Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,778,796	A→C	*632S (TAA→TCA)	unknown →	Probable zinc protease pqqL (EC 3.4.99.‑)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,778,796	0	A	C	100.0%	226.4 / NA	74	*632S (TAA→TCA)	unknown	Probable zinc protease pqqL (EC 3.4.99.‑)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (40/34);  total (40/34)

GGATTACGATCTGTACCAAACCAAGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTAAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACGGATTTCACTTATTTCATTATTGGTGATATTTCCCGATCCGAGGT  >  1/1778555‑1779042                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       ggATTACGATCTGTACCAAACCAAGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGAt                                                                                                                                                                                                                                                    >  1:36910/1‑246 (MQ=255)        gATCTGTACCAAACCAAGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCGAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACaa                                                                                                                                                                                                                                        >  1:422994/1‑251 (MQ=255)        gATCTGTACCAAACCAAGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACaa                                                                                                                                                                                                                                        >  1:644859/1‑251 (MQ=255)        gATCTGTACCAAACCAAGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACaa                                                                                                                                                                                                                                        >  1:807024/1‑251 (MQ=255)             gTACCAAACCAAGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGAt                                                                                                                                                                                                                                                    >  1:536440/1‑234 (MQ=255)                  aaaccaaGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATatcat                                                                                                                                                                                                                               >  1:274617/1‑250 (MQ=255)                     ccaaGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCggg                                                                                                                                                                                                                           >  1:756612/1‑251 (MQ=255)                        aGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGaaa                                                                                                                                                                                                                        >  1:208160/1‑251 (MQ=255)                              tCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAgg                                                                                                                                                                                                                  >  1:189271/1‑251 (MQ=255)                               cATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAAtg                                                                                                                                                                                                                                                <  1:407925/220‑1 (MQ=255)                                          aaCGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAAc                                                                                                                                                                                                      <  1:399646/251‑1 (MQ=255)                                              gCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGat                                                                                                                                                                                                                <  1:61784/237‑1 (MQ=255)                                              gCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGCTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAAt                                                                                                                                                                                                  >  1:89749/1‑251 (MQ=255)                                                    aCCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATg                                                                                                                                                                                             >  1:472649/1‑250 (MQ=255)                                                          gTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGaaa                                                                                                                                                                                       >  1:471921/1‑250 (MQ=255)                                                          gTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGaaa                                                                                                                                                                                       >  1:167527/1‑250 (MQ=255)                                                                  tACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTAttt                                                                                                                                                                                                           <  1:73677/222‑1 (MQ=255)                                                                  tACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTa                                                                                                                                                                              >  1:706718/1‑251 (MQ=255)                                                                       gCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCaa                                                                                                                                                                         >  1:500851/1‑251 (MQ=255)                                                                                aaCTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAAc                                                                                                                                                                                                      <  1:695231/213‑1 (MQ=255)                                                                                aaCTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGACACAAAGTTTATGCAATCTATTTc                                                                                                                                                                 >  1:791029/1‑250 (MQ=255)                                                                                aaCTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTc                                                                                                                                                                 >  1:14275/1‑250 (MQ=255)                                                                                  cTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTa                                                                                                                                                              <  1:467046/251‑1 (MQ=255)                                                                                   tAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTAt                                                                                                                                                             <  1:686690/251‑1 (MQ=255)                                                                                    aaCGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTAtt                                                                                                                                                            >  1:629798/1‑251 (MQ=255)                                                                                          ggcagATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAAtg                                                                                                                                                                                                                                                <  1:652533/161‑1 (MQ=255)                                                                                          ggcagATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGat                                                                                                                                                                                                                <  1:491824/193‑1 (MQ=255)                                                                                          ggcagATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTgcgc                                                                                                                                                       >  1:63543/1‑250 (MQ=255)                                                                                              gATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTAt                                                                                                                                                             <  1:264300/240‑1 (MQ=255)                                                                                               aTGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGAcc                                                                                                                                                  <  1:733874/250‑1 (MQ=255)                                                                                                 gTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTAt                                                                                                                                                             <  1:525794/237‑1 (MQ=255)                                                                                                 gTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTaa                                                                                                                                               >  1:638482/1‑251 (MQ=255)                                                                                                    aacaacCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAacac                                                                                                                                            <  1:659232/251‑1 (MQ=255)                                                                                                    aacaacCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAacac                                                                                                                                            <  1:219365/251‑1 (MQ=255)                                                                                                     acaacCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACt                                                                                                                                           <  1:730563/251‑1 (MQ=255)                                                                                                      caacCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAacac                                                                                                                                            >  1:851506/1‑249 (MQ=255)                                                                                                            ctTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTTCCGGTGTGGCAAAACCGCAAGATCTCAGGAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAg                                                                                                                                    <  1:181730/251‑1 (MQ=255)                                                                                                            ctTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAg                                                                                                                                    <  1:280722/251‑1 (MQ=255)                                                                                                                ggTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTta                                                                                                                                >  1:712471/1‑251 (MQ=255)                                                                                                                ggTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTta                                                                                                                                >  1:165045/1‑251 (MQ=255)                                                                                                                   cAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATAc                                                                                                                             <  1:847600/251‑1 (MQ=255)                                                                                                                            cTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTGCCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAacac                                                                                                                                            <  1:40870/227‑1 (MQ=255)                                                                                                                                tgtTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCcaaaatcaaaat                                                                                                                >  1:124963/1‑251 (MQ=255)                                                                                                                                    ggCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGaa                                                                                                             >  1:305253/1‑250 (MQ=255)                                                                                                                                        aCGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAacac                                                                                                                                            >  1:489042/1‑215 (MQ=255)                                                                                                                                         cGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACagctt                                                                                                       >  1:522069/1‑251 (MQ=255)                                                                                                                                           gTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGc                                                                                                                                                                                            >  1:186987/1‑164 (MQ=255)                                                                                                                                             tATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACagcttagct                                                                                                   <  1:386571/251‑1 (MQ=255)                                                                                                                                                   tgaCGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAg                                                                                             <  1:884636/251‑1 (MQ=255)                                                                                                                                                     aCGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGc                                                                                            <  1:152673/250‑1 (MQ=255)                                                                                                                                                     aCGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGc                                                                                            <  1:662063/250‑1 (MQ=255)                                                                                                                                                      cGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAg                                                                                             >  1:469543/1‑248 (MQ=255)                                                                                                                                                                ggTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCcaaaatcaaaat                                                                                                                <  1:362777/219‑1 (MQ=255)                                                                                                                                                                ggTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAg                                                                                >  1:234764/1‑251 (MQ=255)                                                                                                                                                                        cGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAAttt                                                                        <  1:737437/251‑1 (MQ=255)                                                                                                                                                                         ggTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTa                                                                       >  1:771609/1‑251 (MQ=255)                                                                                                                                                                          gtgtGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAACGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTAt                                                                      >  1:535758/1‑251 (MQ=255)                                                                                                                                                                             tgGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTa                                                                                  <  1:753035/236‑1 (MQ=255)                                                                                                                                                                             tgGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCa                                                                    <  1:385524/250‑1 (MQ=255)                                                                                                                                                                                     ccGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGAt                                                           >  1:167106/1‑251 (MQ=255)                                                                                                                                                                                             tctcAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAg                                                                                                                                    <  1:359439/170‑1 (MQ=255)                                                                                                                                                                                                  gtagtTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACg                                              >  1:150026/1‑251 (MQ=255)                                                                                                                                                                                                        tGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTa                                                                                  >  1:401493/1‑209 (MQ=255)                                                                                                                                                                                                            gACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGc                                                                                                                                                                           <  1:516332/116‑1 (MQ=255)                                                                                                                                                                                                                 ttttCCGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACGGATTTCACTTATTTc                               <  1:445129/251‑1 (MQ=255)                                                                                                                                                                                                                      cGTCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACGGATTTCACTTATTTCattat                          <  1:270572/251‑1 (MQ=255)                                                                                                                                                                                                                        tCTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCCGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACGGATTTCACTTATTTCATTATTg                        <  1:353312/251‑1 (MQ=255)                                                                                                                                                                                                                         cTGAAACTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACagcttagc                                                                                                    <  1:329642/174‑1 (MQ=255)                                                                                                                                                                                                                              aCTCCAATCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACagcttagc                                                                                                    >  1:64074/1‑169 (MQ=255)                                                                                                                                                                                                                                    aTCCGCTCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTa                                                                                                                                                              >  1:452896/1‑105 (MQ=255)                                                                                                                                                                                                                                          tCCCGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACGGATTTCACTTATTTCATTATTGGTGATATTTCCCGATCCg      <  1:559356/251‑1 (MQ=255)                                                                                                                                                                                                                                             cGTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACGGATTTCACTTATTTCATTATTGGTGATATTTCCCGATCCGAgg   >  1:683793/1‑251 (MQ=255)                                                                                                                                                                                                                                              gTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACGGATTTCACTTATTTCATTATTGGTGATATTTCCCGATCCGAGGt  >  1:520817/1‑251 (MQ=255)                                                                                                                                                                                                                                              gTTTCAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACGGATTTCACTTATTTCATTATTGGTGATATTTCCCGATCCGAGGt  >  1:809820/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       GGATTACGATCTGTACCAAACCAAGATTATCATTTATTGCGAACGGCGATTACCTTAGTTGATGATACCGGCACAGGTGAACTAACGCAGGCAGATGTCAACAACCTCTTTGGTCAAAGCCCACTTGTGTTGGCAACGGTTATTGATGACGATAAGCAAGGTTTTACCGGTGTGGCAAAACCGCAAGATCTCAGTAGTTTGCTGACACTTTTCCGTCTGAAACTCCAATCCGCTCCCGTTTAAGATAATGTGTTACAAAAATATCATCGGGAAACACAGGATTATTTTAAACAAATTGATGCCGAAACAAAGTTTATGCAATCTATTTCCTATTTGCGCCGACCTAACACTGCTACAGTTTATACCCAAAATCAAAATGAACAGCTTAGCTTTACAGCTGCACAATTAAGTCAAATTTATCAAGAAAAGATTTTAGGTAAAACGGATTTCACTTATTTCATTATTGGTGATATTTCCCGATCCGAGGT  >  1/1778555‑1779042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑