Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,704,601	T→C	T184A (ACA→GCA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,704,601	0	T	C	100.0%	36.1 / NA	14	T184A (ACA→GCA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/9);  total (5/9)

ATTCTAATATTTCTATTCCATCGTCTTAAATAATCAACATTAAACTTAAAATATTTTAAACTTAACTTTTTGGCATACTCAAATAAACCATTATCATTAAAATATATTTTCAAAGATGCAAATACTCGTTCATACTCCATATATATAGATAAAAAATACAAAAAAGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGTATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTGAGTATTATATAAATAAAACAACTTATCTTTTTATTGTTTTCATTTAATGAATTGCATTTGATATATAAAAATAAAAACGTTGAAAAAGGTATCATAATAAACTCTATTAAAAAAGAAAAAGAATAAAGTTCAAGAA  >  1/1704369‑1704798                                                                                                                                                                                                                                         |                                                                                                                                                                                                      aTTCTAATATTTCTATTCCATCGTCTTAAATAATCAACATTAAACTTAAAATATTTTAAACTTAACTTTTTGGCATACTCAAATAAACCATTATCATTAAAATATATTTTCAAAGATGCAAATACTCGTTCATACTCCATATATATAGATAAAAAATACAAAAAAGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAAc                                                                                                                                                                                        <  1:290371/248‑1 (MQ=255)            tCTATTCCATCGTCTTAAATAATCAACATTAAACTTAAAATATTTTAAACTTAACTTTTTGGCATACTCAAATAAACCATTATCATTAAAATATATTTTCAAAGATGCAAATACTCGTTCATACTCCATATATATAGATAAAAAATACAAAAAAGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTg                                                                                                                                                                          <  1:126078/251‑1 (MQ=255)                                                                               tCAAATAAACCATTATCATTAAAATATATTTTCAAAGATGCAAATACTCGTTCATACTCCATATATATAGATAAAAAATACAAAAAAGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTgc                                                                                                                                         <  1:750906/217‑2 (MQ=255)                                                                                                                                 ttCATACTCCATATATATAGATAAAAAATACAAAAAAGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATaaa                                                                                                                                                                                 <  1:511880/127‑1 (MQ=255)                                                                                                                                 ttCATACTCCATATATATAGATAAAAAATACAAAAAAGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATcac                                                                                                                                                       <  1:71629/153‑1 (MQ=255)                                                                                                                                  tCATACTCCATATATATAGATAAAAAATACAAAAAAGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTGAGTATTATATAAATAAAACAACTTATCTTTTTATTGTTTTCATTTAATGAATTGCATTTGATATATAAAAATAAAAACGTTGaaaa                                                    <  1:56892/251‑1 (MQ=255)                                                                                                                                                             tACAAAAAAGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTGAGTATTATATAAATAAAACAACTTATCTTTTTATTGTTTTCATTTAATGAATTGCATTTGATATATAAAAATAAAAACGTTGAAAAAGGTATCATAATAAACTCTATTaaaaa                         <  1:189244/251‑1 (MQ=255)                                                                                                                                                                    aaGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTGAGTATTATataaata                                                                                                                           >  1:725756/1‑146 (MQ=255)                                                                                                                                                                      ggAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAAc                                                                                                                                                                                        >  1:258007/1‑83 (MQ=255)                                                                                                                                                                           aCATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTGAGTATTATATAAATAAAACAACTTATCTTTTTATTGTTTTCATTTAATGAATTGCATTTGat                                                                            >  1:461366/1‑186 (MQ=255)                                                                                                                                                                                    agaaagGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTGAGTATTATATAAATAAAACAACTTATCTTTTTATTGTTTTCATTTAATGAATTGCATTTGATATATAAAAATAAAAACGTTGAAAAAGGTATCATAATAAACTCTATTAAAAAAGAAAAAGAATAAAGTTCAAGaa  >  1:50188/1‑251 (MQ=255)                                                                                                                                                                                         ggATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTGAGTATTATATAAATAAaacaac                                                                                                                    <  1:370197/132‑1 (MQ=255)                                                                                                                                                                                                  gCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTGAGTATTATATAAATAAAACAACTTATCTTTTTATTGTTTTCATTTAATGAATTGCATTTGATATATAAAAATAAAAACGTTGAAAAAGGTATCataa                                         <  1:821165/198‑1 (MQ=255)                                                                                                                                                                                                                          cccATAAAGAATTTGCATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACt                                                                                                                                           >  1:477499/1‑76 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                      ATTCTAATATTTCTATTCCATCGTCTTAAATAATCAACATTAAACTTAAAATATTTTAAACTTAACTTTTTGGCATACTCAAATAAACCATTATCATTAAAATATATTTTCAAAGATGCAAATACTCGTTCATACTCCATATATATAGATAAAAAATACAAAAAAGGAATACATATTAAAGAAAGGATAGATGGCAAAAACAACTCTAGAATGTTGCCCCATAAAGAATTTGTATCAAATAACAAAACAGATAAATATATTGAATGTAAAAAATATATCACAATCCATAAACTGAGTATTATATAAATAAAACAACTTATCTTTTTATTGTTTTCATTTAATGAATTGCATTTGATATATAAAAATAAAAACGTTGAAAAAGGTATCATAATAAACTCTATTAAAAAAGAAAAAGAATAAAGTTCAAGAA  >  1/1704369‑1704798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑