Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,782,017 T→C intergenic (‑276/‑24) unknown ← / → unknown Transcriptional activator MetR/5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine methyltransferase (EC 2.1.1.14)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,782,0170TC100.0% 98.9 / NA 34intergenic (‑276/‑24)unknown/unknownTranscriptional activator MetR/5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine methyltransferase (EC 2.1.1.14)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/13);  total (21/13)

CCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAATAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCTCCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATTATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGACTTGCAA  >  1/1781777‑1782259
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
ccGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAacac                                                                                                                                                                                                                                           <  1:290496/250‑1 (MQ=255)
    aTCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGATGTTTAGCCGTCTAAATCATTTAac                                                                                                                                                                                                                                             <  1:190254/244‑1 (MQ=255)
              aaaaCGACCCGTACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGa                                                                                                                                                                                                                                   >  1:401275/1‑244 (MQ=255)
              aaaaCGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTa                                                                                                                                                                                                                            <  1:218830/251‑1 (MQ=255)
                  cGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAacac                                                                                                                                                                                                                                           <  1:366302/232‑1 (MQ=255)
                                          gaagaaATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTc                                                                                                                                                                                                 >  1:142558/1‑250 (MQ=255)
                                                                                        cGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTAt                                                                                                                                                  <  1:539566/251‑1 (MQ=255)
                                                                                                       tttttATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAAt                                                                                                                                    >  1:350790/1‑250 (MQ=255)
                                                                                                        ttttATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCg                                                                                                                                  >  1:369229/1‑251 (MQ=255)
                                                                                                                                                         ttcATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCaa                                                                                    >  1:584881/1‑248 (MQ=255)
                                                                                                                                                                 cTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTAcgc                                                                                            >  1:396057/1‑232 (MQ=255)
                                                                                                                                                                            ttattaATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACAt                                                                                                            <  1:839882/205‑1 (MQ=255)
                                                                                                                                                                            ttattaATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCg                                                              >  1:67893/1‑251 (MQ=255)
                                                                                                                                                                            ttattaATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCg                                                              >  1:603875/1‑251 (MQ=255)
                                                                                                                                                                            ttattaATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCg                                                              >  1:601666/1‑251 (MQ=255)
                                                                                                                                                                                      gCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGa                                                                                                                              <  1:763996/177‑1 (MQ=255)
                                                                                                                                                                                      gCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGAtt                                                    >  1:213974/1‑251 (MQ=255)
                                                                                                                                                                                        ataataCCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTa                                                   >  1:146030/1‑250 (MQ=255)
                                                                                                                                                                                         taataCCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGaa                                                                                                                                                        <  1:83446/148‑1 (MQ=255)
                                                                                                                                                                                         taataCCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGAc                                                                                                              <  1:813943/190‑1 (MQ=255)
                                                                                                                                                                                           ataCCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCg                                                                    <  1:38645/230‑1 (MQ=255)
                                                                                                                                                                                                            aTTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAAc                                                                                                                                                                                                                     >  1:97629/1‑68 (MQ=255)
                                                                                                                                                                                                                  gCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTa                        >  1:801085/1‑251 (MQ=255)
                                                                                                                                                                                                                   cccACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTa                        >  1:684404/1‑250 (MQ=255)
                                                                                                                                                                                                                       cGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGcc                                                                                                  >  1:781403/1‑172 (MQ=255)
                                                                                                                                                                                                                       cGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGAt                    >  1:528669/1‑250 (MQ=255)
                                                                                                                                                                                                                       cGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGCATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGCGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGAt                    >  1:317004/1‑250 (MQ=255)
                                                                                                                                                                                                                        gTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATca                  >  1:837159/1‑251 (MQ=255)
                                                                                                                                                                                                                              aCGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGcc                                                                                                  >  1:470259/1‑165 (MQ=255)
                                                                                                                                                                                                                               cGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTa           >  1:261488/1‑251 (MQ=255)
                                                                                                                                                                                                                                 tttAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGa         <  1:292705/251‑1 (MQ=255)
                                                                                                                                                                                                                                  ttAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGAc        <  1:324646/251‑1 (MQ=255)
                                                                                                                                                                                                                                        gTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGAc                                                                                                              <  1:475937/143‑1 (MQ=255)
                                                                                                                                                                                                                                        gTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGACTTGCaa  >  1:437116/1‑251 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
CCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAATAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCTCCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATTATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGACTTGCAA  >  1/1781777‑1782259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑