Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,811,242	T→C	D193D (GAT→GAC)	unknown →	Cysteine desulfurase (EC 2.8.1.7) => sulfur transfer pathway protein CsdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,811,242	0	T	C	100.0%	189.2 / NA	66	D193D (GAT→GAC)	unknown	Cysteine desulfurase (EC 2.8.1.7) => sulfur transfer pathway protein CsdA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/33);  total (33/33)

TCATCACGCCAATTTTGTGACCTGGCATGAAATTGCCGAAAAAAGCGGCGCTAAAATTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGATGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCGCCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGC  >  1/1811005‑1811484                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   tcatcaCGCCAATTTTGTGACCTGGCATGAAATTGCCGAAAAAAGCGGCGCTAAAATTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGa                                                                                                                                                                                                                                       >  1:651952/1‑251 (MQ=255)         ccAATTTTGTGACCTGGCATGAAATTGCCGAAAAAAGCGGCGCTAAAATTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCcac                                                                                                                                                                                                                               <  1:652331/251‑1 (MQ=255)                  tgACCTGGCATGAAATTGCCGAAAAAAGCGGCGCTAAAATTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAAt                                                                                                                                                                                                                       <  1:552165/250‑1 (MQ=255)                        ggCATGAAATTGCCGAAAAAAGCGGCGCTAAAATTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTg                                                                                                                                                                                                                >  1:349166/1‑251 (MQ=255)                                                gcgcTAAAATTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTAt                                                                                                                                                                                         <  1:134441/250‑1 (MQ=255)                                                 cgcTAAAATTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATcg                                                                                                                                                                                       <  1:884304/251‑1 (MQ=255)                                                       aaTTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTc                                                                                                                                                                                                       >  1:579350/1‑229 (MQ=255)                                                       aaTTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGtttt                                                                                                                                                                                 >  1:408410/1‑251 (MQ=255)                                                                     ccATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAAtt                                                                                                                                                                   <  1:865799/251‑1 (MQ=255)                                                                           gCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATgg                                                                                                                                                              <  1:881483/250‑1 (MQ=255)                                                                                gatTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGa                                                                                                                                                         >  1:875426/1‑250 (MQ=255)                                                                                    ggTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATc                                                                                                                                                                                        >  1:623741/1‑215 (MQ=255)                                                                                    ggTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACgggg                                                                                                                                                    >  1:77559/1‑251 (MQ=255)                                                                                       tgattgatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCtt                                                                                                                                                 <  1:220733/251‑1 (MQ=255)                                                                                        gattgatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTg                                                                                                                                                >  1:726159/1‑251 (MQ=255)                                                                                           tgatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGgtg                                                                                                                                             <  1:119385/251‑1 (MQ=255)                                                                                           tgatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGgtg                                                                                                                                             <  1:122959/251‑1 (MQ=255)                                                                                            gatGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGgtgt                                                                                                                                            >  1:831207/1‑251 (MQ=255)                                                                                             atGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTAt                                                                                                                                                                                         >  1:126022/1‑205 (MQ=255)                                                                                               gCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGtgtg                                                                                                                                           >  1:510709/1‑249 (MQ=255)                                                                                                ccGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTc                                                                                                                                        <  1:481424/251‑1 (MQ=255)                                                                                                ccGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTc                                                                                                                                        <  1:352143/251‑1 (MQ=255)                                                                                                ccGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTc                                                                                                                                        <  1:30637/251‑1 (MQ=255)                                                                                                ccGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTc                                                                                                                                        <  1:236539/251‑1 (MQ=255)                                                                                                    ggCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGAtt                                                                                                                                                                                                                                     <  1:668804/154‑1 (MQ=255)                                                                                                      cGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTgg                                                                                                                                   <  1:610632/250‑1 (MQ=255)                                                                                                          aCGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGt                                                                                                                              <  1:363458/251‑1 (MQ=255)                                                                                                                  aaCTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCa                                                                                                                      >  1:294892/1‑251 (MQ=255)                                                                                                                    cTCAATTCACGCACAAAATTGGTGGCGTTAAATTTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCAc                                                                                                                     <  1:725102/251‑1 (MQ=255)                                                                                                                      cAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTgg                                                                                                                  >  1:545886/1‑251 (MQ=255)                                                                                                                        aTTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCGACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGAt                                                                                                                <  1:90523/251‑1 (MQ=255)                                                                                                                             cGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACt                                                                                                           <  1:157504/251‑1 (MQ=255)                                                                                                                               cacaAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTg                                                                                                          <  1:818354/250‑1 (MQ=255)                                                                                                                               cacaAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGc                                                                                                         <  1:216034/251‑1 (MQ=255)                                                                                                                               cacaAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACATTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGTTTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTg                                                                                                          >  1:540930/1‑250 (MQ=255)                                                                                                                                   aaaTTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAAc                                                                                                      >  1:770135/1‑250 (MQ=255)                                                                                                                                             cGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                           <  1:547803/251‑1 (MQ=255)                                                                                                                                                              gAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGa                                                                              <  1:535/248‑1 (MQ=255)                                                                                                                                                                aCGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAAt                                                                         >  1:723780/1‑251 (MQ=255)                                                                                                                                                                   ttACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATaa                                                                       <  1:372162/250‑1 (MQ=255)                                                                                                                                                                     aCCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGt                                                                    <  1:787590/251‑1 (MQ=255)                                                                                                                                                                                     cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGATCTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGt                                                                                                                              <  1:195739/177‑1 (MQ=255)                                                                                                                                                                                     cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                           >  1:429753/1‑212 (MQ=255)                                                                                                                                                                                     cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                           <  1:469108/212‑1 (MQ=255)                                                                                                                                                                                     cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGt                                                                    <  1:74989/235‑1 (MQ=255)                                                                                                                                                                                     cGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAaacaac                                                         >  1:407582/1‑246 (MQ=255)                                                                                                                                                                                       gTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGgtg                                                                                                                                             >  1:748079/1‑160 (MQ=255)                                                                                                                                                                                                  gATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAAt                                                                         >  1:136599/1‑217 (MQ=255)                                                                                                                                                                                                        gcgcATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCtt                                 >  1:398168/1‑251 (MQ=255)                                                                                                                                                                                                           cATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTAt                               >  1:873576/1‑250 (MQ=255)                                                                                                                                                                                                               cGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                           >  1:289599/1‑186 (MQ=255)                                                                                                                                                                                                               cGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATAc                                                                                           <  1:697740/186‑1 (MQ=255)                                                                                                                                                                                                               cGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAg                                                                     <  1:85544/208‑1 (MQ=255)                                                                                                                                                                                                               cGTGCCAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGttt                          >  1:264778/1‑251 (MQ=255)                                                                                                                                                                                                                    cAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAg                                                                     >  1:631360/1‑203 (MQ=255)                                                                                                                                                                                                                    cAACAGTTCGGCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAg                     >  1:736972/1‑251 (MQ=255)                                                                                                                                                                                                                              gCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAg                                                                                                                               <  1:589015/135‑1 (MQ=255)                                                                                                                                                                                                                              gCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGc                                                                                                         >  1:357999/1‑157 (MQ=255)                                                                                                                                                                                                                              gCGTTGATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGc            >  1:645426/1‑250 (MQ=255)                                                                                                                                                                                                                                   gATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGc                    >  1:267485/1‑237 (MQ=255)                                                                                                                                                                                                                                   gATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGTCACGCCGAata      >  1:462256/1‑251 (MQ=255)                                                                                                                                                                                                                                   gATCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAata      >  1:271347/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAg                                                                                                                               >  1:455495/1‑129 (MQ=255)                                                                                                                                                                                                                                    aTCTTGCTTGACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAatat     >  1:10389/1‑251 (MQ=255)                                                                                                                                                                                                                                      cttgcttgACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTg   <  1:862062/251‑1 (MQ=255)                                                                                                                                                                                                                                       ttgcttgACGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCACCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTgc  <  1:764957/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   TCATCACGCCAATTTTGTGACCTGGCATGAAATTGCCGAAAAAAGCGGCGCTAAAATTCATGTTTTGCCCATTGGCGATGATTGGTTGATTGATGCCGAGGCGTTACGGGAAAAACTCAATTCACGCACAAAATTGGTGGCGTTAAA‑TTTTGCGTCGAACGTTACCGGCACGCAGCAAGCGGTAAAACAACTGATTCAGCGCATTCGTGCCAACAGTTCGGCGTTGATCTTGCTTGATGCCGCGCAGGCGATTAGCCACATAAAAATCGATTTGGCAGAACTCGACGCGGATTTTATCGCGTTTTCCGCCCATAAAATTTATGGTCCGACGGGGCTTGGTGTGCTCAGTGGGAAGTTATCCGCACTGGATTTACTGCAACCGTTGCAATACGGCGGCAAAATGATCAATAAAGTCAGTAAACAACAAATTACCTTTGCCGAATTGCCTTATCGTTTGGAAGCCGGCACGCCGAATATTGC  >  1/1811005‑1811484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑