BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,761,227	A→G	P150P (CCT→CCC)	unknown ←	O‑succinylbenzoic acid‑‑CoA ligase (EC 6.2.1.26)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,761,227	0	A	G	100.0%	304.6 / NA	107	P150P (CCT→CCC)	unknown	O‑succinylbenzoic acid‑‑CoA ligase (EC 6.2.1.26)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (58/49); total (58/49)

Only 100 of 107 total aligned reads displayed.

GGTCACTTGATTCACTGCCGCATAAAAATCTTCACCGGGTAACACCAAGGTGGTAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCAGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAACACCCGCGCCCCCGTCTGAATCGCCGCCAA  >  1/1760981‑1761472

ggTCACTTGATTCACTGCCGCATAAAAATCTTCACCGGGTAACACCAAGGTGGTAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGt                                                                                                                                                                                                                                                     <  1:207290/250‑1 (MQ=255)

                     aTAAAAATCTTCACCGGGTAACACCAAGGTGGTAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACAt                                                                                                                                                                                                                               >  1:551597/1‑251 (MQ=255)

                          aaTCTTCACCGGGTAACACCAAGGTGGTAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAGCTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAg                                                                                                                                                                                                                          <  1:715782/251‑1 (MQ=255)

                                ccccGGGTAACACCAAGGTGGTAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCg                                                                                                                                                                                                                    <  1:835387/249‑1 (MQ=255)

                                         acacCAAGGTGGTAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCg                                                                                                                                                                                                                    <  1:733585/242‑1 (MQ=255)

                                          cacCAAGGTGGTAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAatttgat                                                                                                                                                                                                          >  1:480861/1‑251 (MQ=255)

                                                 gtggtAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGcc                                                                                                                                                                                                     <  1:413073/249‑1 (MQ=255)

                                                 gtggtAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGc                                                                                                                                                                                                      >  1:433686/1‑248 (MQ=255)

                                                  tggtAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGcc                                                                                                                                                                                                     >  1:219019/1‑248 (MQ=255)

                                                                     acGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAatttgatttg                                                                                                                                                                                                       >  1:48757/1‑227 (MQ=255)

                                                                          acacaATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTaaa                                                                                                                                                                         >  1:490291/1‑251 (MQ=255)

                                                                           cacaATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTaaaa                                                                                                                                                                        <  1:105368/251‑1 (MQ=255)

                                                                           cacaATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTaaaa                                                                                                                                                                        >  1:95193/1‑251 (MQ=255)

                                                                           cacaATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTaaaa                                                                                                                                                                        >  1:875413/1‑251 (MQ=255)

                                                                            acaATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTaaaaa                                                                                                                                                                       <  1:348516/251‑1 (MQ=255)

                                                                                 ccccTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAaca                                                                                                                                                                    >  1:316938/1‑249 (MQ=255)

                                                                                      gTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGcc                                                                                                                                                                                               <  1:531229/218‑1 (MQ=255)

                                                                                      gTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAg                                                                                                                                                                                >  1:348986/1‑232 (MQ=255)

                                                                                      gTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTaaaa                                                                                                                                                                        >  1:48823/1‑240 (MQ=255)

                                                                                      gTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGaaa                                                                                                                                                             >  1:151961/1‑251 (MQ=255)

                                                                                      gTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTACACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGaaa                                                                                                                                                             >  1:654957/1‑251 (MQ=255)

                                                                                           gagaCATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGt                                                                                                                                                                             >  1:360826/1‑230 (MQ=255)

                                                                                               cATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCg                                                                                                                                                    >  1:12714/1‑251 (MQ=255)

                                                                                                 tGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACa                                                                                                                                                                                                                                <  1:39450/174‑1 (MQ=255)

                                                                                                 tGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAac                                                                                                                                                                     <  1:764283/232‑1 (MQ=255)

                                                                                                 tGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCa                                                                                                                                                  >  1:506749/1‑251 (MQ=255)

                                                                                                  ggTATAAGGGCAAGAAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCTGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAg                                                                                                                                                                                                                      <  1:285122/183‑1 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATc                                                                                                                                                                                                                              <  1:198469/174‑1 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAAt                                                                                                                                                           >  1:309626/1‑240 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGAc                                                                                                                                                      >  1:642163/1‑245 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCa                                                                                                                                                  >  1:259479/1‑249 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCa                                                                                                                                                  >  1:492140/1‑249 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCa                                                                                                                                                  >  1:206488/1‑249 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCa                                                                                                                                                  >  1:392824/1‑249 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCa                                                                                                                                                  >  1:631218/1‑249 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCa                                                                                                                                                  >  1:76037/1‑249 (MQ=255)

                                                                                                   gTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGCGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTTCCTGTGCCATTGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCa                                                                                                                                                  >  1:64601/1‑249 (MQ=255)

                                                                                                                       gcCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCaaaa                                                                                                                            >  1:581712/1‑250 (MQ=255)

                                                                                                                       gcCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAAt                                                                                                                            >  1:428463/1‑251 (MQ=255)

                                                                                                                           cGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGc                                                                                                                          >  1:66934/1‑249 (MQ=255)

                                                                                                                                ccGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAACGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTtctgc                                                                                                                   <  1:738247/251‑1 (MQ=255)

                                                                                                                                   cTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTtctgctc                                                                                                                 <  1:698432/250‑1 (MQ=255)

                                                                                                                                   cTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTtctgctc                                                                                                                 <  1:13755/250‑1 (MQ=255)

                                                                                                                                   cTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTtctgctc                                                                                                                 <  1:283315/250‑1 (MQ=255)

                                                                                                                                     gCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTtctgctctg                                                                                                               >  1:422691/1‑250 (MQ=255)

                                                                                                                                     gCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTtctgctctg                                                                                                               >  1:72221/1‑250 (MQ=255)

                                                                                                                                      cGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGACTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTtctgctctgc                                                                                                              <  1:191381/250‑1 (MQ=255)

                                                                                                                                          aaaGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCgtaa                                                                                                         <  1:855148/251‑1 (MQ=255)

                                                                                                                                          aaaGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCgtaa                                                                                                         <  1:600188/251‑1 (MQ=255)

                                                                                                                                             gTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGc                                                                                                                                                                                                <  1:249799/162‑1 (MQ=255)

                                                                                                                                             gTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCgtaaagt                                                                                                      >  1:6710/1‑251 (MQ=255)

                                                                                                                                             gTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCgtaaagt                                                                                                      >  1:777946/1‑251 (MQ=255)

                                                                                                                                              ttCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCgtaaag                                                                                                       <  1:829542/249‑1 (MQ=255)

                                                                                                                                                cATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGt                                                                                                                                                                             >  1:221841/1‑177 (MQ=255)

                                                                                                                                                  ttAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTaaaaa                                                                                                 <  1:203987/251‑1 (MQ=255)

                                                                                                                                                    aaCTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATc                                                                                               <  1:834751/251‑1 (MQ=255)

                                                                                                                                                        cacacaCGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGAc                                                                                                                                                      <  1:899886/192‑1 (MQ=255)

                                                                                                                                                          cacacGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGt                                                                                                                                                                                                                                                     >  1:879118/1‑96 (MQ=255)

                                                                                                                                                              cGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTa                                                                                     >  1:554082/1‑251 (MQ=255)

                                                                                                                                                                 cTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGAt                                                                                  <  1:877399/251‑1 (MQ=255)

                                                                                                                                                                      gCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCGTTTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGa                                                                               >  1:609539/1‑250 (MQ=255)

                                                                                                                                                                       cGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACaa                                                                            <  1:39663/251‑1 (MQ=255)

                                                                                                                                                                       cGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACaa                                                                            <  1:322333/251‑1 (MQ=255)

                                                                                                                                                                       cGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCAGTAAGCCTGTGGACCCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGGGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAATAAAAATCCGCATGATTAGATTGACaa                                                                            <  1:204347/251‑1 (MQ=255)

                                                                                                                                                                           aTCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGAtt                                                                                      >  1:885620/1‑237 (MQ=255)

                                                                                                                                                                           aTCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACaa                                                                            <  1:462913/247‑1 (MQ=255)

                                                                                                                                                                           aTCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAagag                                                                        >  1:530036/1‑251 (MQ=255)

                                                                                                                                                                            tCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGc                                                                       <  1:899644/251‑1 (MQ=255)

                                                                                                                                                                            tCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGc                                                                       <  1:125686/251‑1 (MQ=255)

                                                                                                                                                                                    ggCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAatttgat                                                                                                                                                                                                          >  1:746245/1‑113 (MQ=255)

                                                                                                                                                                                        tGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGCTAAAAAACACTTGAAAATTTGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTTCTCTGCCGAAAAGTAAAAATCCGCATGATTAGATTGACAAAGATCAATGCGttttt                                                            >  1:710905/1‑250 (MQ=255)

                                                                                                                                                                                         gTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACaa                                                                            <  1:751965/233‑1 (MQ=255)

                                                                                                                                                                                          tACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTcc                                                          <  1:376111/250‑1 (MQ=255)

                                                                                                                                                                                           aCATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTACTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCaa                                                        >  1:451392/1‑251 (MQ=255)

                                                                                                                                                                                             aTTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTttgttg                                                                                                                                         >  1:787809/1‑168 (MQ=255)

                                                                                                                                                                                             aTTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACg                                                      >  1:742957/1‑251 (MQ=255)

                                                                                                                                                                                                  gCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTtctgc                                                                                                                   <  1:761310/185‑1 (MQ=255)

                                                                                                                                                                                                    aCTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACAt                                               <  1:308447/251‑1 (MQ=255)

                                                                                                                                                                                                     cTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACAt                                               >  1:557287/1‑250 (MQ=255)

                                                                                                                                                                                                     cTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCACGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATc                                              <  1:503936/251‑1 (MQ=255)

                                                                                                                                                                                                         ggCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGcgt                                                                                                                                                                                        <  1:254101/110‑1 (MQ=255)

                                                                                                                                                                                                         ggCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACaa                                                                            >  1:666371/1‑217 (MQ=255)

                                                                                                                                                                                                          gCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGAtt                                         >  1:483673/1‑251 (MQ=255)

                                                                                                                                                                                                              tCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGc                                     <  1:283652/251‑1 (MQ=255)

                                                                                                                                                                                                               cGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGc                                     <  1:8018/250‑1 (MQ=255)

                                                                                                                                                                                                               cGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCa                                    <  1:119582/251‑1 (MQ=255)

                                                                                                                                                                                                                     gCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTtctgctctgc                                                                                                              <  1:560714/171‑1 (MQ=255)

                                                                                                                                                                                                                     gCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAaca                              >  1:219215/1‑251 (MQ=255)

                                                                                                                                                                                                                      ccTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAacac                             <  1:840392/251‑1 (MQ=255)

                                                                                                                                                                                                                      ccTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAacac                             <  1:403081/251‑1 (MQ=255)

                                                                                                                                                                                                                      ccTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATCAAGCATTAacac                             <  1:59656/251‑1 (MQ=255)

                                                                                                                                                                                                                       cTGTGGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAACAcc                            <  1:175036/251‑1 (MQ=255)

                                                                                                                                                                                                                         gtgGAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACAt                                               <  1:101778/230‑1 (MQ=255)

                                                                                                                                                                                                                            gAACCGGAAGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAACACCcgcg                        >  1:663276/1‑250 (MQ=255)

                                                                                                                                                                                                                                    aGTGAGCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCGGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAACACCCGCGcccc                    >  1:411314/1‑246 (MQ=255)

                                                                                                                                                                                                                                         gCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGAtt                                         >  1:122134/1‑220 (MQ=255)

                                                                                                                                                                                                                                         gCGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAACACCCGCGCCCCCGTCTGAAt           >  1:609743/1‑250 (MQ=255)

                                                                                                                                                                                                                                          cGTCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAACACCCGCGCCCCCGTCTGAAt           <  1:726788/249‑1 (MQ=255)

                                                                                                                                                                                                                                            tCATGGTGGCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAACACCCGCGCCCCCGTCTGAATcgc        >  1:732189/1‑250 (MQ=255)

                                                                                                                                                                                                                                                 gtggCGGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAACACCCGCGCCCCCGTCTGAATCGCCGCCaa  >  1:324810/1‑251 (MQ=255)

GGTCACTTGATTCACTGCCGCATAAAAATCTTCACCGGGTAACACCAAGGTGGTAGCGCCGGTCAACCAACGCCACACAATCCCCTGTCCGGAGACATGGTATAAGGGCAAGGAAAGCAGCCACGAATCCGCTGCGTCAAAGTTCATTAACTCACACACGCCTCGGGCGTTATCCAAATGGGCTTGTACATTGTGCACTACGGCTTTCGGTAAGCCTGTGGAACCGGAAGTGAGCGTCATGGTGGCAGGTTGAGAAAAATCAATATCAACATCCGAGTAAGCGTGAATTTGATTTGCCTGTGCCATGGCGTCG‑TTCAGGGTTAAAAAACACTTGAAAATTGGACCGCACTTTTGTTGCTCGCTAGCAAATGCCTTCTGCTCTGCCGTAAAGTAAAAATCCGCATGATTAGATTGACAAAGAGCAATGCGTTTTTCCAACGGGAACATCGGATTAAGCATTAACACCCGCGCCCCCGTCTGAATCGCCGCCAA  >  1/1760981‑1761472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`