| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,722,985 | T→G | I81L (ATT→CTT) | unknown ← | N‑acetylmannosamine‑6‑phosphate 2‑epimerase (EC 5.1.3.9) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,722,985 | 0 | T | G | 97.4% | 202.4 / ‑5.3 | 79 | I81L (ATT→CTT) | unknown | N‑acetylmannosamine‑6‑phosphate 2‑epimerase (EC 5.1.3.9) |
| Reads supporting (aligned to +/- strand): ref base T (0/2); major base G (38/37); minor base A (0/2); total (38/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GGTATAGCCCGACATGGTGCTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AATATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAG > 1/1722740‑1723227 | ggTATAGCCCGACATGGTGCTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATcc > 1:884738/1‑250 (MQ=255) gTATAGCCCGACATGGTGCTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCg > 1:126876/1‑251 (MQ=255) aCATGGTGCTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATa > 1:683218/1‑251 (MQ=255) aCATGGTGCTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATa > 1:709122/1‑251 (MQ=255) aCATGGTGCTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATa > 1:315558/1‑251 (MQ=255) aCATGGTGCTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATa > 1:1941/1‑251 (MQ=255) gTGCTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCgat > 1:769065/1‑251 (MQ=255) cTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCgatgat > 1:835281/1‑251 (MQ=255) cGATGTCAAAACCGAGTTGTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGcac < 1:631814/250‑1 (MQ=255) tCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTa < 1:476011/250‑1 (MQ=255) tCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTa < 1:390215/250‑1 (MQ=255) aaaaCCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACgg < 1:835825/251‑1 (MQ=255) aaaaCCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTCTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACgg < 1:438625/251‑1 (MQ=255) cGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCggg < 1:639256/251‑1 (MQ=255) tGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCgatg > 1:314725/1‑222 (MQ=255) aTACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACTGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTa > 1:205246/1‑251 (MQ=255) acaacttttcgtcCAGATTGGTGCTATCCGCCATGGCATGACAGCCCGTTTCATGAATTTTTTTAATCGCACTTTCACTCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGGTACGTACCGTGCTGTCGGG‑AAGATCCCGTTTGACGATACCTCTGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTaaa < 1:227796/240‑1 (MQ=255) cAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑t > 1:622373/1‑251 (MQ=255) cAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑t < 1:55578/251‑1 (MQ=255) cccTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCgatga < 1:758966/212‑1 (MQ=255) cccTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGt > 1:483763/1‑251 (MQ=255) cccTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGt > 1:548099/1‑251 (MQ=255) tcCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACAcc < 1:12908/251‑1 (MQ=255) gATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑t > 1:554910/1‑237 (MQ=255) aTTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTc < 1:744422/249‑1 (MQ=255) aTTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCaa < 1:159566/251‑1 (MQ=255) tgaaGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑Tg < 1:463956/248‑1 (MQ=255) cAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCa < 1:657603/250‑1 (MQ=255) tGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACgg > 1:422037/1‑198 (MQ=255) tGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑Tg < 1:121438/236‑1 (MQ=255) cATGAATTTTTTTACCCGCACTTTCAATCTCTCCCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGAAAAAACGGGGTGATACGTCCCGGGCTGTCGGA‑AAGACCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CCCGGTTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGcc < 1:467550/251‑1 (MQ=255) cATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAAccccgccgccc < 1:849542/234‑1 (MQ=255) acccgcctttccAATCTCTCCGGGGGGGGGGCGAGGAGGGCCACCCAGGGCGATAATGCCCGCTCGGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAACGGGGGTGATCCGTCCGGGGCGGTGGGG‑AAGACCCCGTTTGACGATACCGATGATCGGCACATTTCCGGTGGGG‑CG‑CAGGGTTTTTAAT‑TTGCCCCCACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATagcag < 1:724703/240‑1 (MQ=255) cACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACa > 1:214982/1‑251 (MQ=255) tttccactctcTACCGGGGGGGGGAGACGAGGCCCATCCACGGCGATAATGTCCGCCCGGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTGGGG‑AAAATCCCGTTTGACAATACCGATGATCGGCACTTTTACGGTGGGG‑CG‑CGCGG‑TTTTAAATTTGCCCACACCTCCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAg < 1:606526/222‑1 (MQ=255) cTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑t < 1:229624/181‑1 (MQ=255) ttCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAAttt > 1:632137/1‑251 (MQ=255) tctcTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGt > 1:650004/1‑251 (MQ=255) ctACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTa > 1:168125/1‑251 (MQ=255) gggcgACGAGCGCCACCCAGGGCGATATTGCCCGCTCCGGCGTGCGCCAATTCTTCAATG‑TCCTGAAAAACCGGGGTGATCCGTCCGGGGCTGGCGGG‑AAGACCCCTTTGGCCGAAACCGATGATCGGCACATT‑‑CGGGGGGG‑GGTCGCGGTTTTAAAA‑TTGCCCACCCCTTCAA‑GGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGAcc < 1:220468/250‑1 (MQ=255) ggcgACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTaaa < 1:48836/158‑1 (MQ=255) gacgaGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGt > 1:428009/1‑136 (MQ=255) acgaGTGCCACCCACGGCGATATTGTCGGCCCCGGCGGGCGCAAAGTCTTCAATG‑TCCTGAAAAAAGGGGGTGATGCGTCCCGGGCTGTGGGA‑AAGACCCCTTTTGACGATCCCGATGATCGGCACTTTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGCCCACACCTTCAA‑GGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACcatcat < 1:584297/251‑1 (MQ=255) cACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGa > 1:688812/1‑250 (MQ=255) gCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAaga > 1:508371/1‑251 (MQ=255) gCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAaga > 1:109771/1‑251 (MQ=255) gCGATAATGCCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑CCCGAAAAAAACGGGGTGATACGTACCGGGCTGTGGGG‑AAGACCCCGTTTGACGATACCGATGATCGGCACATTGACGGTCGGG‑CG‑CACGGTTTTTAAA‑TTGCCCCCCCCTCCAA‑TGCGCAACCCCCCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAag < 1:152315/250‑1 (MQ=255) gATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAgg > 1:576867/1‑251 (MQ=255) aTAATGCCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGAAAAAACGGGGTGATACGTCCGGGGCTGTCGGG‑AAGACCCCTTTTGACGATACCGATGATCGCCACATTTACGGTCGGG‑CG‑CACGTCTTTTAAA‑TTGTCCACCCCTTCAA‑GGCGCACCCCCCCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAgg < 1:548378/250‑1 (MQ=255) acgggcccgcccgggCGTGCCCAAATTCTTCAATT‑CCCTGAAAAAGCGGGGTGATGCGTACCGGGCTGTCGGA‑AAGACCCCTTTTGACAACCCCGATGTTCGGCACATTTACGGCCGGG‑CC‑CACGGTTTTTAAT‑TTGCCCACCCCTACAA‑TGCCCACCCCCGCCCCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAgg < 1:150409/236‑1 (MQ=255) ccGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑t < 1:487663/129‑1 (MQ=255) ggcgggcgCCAAGTCTTAATTG‑TCCTGAAAAAACGGGGGGATACGCACCGGGCTG‑CGGGAAAGATCCCGTTTGCCGATACGGAGGATCGCCACATTTCCGGTCGGG‑CG‑CACGGTTTTTAAA‑TTGTCCCCCCCTCAAA‑TGCGCACCCCCCCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTc < 1:257526/250‑1 (MQ=255) ccgcCAAGTC‑TCAATGTTCATGAAAAAGCGGGTTGATACATCCGGGGCTGGCGGA‑AAAACCCCTTTGGAGGATACCGATGA‑CGGCAATTTTACCGTCGGGCCG‑CA‑GCCTTTTTAA‑TTTCCCCCCCCTTAAATTGCGC‑ACCCCGCCCCCCCTGCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAg < 1:113418/249‑1 (MQ=255) aGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCg > 1:539883/1‑251 (MQ=255) gTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCaa > 1:112899/1‑125 (MQ=255) gTCTTCAATG‑TCCGGTAAAAACGGGGTGATCCGTCCCGGGCTGTCGGG‑AAGATCCCGTTTGACGATCCCGATGATCGGCACTTTTCCGGTCAGG‑CG‑CACGGTTTTAAAA‑TTGCCCACCCCTACAA‑GGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCgg < 1:359044/186‑1 (MQ=255) gTCTTAAATG‑TCCTGAAAAAGGGGGGTGATACGTACGGGGCTGTCGGG‑AAGATCCCGTTTGACGATCCCGATGATCGGCACATTTACGGGGGGG‑CG‑CACGGTTTTTAAA‑TTTTCCCCCCCTTCAA‑GGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCAc < 1:277529/210‑1 (MQ=255) cTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTaaa > 1:895932/1‑107 (MQ=255) tCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGaaaa > 1:451254/1‑250 (MQ=255) g‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCtt > 1:658673/1‑251 (MQ=255) cTGTAAAAGCGGGGTGATACGTCCGGGGCTGTGGGG‑AAGATCCCGTTTGACGATACGGATGATCGGCACATTTACGGGCGGG‑CG‑CGCGGTTTTTAAA‑TTGTCCACACCTTCAG‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTtgat < 1:91346/251‑1 (MQ=255) gTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTtgatga > 1:239835/1‑251 (MQ=255) aaacgggggTGATCCTTCCCGGGCTGTGGGG‑AATACCCCTTTTGCCGATACCAATGATCGCCACATTTCCGGTCGGG‑CG‑CCCGGCTTTTAAT‑TTGCCCACCCCTTCAA‑TGCGCACCCCCGCCCCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCAc < 1:675361/188‑1 (MQ=255) gTGATCCGTCCCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCatcatc < 1:331252/182‑1 (MQ=255) gATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATCCCGATGATCGGCACATTTCCGGTCGGG‑CG‑CACGGTTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAAc < 1:21594/228‑1 (MQ=255) gATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTgag > 1:474853/1‑251 (MQ=255) gATACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTgag > 1:18091/1‑251 (MQ=255) aaTACGTACGGGTCTGTCGGG‑AATATCCCGTTTGACATTACCGATGATCGGAACATTCGCGGTCGGG‑CG‑CATGGTTTTTAAT‑TTGTCCCCACTTACAT‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTgag < 1:35786/250‑1 (MQ=255) atccGTACCGGGCTGTCGGA‑AAGTCCCCTTTTGACGATCCCGATGACCGCCACATTTCCGGTCGGG‑C‑‑CCCCGCTTTTAAATTTGCCCCCCCCTCCAA‑TCCCCACCCCCCCCCCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTgag < 1:897889/247‑1 (MQ=255) aTACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACgg > 1:565754/1‑61 (MQ=255) aTACGTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATagcag > 1:826715/1‑145 (MQ=255) gtcccGGGCTGTCGGG‑AAGACCCCGTTTGACGATCCCGATGATCGGCACATTTACGGTCGGG‑CG‑CCCGGTTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGc < 1:638053/179‑1 (MQ=255) gTACGGGGCTGTGGGG‑AAGTCCCCGTTTGACGATCCCGATGATCGGCACATTTACGGCCGGG‑CG‑CACGGTTTTTAAT‑TTGTCCACCCCTTAAA‑GGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTaa < 1:492870/199‑1 (MQ=255) gTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTGAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAg > 1:271564/1‑250 (MQ=255) gTACCGGGCTGTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAg > 1:413553/1‑250 (MQ=255) ccggggCTGTGGGG‑AAGACCCCTTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGTTTTTAAA‑TTGTCCACCCCTTCAA‑TGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCtg < 1:695615/248‑1 (MQ=255) gTCGGG‑AAGATCCCGTTTGACGATCCCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGTTTTAAAA‑TTGTCCACCCCTTCAA‑GGCGCACCCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAg < 1:690844/201‑1 (MQ=255) gTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACa > 1:141668/1‑248 (MQ=255) gTCGGG‑AAGATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAg > 1:383009/1‑251 (MQ=255) | GGTATAGCCCGACATGGTGCTACCCACGATGTCAAAACCGAGTTTTTGGCAATACAACCCTTCTTCCAGATTGGAGCAATCCGCCATGGCAAGACAGCCCATTTCATGAATTTTTTTAACCGCACTTTCAATCTCTACCGGGCGCGGGCGACGAGTGCCATCCACGGCGATAATGTCCGCTCCGGCGTGCGCCAAGTCTTCAATG‑TCCTGTAAAAACGGGGTGATACGTACCGGGCTGTCGGG‑AATATCCCGTTTGACGATACCGATGATCGGCACATTTACGGTCGGG‑CG‑CACGGCTTTTAAA‑TTGTCCACACCTTCAA‑TGCGCAACCCCGCCGCCCC‑GCCGATAACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAG > 1/1722740‑1723227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |