCG‑CCC‑GCATTTTC‑G‑TTT‑TGGTAATGGGGGGGCACA‑AGAACCCCCCCCACAAAAAAAAACCTGTTTCTTTGATGTAAAACAGGAAAGAAGGATCATTAACACCCTAatcttttat > 1:536486/1‑249 (MQ=255) gCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTcc > 1:140348/1‑114 (MQ=255) gCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCCGTG‑CCCC‑CCC‑CGATTTTC‑G‑TTT‑TGTTTATGGGCGGGCACA‑GGACCCGCCCCCACCAAAACAAATCTGTTTCTTTGATGTAAAACAGGAAGGACGGATCacctgctctttttcacaactcc > 1:301716/1‑229 (MQ=255) gTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑t < 1:259133/148‑1 (MQ=255) gTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGGCCTGTG‑CCCG‑CCC‑CGATTTAC‑G‑GTT‑TGATAATGGGGGGGCACA‑GGACCCCCCCCCCCCAAAACAACACTGTTGGTTTGATGTAAAAGAGGAAAGACGGAACATTAACACCGCAaacttctatcttt > 1:742703/1‑250 (MQ=255) tACCTGGAACGGGAAGCGGATCCGCGCCTGAGCGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCCGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑t < 1:292450/137‑1 (MQ=255) ggagaAAT‑CGTCACCCGTCTGCATGCAATTAGTAGGCCTTTTTGGGGGT‑AGTTTTTGGTCTTACTTTCCCCGGGGGGGGGGGGTCGTGCC‑CCCG‑‑CC‑AAATTTTG‑G‑TTTGGGAT‑AGGGGGGGGCAAAGGGCCCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGCTTTGTCGAAAACGAAGAACAAGTGCAGGa < 1:210994/249‑1 (MQ=255) | ATCAAAAACTGAAAGGCAAACAGGAAAAAGCCGATCCGAATGCCGACGCCAAATTGCGTTTTCCGGGCATTCCGCTGGATTTGCGTATCAGCTTAGAACGGGAGGCTCGTCGTCTTGCCGGTTATCGCCAGGGTGGCAATATCGCCAATCAATTTATGGCGATGATGTCGGAAGATGACAGCGTGCCGTTC‑GGTGTGCGTTTAGGGGAATACCTGGAACGGGAAGCGGATCCGCGCCTGAGAGAAAT‑CGTCAACCGTCTGCATGCCATTAGTATGCAGTTTTCGGGGTAAGTTATTGG‑CTTACATTCTCCCGTAGGGGCGGGTCCTGTG‑CCCG‑CCC‑GAATTTAC‑G‑TTT‑GGATAATGGGCGGGCACA‑GGACCCGCCCCTACAAAAGCAACTCTGTTGCTTAGATGTAAAGCAGTAAGGACGTATCATGACCACCGTAATCTTCTATCTTCTCAACGAACGCTTTGTCGAAAACGAAGAACAAGTGCAGGA > 1/1893954‑1894442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |