Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,617,894	A→T	L346L (CTA→CTT)	unknown →	GTP‑binding protein TypA/BipA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,617,894	0	A	T	100.0%	218.8 / NA	76	L346L (CTA→CTT)	unknown	GTP‑binding protein TypA/BipA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (40/36);  total (40/36)

ATTTAGGCTTACAACGTTACGAAGAAGACATCTCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTAGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGACATTGAAGAACAACATCAGGGCGCGGTCATGG  >  1/1617647‑1618126                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         aTTTAGGCTTACAACGTTACGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTa                                                                                                                                                                                                                                       >  1:194791/1‑251 (MQ=255)  tttAGGCTTACAACGTTACGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTac                                                                                                                                                                                                                                      <  1:478704/251‑1 (MQ=255)      ggCTTACAACGTTACGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACaa                                                                                                                                                                                                                                  >  1:466504/1‑251 (MQ=255)       gCTTACAACGTTACGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACaa                                                                                                                                                                                                                                  >  1:456106/1‑250 (MQ=255)            cAACGTTACGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTgg                                                                                                                                                                                                                             >  1:497532/1‑250 (MQ=255)              aCGTTACGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTacac                                                                                                                                                                                                                                    <  1:108439/241‑1 (MQ=255)              aCGTTACGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCAt                                                                                                                                                                                                                          <  1:810591/251‑1 (MQ=255)              aCGTTACGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCAt                                                                                                                                                                                                                          <  1:254557/251‑1 (MQ=255)                gTTACGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTCGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTacac                                                                                                                                                                                                                                    <  1:751483/239‑1 (MQ=255)                   aCGAAGAAGACATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTgcgc                                                                                                                                                                                                                     <  1:83166/251‑1 (MQ=255)                        gaagaCATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTaga                                                                                                                                                                                                                >  1:517794/1‑251 (MQ=255)                         aagaCATCGCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTaga                                                                                                                                                                                                                <  1:366833/250‑1 (MQ=255)                                               cATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAAt                                                                                                                                                                                         >  1:667368/1‑251 (MQ=255)                                                 tCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGTCATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAAtt                                                                                                                                                                                        >  1:496022/1‑250 (MQ=255)                                                   gTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCcg                                                                                                                                                                                     >  1:71897/1‑251 (MQ=255)                                                        gATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCAt                                                                                                                                                                                                                          <  1:364903/209‑1 (MQ=255)                                                                                 atTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATc                                                                                                                                                       <  1:597491/251‑1 (MQ=255)                                                                                  tttCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATcc                                                                                                                                                      <  1:727695/251‑1 (MQ=255)                                                                                   ttCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGc                                                                                                                                                                                                                            >  1:701675/1‑180 (MQ=255)                                                                                   ttCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCAGGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCAt                                                                                                                                                            <  1:436882/244‑1 (MQ=255)                                                                                    tCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTc                                                                                                                                                                                       <  1:306001/216‑1 (MQ=255)                                                                                      cGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCgtgt                                                                                                                                                  <  1:417414/251‑1 (MQ=255)                                                                                                       tCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTacac                                                                                                                                                                                                                                    <  1:630329/152‑1 (MQ=255)                                                                                                       tCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCAt                                                                                                                                                                                                                          <  1:274742/162‑1 (MQ=255)                                                                                                       tCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATgcgg                                                                                                                                 >  1:319653/1‑251 (MQ=255)                                                                                                        cAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATc                                                                                                                                                       <  1:170885/228‑1 (MQ=255)                                                                                                        cAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGC‑AATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAAc                                                                                                                                                                                                                                 <  1:241435/153‑1 (MQ=255)                                                                                                               gTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTac                                                                                                                                                                                                                                      <  1:376562/142‑1 (MQ=255)                                                                                                               gTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCa                                                                                                                                                                                                                           >  1:406166/1‑153 (MQ=255)                                                                                                               gTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAgg                                                                                                                         >  1:652244/1‑251 (MQ=255)                                                                                                               gTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAgg                                                                                                                         >  1:373762/1‑251 (MQ=255)                                                                                                               gTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAgg                                                                                                                         >  1:790240/1‑251 (MQ=255)                                                                                                                tAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAgg                                                                                                                         <  1:893062/250‑1 (MQ=255)                                                                                                                      ccTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCATCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGaa                                                                                                                  <  1:289293/251‑1 (MQ=255)                                                                                                                         tGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTa                                                                                                               >  1:680358/1‑251 (MQ=255)                                                                                                                              gCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCg                                                                                                           <  1:574272/250‑1 (MQ=255)                                                                                                                               cATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGt                                                                                                                                                                                                                               <  1:820818/133‑1 (MQ=255)                                                                                                                                   aaGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTcc                                                                                                      >  1:774830/1‑250 (MQ=255)                                                                                                                                     gtgtTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAAcccc                                                                                                                                                                                                       >  1:43330/1‑151 (MQ=255)                                                                                                                                     gtgtTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTcccg                                                                                                    >  1:860609/1‑250 (MQ=255)                                                                                                                                       gtTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTcccgcc                                                                                                  >  1:561590/1‑250 (MQ=255)                                                                                                                                        ttGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCg                                                                                                           >  1:388504/1‑240 (MQ=255)                                                                                                                                        ttGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTcccgcccg                                                                                                >  1:873961/1‑251 (MQ=255)                                                                                                                                        ttGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTcc                                                                                                      <  1:666836/245‑1 (MQ=255)                                                                                                                                              aaCCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGaa                                                                                                                  <  1:360683/227‑1 (MQ=255)                                                                                                                                                      gTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCaa                                                                                  >  1:46297/1‑251 (MQ=255)                                                                                                                                                        cACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGt                                                                                           >  1:25570/1‑240 (MQ=255)                                                                                                                                                         aCCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACa                                                                               >  1:122329/1‑251 (MQ=255)                                                                                                                                                          ccATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGCGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGATAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCCCCCGAAAGTTATCTTCACACaa                                                                              >  1:690301/1‑251 (MQ=255)                                                                                                                                                            aTGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTcc                                                                                                      <  1:187658/225‑1 (MQ=255)                                                                                                                                                              gTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTacac                                                                                                                                                                                                                                    >  1:690212/1‑97 (MQ=255)                                                                                                                                                                   tctGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAAATCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGt                                                                     <  1:869212/251‑1 (MQ=255)                                                                                                                                                                      gCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCAt                                                                  >  1:36069/1‑251 (MQ=255)                                                                                                                                                                       cGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAACCAAGTTGATGGTCATa                                                                 >  1:158350/1‑251 (MQ=255)                                                                                                                                                                        gTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCg                                                                                                                                            >  1:253398/1‑175 (MQ=255)                                                                                                                                                                        gTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATaa                                                                >  1:837671/1‑251 (MQ=255)                                                                                                                                                                            acacCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCAt                                                                                                                                                            >  1:15580/1‑155 (MQ=255)                                                                                                                                                                                 acGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAAcc                                                       <  1:895081/250‑1 (MQ=255)                                                                                                                                                                                  cGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGCGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAAccc                                                      <  1:435887/251‑1 (MQ=255)                                                                                                                                                                                         ttGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAAc                                               <  1:637783/251‑1 (MQ=255)                                                                                                                                                                                         ttGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAAc                                               <  1:207729/251‑1 (MQ=255)                                                                                                                                                                                                   gaagGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGa                                         <  1:380134/247‑1 (MQ=255)                                                                                                                                                                                                    aagGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGAt                                     <  1:575942/250‑1 (MQ=255)                                                                                                                                                                                                     agGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAAc                                                              >  1:819011/1‑224 (MQ=255)                                                                                                                                                                                                      ggCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGa                                  >  1:653858/1‑251 (MQ=255)                                                                                                                                                                                                      ggCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGa                                  >  1:740491/1‑251 (MQ=255)                                                                                                                                                                                                       gCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTcccgcc                                                                                                  >  1:69826/1‑186 (MQ=255)                                                                                                                                                                                                         aaaTATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGACAt                               <  1:817050/251‑1 (MQ=255)                                                                                                                                                                                                           atatGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGACATTg                             >  1:381049/1‑251 (MQ=255)                                                                                                                                                                                                                            aaaTTTTAGAGCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGACATTgaag                          <  1:495188/237‑1 (MQ=255)                                                                                                                                                                                                                                      gCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTcc                                                                                                      >  1:672080/1‑151 (MQ=255)                                                                                                                                                                                                                                      gCGTTTGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGACATTGAAGAACAACATCAGGGCGCGGTCATgg  >  1:897633/1‑251 (MQ=255)                                                                                                                                                                                                                                         tttGAACAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGaa                                                                                                                  >  1:660826/1‑136 (MQ=255)                                                                                                                                                                                                                                               cAAAGAGCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGACATTGAAGAACAACATCAGGgcg           <  1:483618/233‑1 (MQ=255)                                                                                                                                                                                                                                                   gagCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGACATTGAAGAACAACAt                  >  1:682205/1‑222 (MQ=255)                                                                                                                                                                                                                                                    agCTTGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAAc                                                              <  1:108963/177‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         ATTTAGGCTTACAACGTTACGAAGAAGACATCTCCAGCGCCGGCGACATCGTAGCGATTACCGGTTTAGGCGAGCTAAATATTTCCGATACCATCTGCGACATCAATAATGTAGAAGCCTTGCCGGCATTAAGTGTTGATGAACCGACCGTCACCATGTTCTTCTGCGTCAACACCTCGCCGTTTTGTGGCAAAGAAGGCAAATATGTGACTTCCCGCCAAATTTTAGAGCGTTTGAACAAAGAGCTAGTACACAACGTGGCATTGCGCGTAGAAGAAACCCCTAACCCGGATGAATTCCGCGTTTCCGGTCGCGGCGAATTGCATTTATCCGTGTTAATCGAGAACATGCGGCGCGAAGGCTACGAATTAGCCGTGTCCCGCCCGAAAGTTATCTTCAAACAAGTTGATGGTCATAAACAAGAACCCTTTGAACAAGTGACGATTGACATTGAAGAACAACATCAGGGCGCGGTCATGG  >  1/1617647‑1618126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑