Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,594,371	G→T	L61I (CTT→ATT)	unknown ←	Bacterial/Archaeal Transporter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,594,371	0	G	T	100.0%	233.2 / NA	81	L61I (CTT→ATT)	unknown	Bacterial/Archaeal Transporter family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (37/44);  total (37/44)

AATGTGTTTTTTAAAACGCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAAGATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAAC  >  1/1594125‑1594598                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    aaTGTGTTTTTTAAAACGCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGt                                                                                                                                                                                                                                 <  1:420167/251‑1 (MQ=255) aaTGTGTTTTTTAAAACGCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGt                                                                                                                                                                                                                                 <  1:854532/251‑1 (MQ=255)  aTGTGTTTTTTAAAACGCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGtt                                                                                                                                                                                                                                <  1:179979/251‑1 (MQ=255)    gtgtTTTTTAAAACGCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGa                                                                                                                                                                                                                              >  1:326617/1‑251 (MQ=255)       ttttttAAAACGCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAgg                                                                                                                                                                                                                            <  1:414858/250‑1 (MQ=255)             aaaaCGCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGc                                                                                                                                                                                                                     <  1:14615/251‑1 (MQ=255)                 cGCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCaa                                                                                                                                                                                                                         <  1:272250/243‑1 (MQ=255)                  gCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCcatt                                                                                                                                                                                                                 >  1:243339/1‑250 (MQ=255)                                 cccGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGaaa                                                                                                                                                                                                  <  1:308063/250‑1 (MQ=255)                                        gAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACg                                                                                                                                                                                                          <  1:666180/235‑1 (MQ=255)                                                         atcaatAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAAt                                                                                                                                                                         >  1:664495/1‑251 (MQ=255)                                                           caatAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGtt                                                                                                                                                                                                                                >  1:18797/1‑194 (MQ=255)                                                               aatTTCATACTCACCGGCTCGCCCAAGATATTCACACCTAACACAAAGGCAAGAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAATTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTGCGCATCGAAATAATCGCCACAATCACAAATAAAATCactac                                                                                                                                                                   <  1:380760/251‑1 (MQ=255)                                                               aatTTCATACTCACCGGCTCGCCCAAGATAGTCACTCCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCactac                                                                                                                                                                   <  1:670170/251‑1 (MQ=255)                                                                atTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATaaaa                                                                                                                                                                          >  1:447338/1‑243 (MQ=255)                                                                    cATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTAc                                                                                                                                                              <  1:219911/251‑1 (MQ=255)                                                                    cATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTAc                                                                                                                                                              <  1:232155/251‑1 (MQ=255)                                                                    cATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTAc                                                                                                                                                              <  1:396773/251‑1 (MQ=255)                                                                     aTACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGc                                                                                                                                                                                                                     <  1:529551/195‑1 (MQ=255)                                                                         tCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATa                                                                                                                                                         <  1:651230/251‑1 (MQ=255)                                                                               gCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGt                                                                                                                                                    <  1:15916/250‑1 (MQ=255)                                                                                    cccAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCactac                                                                                                                                                                   <  1:177020/230‑1 (MQ=255)                                                                                        aGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGaa                                                                                                                                                           >  1:847980/1‑234 (MQ=255)                                                                                        aGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTa                                                                                                                                          >  1:244150/1‑251 (MQ=255)                                                                                           tAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTa                                                                                                                                       >  1:603308/1‑251 (MQ=255)                                                                                                 cacCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAaataa                                                                                                                                                                                               >  1:395264/1‑189 (MQ=255)                                                                                                 cacCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGt                                                                                                                                                    >  1:153500/1‑232 (MQ=255)                                                                                                 cacCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACACAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTAc                                                                                                                                         <  1:745357/243‑1 (MQ=255)                                                                                                       acacAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTc                                                                                                                            >  1:728936/1‑250 (MQ=255)                                                                                                         acaATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCcac                                                                                                                         <  1:763039/251‑1 (MQ=255)                                                                                                          caATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCcaca                                                                                                                        >  1:806109/1‑251 (MQ=255)                                                                                                              ggCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGc                                                                                                                                                                                                                           >  1:838717/1‑148 (MQ=255)                                                                                                              ggCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATc                                                                                                                                                                                                      <  1:232840/169‑1 (MQ=255)                                                                                                              ggCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTAc                                                                                                                                                              >  1:353050/1‑209 (MQ=255)                                                                                                              ggCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTa                                                                                                                    >  1:418496/1‑251 (MQ=255)                                                                                                              ggCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTa                                                                                                                    >  1:118370/1‑251 (MQ=255)                                                                                                              ggCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTa                                                                                                                    >  1:898773/1‑251 (MQ=255)                                                                                                              ggCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTa                                                                                                                    >  1:342909/1‑251 (MQ=255)                                                                                                                cAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTg                                                                                                                                                                 >  1:189617/1‑204 (MQ=255)                                                                                                                 aaTAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAAtt                                                                                                                 <  1:447636/251‑1 (MQ=255)                                                                                                                   tAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAgg                                                                                                                                                                                                                            <  1:629630/142‑1 (MQ=255)                                                                                                                   tAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTg                                                                                                               >  1:687592/1‑251 (MQ=255)                                                                                                                             gAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAAt                                                                                                       <  1:33247/249‑1 (MQ=255)                                                                                                                               gttcaTCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGGAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGcc                                                                                                                               <  1:453589/219‑1 (MQ=255)                                                                                                                               gTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGc                                                                                                    >  1:680145/1‑250 (MQ=255)                                                                                                                               gTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGc                                                                                                    >  1:212181/1‑250 (MQ=255)                                                                                                                                tttATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCg                                                                                                  <  1:483947/251‑1 (MQ=255)                                                                                                                                     cAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATaaa                                                                                                                                                       >  1:479947/1‑193 (MQ=255)                                                                                                                                     cAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGcc                                                                                                                               >  1:734418/1‑217 (MQ=255)                                                                                                                                     cAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAAt                                                                                             >  1:497467/1‑251 (MQ=255)                                                                                                                                     cAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAAt                                                                                             >  1:478460/1‑251 (MQ=255)                                                                                                                                      aaTAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATc                                                                                            <  1:731231/251‑1 (MQ=255)                                                                                                                                                aCCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCactac                                                                                                                                                                   >  1:568015/1‑170 (MQ=255)                                                                                                                                                aCCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCaaaaaa                                                                                  >  1:436408/1‑251 (MQ=255)                                                                                                                                                aCCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCaaaaaa                                                                                  >  1:32819/1‑251 (MQ=255)                                                                                                                                                   cAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAGAAATg                                                                                >  1:442436/1‑250 (MQ=255)                                                                                                                                                        tGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAAc                                                                          <  1:198724/251‑1 (MQ=255)                                                                                                                                                                         aaGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGAcc                                                         <  1:60697/251‑1 (MQ=255)                                                                                                                                                                              cgATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAAtt                                                    <  1:615920/251‑1 (MQ=255)                                                                                                                                                                                aTAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTaa                                                                                                                   >  1:575584/1‑186 (MQ=255)                                                                                                                                                                                aTAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTc                                                   <  1:445094/250‑1 (MQ=255)                                                                                                                                                                                    tAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCactac                                                                                                                                                                   >  1:791431/1‑134 (MQ=255)                                                                                                                                                                                    tAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCAtttt                                              <  1:875707/251‑1 (MQ=255)                                                                                                                                                                                       cAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAAt                                           <  1:381057/251‑1 (MQ=255)                                                                                                                                                                                                  aaaTCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGtt                                <  1:694012/251‑1 (MQ=255)                                                                                                                                                                                                  aaaTCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGtt                                <  1:421752/251‑1 (MQ=255)                                                                                                                                                                                                  aaaTCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGtt                                <  1:175572/251‑1 (MQ=255)                                                                                                                                                                                                     tCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTc                                                   <  1:179049/229‑1 (MQ=255)                                                                                                                                                                                                               aCCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATa                                                                                                                                                         >  1:853630/1‑117 (MQ=255)                                                                                                                                                                                                                 cccGGATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCt                 <  1:489205/251‑1 (MQ=255)                                                                                                                                                                                                                    ggATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTc                                                                                                                                    <  1:244671/133‑1 (MQ=255)                                                                                                                                                                                                                    ggATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGAccc                                                        >  1:865494/1‑209 (MQ=255)                                                                                                                                                                                                                    ggATAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTta              >  1:166854/1‑251 (MQ=255)                                                                                                                                                                                                                       tAACACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTataa           <  1:210683/251‑1 (MQ=255)                                                                                                                                                                                                                        aaCACAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTataat          <  1:259853/251‑1 (MQ=255)                                                                                                                                                                                                                         acacAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCcattcat                                                                                                                                                                                                              >  1:229025/1‑54 (MQ=255)                                                                                                                                                                                                                         acacAAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCcac                                                                                                                         <  1:257281/139‑1 (MQ=255)                                                                                                                                                                                                                            caAGGAAAATAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGtt                                >  1:117488/1‑225 (MQ=255)                                                                                                                                                                                                                                 aaaaTAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAAc  <  1:88177/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaaaTAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAAc  <  1:356103/250‑1 (MQ=255)                                                                                                                                                                                                                                   aaTAACCGGCTTGGTCGCAATATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAAtc                                          <  1:676579/208‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    AATGTGTTTTTTAAAACGCCAAGACCAACACGCCCGAAAGAATCAATCCTGCCCCAATCAATAATTTCATACTCACCGGCTCGCCCAAGATAGTCACACCTAACACAATGGCAATAACCACGCTGAGTTTATCAATAGGTGCGACCCAAGATGCCGGCGCAAGTTGTAAAGCGCGATAGTAACAAAGCCAGGAAAATCCCGTCGCCACCCCGGATAACACAAGGAAAATAACCGGCTTGGTCGCAAGATGTTGAGGCAATTGCCATTCATTACGCATCGAAATAATCGCCACAATCACAAATAAAATCACTACTGTACGAATAAATGTCGCTAAATTACTATTCAAGCCTTCCACACCTAATTTGCCTAAAATTGCCGTTAATCCGGCAAAAAATGCGGAACCGATCGCAAACCAGACCCAATTCATTTTAATCTCCTCATGTTTCTGCAATATTTCCTTTATAATATACCAAC  >  1/1594125‑1594598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑