Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,795,180	A→C	E185A (GAA→GCA)	unknown →	UPF0319 protein YccT precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,795,180	0	A	C	100.0%	177.4 / NA	59	E185A (GAA→GCA)	unknown	UPF0319 protein YccT precursor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/23);  total (36/23)

ATTTCAATGCCTTTAAAAAGGCCCCGGTGATCAGCGTAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGAAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCGTGCGCTACCTAAAAATAAACATATCACG  >  1/1794940‑1795408                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     aTTTCAATGCCTTTAAAAAGGCCCCGGTGATCAGAGTAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCaaa                                                                                                                                                                                                                            >  1:892246/1‑251 (MQ=255) aTTTCAATGCCTTTAAAAAGGCCCCGGTGATCAGAGTAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCaaa                                                                                                                                                                                                                            >  1:238297/1‑251 (MQ=255)  tttCAATGCCTTTAAAAAGGCCCCGGTGATCAGAGTAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAg                                                                                                                                                                                                                           >  1:742374/1‑251 (MQ=255)  tttCAATGCCTTTAAAAAGGCCCCGGTGATCAGAGTAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAg                                                                                                                                                                                                                           >  1:664633/1‑251 (MQ=255)   ttCAATGCCTTTAAAAAGGCCACGGTGATCAGAGTAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTGAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGt                                                                                                                                                                                                                          <  1:875012/251‑1 (MQ=255)                     gCCCCGGTGATCAGAGTAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGaa                                                                                                                                                                                                        >  1:431388/1‑251 (MQ=255)                        ccGGTGATCAGAGTAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGt                                                                                                                                                                                                     <  1:256050/251‑1 (MQ=255)                                     tAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATg                                                                                                                                                                                        <  1:16054/251‑1 (MQ=255)                                              cTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATAc                                                                                                                                                                               <  1:69838/251‑1 (MQ=255)                                              cTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATAc                                                                                                                                                                               <  1:219869/251‑1 (MQ=255)                                              cTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATAc                                                                                                                                                                               <  1:268517/251‑1 (MQ=255)                                                    gcgcGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTa                                                                                                                                                                          <  1:106636/250‑1 (MQ=255)                                                           cATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAg                                                                                                                                                                  >  1:467411/1‑251 (MQ=255)                                                                 aaCCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTc                                                                                                                                                                                                                         >  1:892958/1‑190 (MQ=255)                                                                   cccAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACaaa                                                                                                                                                          >  1:306228/1‑251 (MQ=255)                                                                    ccAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAg                                                                                                                                                         <  1:802974/251‑1 (MQ=255)                                                                              tACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAg                                                                                                                                                                                                               >  1:643655/1‑187 (MQ=255)                                                                               aCTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGaa                                                                                                                                                                                                        >  1:790848/1‑193 (MQ=255)                                                                               aCTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGCGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAAcc                                                                                                                                              <  1:600669/251‑1 (MQ=255)                                                                                cTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTa                                                                                                                                                                          >  1:683508/1‑222 (MQ=255)                                                                                       cAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCAt                                                                                                                                      >  1:886650/1‑251 (MQ=255)                                                                                             ggTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTg                                                                                                                                >  1:265085/1‑251 (MQ=255)                                                                                              gTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATAc                                                                                                                                                                               >  1:723709/1‑203 (MQ=255)                                                                                              gTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATAc                                                                                                                                                                               <  1:363408/203‑1 (MQ=255)                                                                                                 tcttcCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTg                                                                                                                            >  1:684362/1‑251 (MQ=255)                                                                                                          ccGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAAc                                                                                                                     >  1:560484/1‑249 (MQ=255)                                                                                                          ccGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACaa                                                                                                                   >  1:477313/1‑251 (MQ=255)                                                                                                          ccGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACaa                                                                                                                   >  1:519223/1‑251 (MQ=255)                                                                                                                    gATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGc                                                                                                                                                                                                  >  1:820542/1‑162 (MQ=255)                                                                                                                    gATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATAc                                                                                                                                                                               >  1:725596/1‑181 (MQ=255)                                                                                                                    gATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGcc                                                                                                                                                                >  1:788453/1‑196 (MQ=255)                                                                                                                    gATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAaatt                                                                                                         >  1:723685/1‑251 (MQ=255)                                                                                                                    gATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAaatt                                                                                                         >  1:339197/1‑251 (MQ=255)                                                                                                                     aTTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAaatta                                                                                                        >  1:794211/1‑251 (MQ=255)                                                                                                                     aTTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAaatt                                                                                                         <  1:874807/250‑1 (MQ=255)                                                                                                                              ttAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGAt                                                                                               <  1:259877/251‑1 (MQ=255)                                                                                                                                      aTACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTg                                                                                                                                                                             <  1:113742/165‑1 (MQ=255)                                                                                                                                        aCAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCGCCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGaaa                                                                                     <  1:629193/251‑1 (MQ=255)                                                                                                                                          aaCGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAg                                                                                   <  1:298861/251‑1 (MQ=255)                                                                                                                                          aaCGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAg                                                                                   <  1:718985/251‑1 (MQ=255)                                                                                                                                                           cgccgccgTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCa                                                                    >  1:429407/1‑249 (MQ=255)                                                                                                                                                                       tgcctTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAgg                                                      >  1:629437/1‑251 (MQ=255)                                                                                                                                                                            ttGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAAt                                                 >  1:665592/1‑251 (MQ=255)                                                                                                                                                                                 aCCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATg                                            <  1:17498/251‑1 (MQ=255)                                                                                                                                                                                   cGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTc                                                                     <  1:70160/224‑1 (MQ=255)                                                                                                                                                                                     cTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTcc                                                                          >  1:791139/1‑217 (MQ=255)                                                                                                                                                                                           cTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACtt                                  >  1:707534/1‑251 (MQ=255)                                                                                                                                                                                           cTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACtt                                  >  1:213968/1‑251 (MQ=255)                                                                                                                                                                                           cTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACtt                                  >  1:644858/1‑251 (MQ=255)                                                                                                                                                                                               cACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCg                              <  1:805660/251‑1 (MQ=255)                                                                                                                                                                                                 cGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCGTg                            <  1:685941/251‑1 (MQ=255)                                                                                                                                                                                                     gACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGaaa                                                                                     <  1:79501/190‑1 (MQ=255)                                                                                                                                                                                                     gACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCg                              >  1:853259/1‑245 (MQ=255)                                                                                                                                                                                                          cTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCGTGCGCTACCTa                   >  1:865702/1‑251 (MQ=255)                                                                                                                                                                                                          cTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCGTGCGCTACCTa                   >  1:679494/1‑251 (MQ=255)                                                                                                                                                                                                             ccGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATAc                                                                                                                                                                               >  1:157906/1‑92 (MQ=255)                                                                                                                                                                                                                  gTAGCCGGTGGTGCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCGTGCGCTACCTAAAAATaaa           >  1:86623/1‑251 (MQ=255)                                                                                                                                                                                                                            gtgCGGCCACAGGTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCGTGCGCTACCTAAAAATAAACATATCACg  <  1:768986/250‑1 (MQ=255)                                                                                                                                                                                                                                       ggTAAAGTAGCAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCGTGCGCTACCTaaaaa               <  1:816203/226‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     ATTTCAATGCCTTTAAAAAGGCCCCGGTGATCAGCGTAAAAACCGCTTCCGGCGCGAACATACAAACCCAACAAGAATACTTAAAACAGGAAGGTTTCTTCCCAACCGTTAGCCTGATTAAAGACTTAGGCGAATACAACGCTTCCGGTGCAGCCGCCGCCGTGCCTGCCTTTGCAACCGCTTCAACTGCCACGCCGACCACTACCGTGGTAGCCGGTGGTGCGGCCACAGGTAAAGTAGAAAAAGGCAAAGTCACCTTACAAGGCGAGAATGTTGCCGAGCAAATGCTCCAATACTGGTATCAACAAGCCGACAAAGAAACGCAAACCCGTTTCATTAATTGGGTGAAAAAACAAAAATAAAATTAATTTTAGATACATTTGAAAAGTGAGCTTCCCGCTCACTTTTTGTTTAAGGAGAATTTATGCAAACCACTTATCGTGCGCTACCTAAAAATAAACATATCACG  >  1/1794940‑1795408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑