Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,642,495	A→G	V361V (GTA→GTG)	unknown →	D‑3‑phosphoglycerate dehydrogenase (EC 1.1.1.95)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,642,495	0	A	G	100.0%	201.8 / NA	70	V361V (GTA→GTG)	unknown	D‑3‑phosphoglycerate dehydrogenase (EC 1.1.1.95)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/47);  total (23/47)

CAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTAGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAATATTAGTCATGATAAAAAGTGCGGTGGAAATTCATTGTGTTTTTCCACCGCACTTTTTTAGTGCGCCTAATC  >  1/1642261‑1642717                                                                                                                                                                                                                                           |                                                                                                                                                                                                                               cAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAat                                                                                                                                                                                                                 <  1:168205/250‑1 (MQ=255)     ttCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATcgc                                                                                                                                                                                                            <  1:397485/251‑1 (MQ=255)          cAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGcac                                                                                                                                                                                                       <  1:592240/251‑1 (MQ=255)               tGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATAc                                                                                                                                                                                                  <  1:479270/251‑1 (MQ=255)               tGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATAc                                                                                                                                                                                                  <  1:747832/251‑1 (MQ=255)                  tATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTa                                                                                                                                                                                               <  1:597621/251‑1 (MQ=255)                   aTTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTAc                                                                                                                                                                                              <  1:764240/251‑1 (MQ=255)                       aCCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAAc                                                                                                                                                                                          <  1:522154/251‑1 (MQ=255)                       aCCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAAc                                                                                                                                                                                          <  1:62325/251‑1 (MQ=255)                                 tCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGaaa                                                                                                                                                                                <  1:804946/251‑1 (MQ=255)                                    gcggTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGGAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGGACACGCAGGCTCCAAAAGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATcgc                                                                                                                                                                                                            >  1:604986/1‑220 (MQ=255)                                       gTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAAAGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGt                                                                                                                                                                          >  1:349404/1‑251 (MQ=255)                                                   aaGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCAt                                                                                                                                                               <  1:748817/250‑1 (MQ=255)                                                              aaTATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAAc                                                                                                                                                   <  1:370438/251‑1 (MQ=255)                                                                     gTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAAt                                                                                                                                                                              >  1:91551/1‑217 (MQ=255)                                                                     gTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAAACGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGAt                                                                                                                                            >  1:838764/1‑251 (MQ=255)                                                                       tttGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGTCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATAc                                                                                                                                          <  1:447721/251‑1 (MQ=255)                                                                       tttGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATAc                                                                                                                                          <  1:522477/251‑1 (MQ=255)                                                                         tGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCg                                                                                                                                        >  1:584150/1‑251 (MQ=255)                                                                                   ggTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTAtt                                                                                                                              >  1:277219/1‑251 (MQ=255)                                                                                     tAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCACCTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTg                                                                                                                             <  1:222856/250‑1 (MQ=255)                                                                                     tAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTgg                                                                                                                            <  1:579206/251‑1 (MQ=255)                                                                                           cGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAAt                                                                                                                      >  1:531393/1‑251 (MQ=255)                                                                                           cGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTACCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAAt                                                                                                                      >  1:263744/1‑251 (MQ=255)                                                                                              gAAATACTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTAc                                                                                                                                                                                              <  1:447610/176‑1 (MQ=255)                                                                                                aaTATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTAcgc                                                                                                                 <  1:640489/251‑1 (MQ=255)                                                                                                  tatTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGAc                                                                                                                                                           >  1:141295/1‑207 (MQ=255)                                                                                                          cAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACg                                                                                                       <  1:5302/251‑1 (MQ=255)                                                                                                          cAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATCCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACg                                                                                                       <  1:446096/251‑1 (MQ=255)                                                                                                             cGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCa                                                                                                    >  1:487869/1‑251 (MQ=255)                                                                                                               gTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACa                                                                                                                                                                                             <  1:254362/160‑1 (MQ=255)                                                                                                               gTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCAc                                                                                                                                     <  1:251299/216‑1 (MQ=255)                                                                                                               gTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTg                                                                                                                             >  1:159324/1‑224 (MQ=255)                                                                                                               gTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCAc                                                                                                   >  1:95798/1‑250 (MQ=255)                                                                                                                      ccTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTg                                                                                           >  1:659123/1‑251 (MQ=255)                                                                                                                      ccTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTg                                                                                           <  1:163836/251‑1 (MQ=255)                                                                                                                        tGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCa                                                                                         <  1:266374/251‑1 (MQ=255)                                                                                                                        tGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCa                                                                                         <  1:346726/251‑1 (MQ=255)                                                                                                                         gTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGc                                                                                                     >  1:895504/1‑238 (MQ=255)                                                                                                                         gTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTg                                                                                           <  1:62570/248‑1 (MQ=255)                                                                                                                           cctccGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGGAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACgg                                                                                      >  1:245625/1‑251 (MQ=255)                                                                                                                            ctccGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACggg                                                                                     <  1:422776/251‑1 (MQ=255)                                                                                                                             tccGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATcgc                                                                                                                                                                                                            >  1:728915/1‑131 (MQ=255)                                                                                                                               cGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGgtgt                                                                                  <  1:725427/251‑1 (MQ=255)                                                                                                                                   aaCTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTgttgtt                                                                              <  1:655356/251‑1 (MQ=255)                                                                                                                                   aaCTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTgttgtt                                                                              <  1:884491/251‑1 (MQ=255)                                                                                                                                   aaCTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTgttgtt                                                                              <  1:123560/251‑1 (MQ=255)                                                                                                                                     cTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGtttt                                                                            >  1:490654/1‑251 (MQ=255)                                                                                                                                       tCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTaa                                                                          >  1:320299/1‑251 (MQ=255)                                                                                                                                       tCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTaa                                                                          >  1:900530/1‑251 (MQ=255)                                                                                                                                         ccGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAata                                                                        <  1:435527/251‑1 (MQ=255)                                                                                                                                          cGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAatat                                                                       <  1:266744/251‑1 (MQ=255)                                                                                                                                                tttCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAATATTAGTc                                                                  <  1:89131/250‑1 (MQ=255)                                                                                                                                                tttCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAATATTAGTc                                                                  <  1:831822/250‑1 (MQ=255)                                                                                                                                                 ttCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAata                                                                        <  1:868533/243‑1 (MQ=255)                                                                                                                                                       gCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAatat                                                                       <  1:826237/238‑1 (MQ=255)                                                                                                                                                                    ggCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTg                                                                                           >  1:133524/1‑205 (MQ=255)                                                                                                                                                                    ggCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCAc                                                                                        >  1:121898/1‑208 (MQ=255)                                                                                                                                                                    ggCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAATATTAGTCATGATAAAAAGTGCGGTGGaa                                             >  1:840427/1‑251 (MQ=255)                                                                                                                                                                               cTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGttt                                                                             <  1:197423/208‑1 (MQ=255)                                                                                                                                                                               cTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAata                                                                        <  1:429012/213‑1 (MQ=255)                                                                                                                                                                                      cacaTTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAATATTAGTCATGATAAAAAGTGCGGTGGAAATTCATTGTGTTTTTCCa                           <  1:310851/251‑1 (MQ=255)                                                                                                                                                                                            cACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTgttgtt                                                                              <  1:562679/194‑1 (MQ=255)                                                                                                                                                                                                    ccGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAata                                                                        <  1:567863/192‑1 (MQ=255)                                                                                                                                                                                                      gTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAAt                                                                                                                                                                              <  1:296827/88‑1 (MQ=255)                                                                                                                                                                                                           ggCGTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAatat                                                                       >  1:79754/1‑186 (MQ=255)                                                                                                                                                                                                              gTGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAATATTAGTCATGATAAAAAGTGCGGTGGAAATTCATTGTGTTTTTCCACCGCACtttttt               <  1:532982/239‑1 (MQ=255)                                                                                                                                                                                                               tGCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAATATTAGTCATGATAAAAAGTGCGGTGGAAATTCATTGTGTTTTTCCACCGCACTTTTTTAGTGCGCCTAATc  <  1:259116/251‑1 (MQ=255)                                                                                                                                                                                                                gCTCAATCGCATCAACCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAATATTAGTCATGATAAAAAg                                                       <  1:555704/197‑1 (MQ=255)                                                                                                                                                                                                                             aaCCAGATTTTCGTGGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGc                                                                                                                           <  1:374070/116‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                               CAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAATATCGGTTTTGAAGTGGCAGGTAAATTCGTGAAATATTCCGACAACGGTTCAACCCTGTCCTCCGTCAACTTCCCGGAAGTTTCCCTGCCGGAACACGCAGGCTCCAAACGCTTGTTGCACATTCACGAAAACCGTCCGGGCGTGCTCAATCGCATCAACCAGATTTTCGTAGAAGCCAACGTGAATATCGCCGCACAATACTTACAAACCGACCCGAAAATCGGTTACGTCGTCATTGACGTGGAAACGGAAGATACCGCACCGTTATTGGCGAAATTACGCGAAATCGACGGCACCATTCGTGCACGGGTGTTGTTTTAATATTAGTCATGATAAAAAGTGCGGTGGAAATTCATTGTGTTTTTCCACCGCACTTTTTTAGTGCGCCTAATC  >  1/1642261‑1642717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑