Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,613,753	C→A	intergenic (+56/‑236)	unknown → / → unknown	UPF0053 inner membrane protein YgdQ/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,613,753	0	C	A	100.0%	172.1 / NA	62	intergenic (+56/‑236)	unknown/unknown	UPF0053 inner membrane protein YgdQ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/30);  total (32/30)

ATTTTGTTGAAAATCATCCGACATTTAAAATTTTAGCACTCGCCTTCCTGATCCTGATCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTG  >  1/1613507‑1613979                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   aTTTTGTTGAAAATCATCCGACATTTAAAATTTTAGCACTCGCCTTCCTGATCCTGATCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGcaccc                                                                                                                                                                                                                                 >  1:82115/1‑250 (MQ=255)           aaaTCATCCGACATTTAAAATTTTAGCACTCGCCTTCCTGATCCTGATCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGa                                                                                                                                                                                                                      >  1:129579/1‑251 (MQ=255)            aaTCATCCGACATTTAAAATTTTAGCACTCGCCTTCCTGATCCTGATCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGac                                                                                                                                                                                                                     <  1:3492/251‑1 (MQ=255)                      cATTTAAAATTTTAGCACTCGCCTTCCTGATCCTGATCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTg                                                                                                                                                                                                           <  1:5423/251‑1 (MQ=255)                                            cTTCCTGATCCTGATCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTActc                                                                                                                                                                                     <  1:289098/251‑1 (MQ=255)                                            cTTCCTGATCCTGATCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTActc                                                                                                                                                                                     <  1:166408/251‑1 (MQ=255)                                              tcctgatcctgaTCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTc                                                                                                                                                                                           <  1:297216/243‑1 (MQ=255)                                              tcctgatcctgaTCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTActc                                                                                                                                                                                     >  1:712309/1‑249 (MQ=255)                                              tcctgatcctgaTCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCtt                                                                                                                                                                                   >  1:535420/1‑251 (MQ=255)                                                   atcctgatcGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTActc                                                                                                                                                                                     >  1:521471/1‑244 (MQ=255)                                                   atcctgatcGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTActc                                                                                                                                                                                     >  1:854152/1‑244 (MQ=255)                                                   atcctgatcGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATc                                                                                                                                                                                <  1:231593/249‑1 (MQ=255)                                                   atcctgatcGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCaa                                                                                                                                                                              >  1:864776/1‑251 (MQ=255)                                                    tcctgatcGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTc                                                                                                                                                                                           <  1:639912/237‑1 (MQ=255)                                                        gatcGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCg                                                                                                                                                                         >  1:663813/1‑251 (MQ=255)                                                           cGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGttt                                                                                                                                                                      <  1:33338/251‑2 (MQ=255)                                                             gtgtCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTc                                                                                                                                                                                           >  1:196334/1‑228 (MQ=255)                                                             gtgtCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGtttag                                                                                                                                                                    >  1:178204/1‑248 (MQ=255)                                                             gtgtCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGtttag                                                                                                                                                                    >  1:402053/1‑248 (MQ=255)                                                                tCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGtttagaaa                                                                                                                                                                 <  1:3683/251‑7 (MQ=255)                                                                tCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGtttagaaa                                                                                                                                                                 <  1:257214/251‑7 (MQ=255)                                                                   ccTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAAcc                                                                                                                                                                                                                          <  1:676883/191‑1 (MQ=255)                                                                   ccTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAg                                                                                                                                                                                                   <  1:103225/214‑1 (MQ=255)                                                                   ccTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGtttagaaatta                                                                                                                                                              >  1:871108/1‑242 (MQ=255)                                                                         ttATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAAc                                                                                                                                                        <  1:14030/251‑1 (MQ=255)                                                                            tCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAgg                                                                                                                                                     <  1:54277/251‑1 (MQ=255)                                                                                  gCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAAttt                                                                                                                                                                           >  1:520098/1‑223 (MQ=255)                                                                                           tCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGAtt                                                                                                                                      >  1:585994/1‑251 (MQ=255)                                                                                               tgtgCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTgg                                                                                                                                  <  1:895989/251‑1 (MQ=255)                                                                                                         gCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTc                                                                                                                                                                                           >  1:152168/1‑184 (MQ=255)                                                                                                          cGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCG‑AAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGt                                                                                                                      <  1:245913/251‑1 (MQ=255)                                                                                                             atatTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTgg                                                                                                                    >  1:519324/1‑251 (MQ=255)                                                                                                              tatTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTActc                                                                                                                                                                                     >  1:537894/1‑185 (MQ=255)                                                                                                                 ttACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAAc                                                                                                                                                        <  1:581617/211‑1 (MQ=255)                                                                                                                   aCTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAAc                                                                                                                                                                                                                           >  1:329775/1‑142 (MQ=255)                                                                                                                   aCTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCtgaac                                                                                                              <  1:51218/251‑5 (MQ=255)                                                                                                                        gCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTtcat                                                                                                         >  1:470504/1‑251 (MQ=255)                                                                                                                        gCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTtcat                                                                                                         >  1:197995/1‑251 (MQ=255)                                                                                                                                cTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGAttt                                                                                                  >  1:75044/1‑250 (MQ=255)                                                                                                                                  tttCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTActc                                                                                                                                                                                     <  1:400343/165‑1 (MQ=255)                                                                                                                                                     tctcAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTg                                                                            >  1:247082/1‑251 (MQ=255)                                                                                                                                                      ctcAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTgg                                                                           >  1:157598/1‑251 (MQ=255)                                                                                                                                                          aTACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGaaaa                                                                       >  1:248147/1‑251 (MQ=255)                                                                                                                                                          aTACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGCGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGaaaa                                                                       >  1:429464/1‑251 (MQ=18)                                                                                                                                                            aCCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAg                                                                      >  1:518045/1‑250 (MQ=255)                                                                                                                                                                   tGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTg                                                                                                                                                                                                           <  1:234922/110‑1 (MQ=255)                                                                                                                                                                     cGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGAGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGa                                                            >  1:471639/1‑251 (MQ=17)                                                                                                                                                                      gTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGaa                                                           >  1:286532/1‑251 (MQ=255)                                                                                                                                                                          aCGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAagag                                                       <  1:121836/251‑1 (MQ=255)                                                                                                                                                                           cGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAag                                                         >  1:212050/1‑248 (MQ=255)                                                                                                                                                                                     cTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATgg                                             <  1:292611/250‑1 (MQ=255)                                                                                                                                                                                                  aaaCAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCtt                               >  1:249735/1‑251 (MQ=255)                                                                                                                                                                                                  aaaCAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCtt                               >  1:566179/1‑251 (MQ=255)                                                                                                                                                                                                  aaaCAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCt                                <  1:465716/250‑1 (MQ=255)                                                                                                                                                                                                    aCAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTtctc                                                                                               <  1:764170/185‑1 (MQ=17)                                                                                                                                                                                                     cAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtata                            <  1:535620/251‑1 (MQ=255)                                                                                                                                                                                                      aaaaGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                           <  1:657698/251‑1 (MQ=255)                                                                                                                                                                                                             cGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAccc                     <  1:342099/250‑1 (MQ=255)                                                                                                                                                                                                               gTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAAc                                                                                                                                                        <  1:810272/117‑1 (MQ=17)                                                                                                                                                                                                                        cacaTTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTaa         >  1:150356/1‑251 (MQ=255)                                                                                                                                                                                                                          caTTAAATTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTaaaa       >  1:46503/1‑251 (MQ=255)                                                                                                                                                                                                                               aaTTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTg  <  1:336185/251‑1 (MQ=255)                                                                                                                                                                                                                               aaTTTCAACCGCACTTTGCTCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTg  <  1:688941/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   ATTTTGTTGAAAATCATCCGACATTTAAAATTTTAGCACTCGCCTTCCTGATCCTGATCGGTGTCACCTTAATTATCGAGAGCTTCAGCATCCATGTGCCGAAAGCGTATATTTACTCCGCTATGGGCTTTTCCGTATTTGTGGAAATTCTCAATACCCAAATGCGTAAACGCTTGGGCGCTAAAATTTAGAGAAACAAAAGTGCGGTCGAAAAACACATTAAATTTCAACCGCACTTTGCTCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTG  >  1/1613507‑1613979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑