Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,591,045 T→C H248R (CAC→CGC)  unknown ← Guanosine‑3',5'‑bis(diphosphate) 3'‑pyrophosphohydrolase (EC 3.1.7.2) / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,591,0450TC100.0% 224.0 / NA 76H248R (CAC→CGC) unknownGuanosine‑3',5'‑bis(diphosphate) 3'‑pyrophosphohydrolase (EC 3.1.7.2) / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/28);  total (48/28)

ATTTGATCCATTTCTTCGGTACGAATTTGCACTTCCACCGGCACGCCGTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGTGCATTTTTTGGTAGATGGCATACAAATGCTTTTCACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTG  >  1/1590799‑1591288
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
aTTTGATCCATTTCTTCGGTACGAATTTGCACTTCCACCGGCACGCCGTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCAt                                                                                                                                                                                                                                                 >  1:508443/1‑251 (MQ=255)
        cATTTCTTCGGTACGAATTTGCACTTCCACCGGCACGCCGTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGt                                                                                                                                                                                                                                         >  1:410401/1‑251 (MQ=255)
               tcGGTACGAATTTGCACTTCCACCGGCACGCCGTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGc                                                                                                                                                                                                                                  <  1:417057/251‑1 (MQ=255)
                       aaTTTGCACTTCCACCGGCACGCCGTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAAt                                                                                                                                                                                                                          >  1:685983/1‑251 (MQ=255)
                              aCTTCCACCGGCACGCCGTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCttttt                                                                                                                                                                                                                   >  1:508944/1‑250 (MQ=255)
                                               gTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCcgcg                                                                                                                                                                                                  >  1:719484/1‑251 (MQ=255)
                                                        aaTCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACaa                                                                                                                                                                                                                            <  1:340428/216‑1 (MQ=255)
                                                                    aTGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCa                                                                                                                                                                             >  1:476598/1‑251 (MQ=255)
                                                                     tGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGAt                                                                                                                                                                                                                                     <  1:303932/194‑1 (MQ=255)
                                                                     tGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACAt                                                                                                                                                                                   <  1:700758/244‑1 (MQ=255)
                                                                     tGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCaa                                                                                                                                                                            >  1:462924/1‑251 (MQ=255)
                                                                           gACTGATAGCCATTGGCTTTCGGCACGGTAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACaaa                                                                                                                                                                                                                           <  1:451877/198‑1 (MQ=255)
                                                                            aCTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGcc                                                                                                                                                                       >  1:99793/1‑249 (MQ=255)
                                                                             cTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCa                                                                                                                                                                             <  1:704302/242‑1 (MQ=255)
                                                                                 tAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGAt                                                                                                                                                                <  1:885516/251‑1 (MQ=255)
                                                                                         ggCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCgg                                                                                                                                                        >  1:850191/1‑251 (MQ=255)
                                                                                                      gcaATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATAc                                                                                                                                                                                         >  1:121963/1‑205 (MQ=255)
                                                                                                      gcaATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACAt                                                                                                                                                                                   >  1:346955/1‑211 (MQ=255)
                                                                                                         atatAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATACTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCAt                                                                                                                                        >  1:435034/1‑251 (MQ=255)
                                                                                                                cTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCttttta                                                                                                                                                                                                                  <  1:462562/170‑3 (MQ=255)
                                                                                                                          acccgGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTc                                                                                                                                     <  1:176756/237‑1 (MQ=255)
                                                                                                                          acccgGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACcg                                                                                                                        >  1:550091/1‑250 (MQ=255)
                                                                                                                                     gTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTg                                                                                                                                                                                                                                           <  1:770807/124‑1 (MQ=255)
                                                                                                                                     gTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACaaa                                                                                                                                                                                                                           >  1:506726/1‑140 (MQ=255)
                                                                                                                                        tataAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACg                                                                                                         >  1:597052/1‑251 (MQ=255)
                                                                                                                                              ggCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACtttt                                                                                                   >  1:574226/1‑251 (MQ=255)
                                                                                                                                               gCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTc                                                                                                                                     >  1:518560/1‑216 (MQ=255)
                                                                                                                                               gCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTAc                                                                                                                          <  1:566737/227‑1 (MQ=255)
                                                                                                                                                    gCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACtt                                                                                                     >  1:600424/1‑243 (MQ=255)
                                                                                                                                                    gCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCaaa                                                                                             >  1:81370/1‑251 (MQ=255)
                                                                                                                                                    gCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTCTTGGCAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCTTTCAATCATGTCTTTGCGATTACCCCCCGCAATCTGACCGCCTTTTGGCaaa                                                                                             >  1:685426/1‑251 (MQ=255)
                                                                                                                                                      aTTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAAc                                                                                           <  1:565457/251‑1 (MQ=255)
                                                                                                                                                                gCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAAc                                                                                   <  1:332959/249‑1 (MQ=255)
                                                                                                                                                                  acacGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCa                                                                                                                                                                             >  1:610913/1‑157 (MQ=255)
                                                                                                                                                                   cacGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATcc                                                                                                                                                                                 >  1:731225/1‑152 (MQ=255)
                                                                                                                                                                   cacGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTg                                                                               >  1:156753/1‑250 (MQ=255)
                                                                                                                                                                   cacGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTg                                                                               >  1:55834/1‑250 (MQ=255)
                                                                                                                                                                     cGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTAc                                                                                                                          <  1:597928/205‑1 (MQ=255)
                                                                                                                                                                     cGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTa                                                                                                           <  1:328831/220‑1 (MQ=255)
                                                                                                                                                                     cGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCgg                                                                            >  1:795504/1‑251 (MQ=255)
                                                                                                                                                                             ggTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATgg                                                                    >  1:129726/1‑251 (MQ=255)
                                                                                                                                                                              gTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATc                                                                                                               <  1:830558/207‑1 (MQ=255)
                                                                                                                                                                              gTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATgg                                                                    <  1:43460/250‑1 (MQ=255)
                                                                                                                                                                                 tCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGAt                                                                                                                             >  1:415989/1‑190 (MQ=255)
                                                                                                                                                                                         gttCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATAc                                                                                                                                                                                         >  1:292577/1‑122 (MQ=255)
                                                                                                                                                                                         gttCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTAc                                                                                                                          <  1:20843/185‑1 (MQ=255)
                                                                                                                                                                                         gttCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCaa                                                        >  1:62591/1‑251 (MQ=255)
                                                                                                                                                                                           tCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGa                                                      >  1:16496/1‑251 (MQ=255)
                                                                                                                                                                                                 cGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTcc                                                <  1:253913/251‑1 (MQ=255)
                                                                                                                                                                                                   gTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGa                                                                           >  1:471004/1‑222 (MQ=255)
                                                                                                                                                                                                   gTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAg                                              >  1:400510/1‑251 (MQ=255)
                                                                                                                                                                                                    tGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATGCATGGCTTCAAAACCAAGATCTTCCAg                                              <  1:741625/250‑1 (MQ=255)
                                                                                                                                                                                                           cgcgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTc                                                                                                                                     >  1:846325/1‑156 (MQ=255)
                                                                                                                                                                                                            gcgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATc                                                    >  1:110513/1‑236 (MQ=255)
                                                                                                                                                                                                            gcgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGttttt                                     >  1:856662/1‑251 (MQ=255)
                                                                                                                                                                                                            gcgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGttttt                                     >  1:80084/1‑251 (MQ=255)
                                                                                                                                                                                                             cgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACa                                                                       >  1:56064/1‑216 (MQ=255)
                                                                                                                                                                                                             cgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGtt                                            >  1:398731/1‑243 (MQ=255)
                                                                                                                                                                                                               tAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGAc                                                                                                       >  1:830507/1‑182 (MQ=255)
                                                                                                                                                                                                               tAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGAt                                  >  1:466963/1‑251 (MQ=255)
                                                                                                                                                                                                                    atCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATTCCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATc                                                                                                               <  1:118447/169‑1 (MQ=255)
                                                                                                                                                                                                                      ccATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTa                                                                                                           <  1:662275/171‑1 (MQ=255)
                                                                                                                                                                                                                      ccATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGt                                                                                 <  1:853690/197‑1 (MQ=255)
                                                                                                                                                                                                                         tAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAg                                              >  1:215444/1‑229 (MQ=255)
                                                                                                                                                                                                                           aTGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATcc                       >  1:706129/1‑250 (MQ=255)
                                                                                                                                                                                                                             ggAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCa                    >  1:257088/1‑251 (MQ=255)
                                                                                                                                                                                                                              gAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCaa                   >  1:357349/1‑251 (MQ=255)
                                                                                                                                                                                                                              gAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCaa                   >  1:674996/1‑251 (MQ=255)
                                                                                                                                                                                                                              gAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGC‑AACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGAtg                                 >  1:336593/1‑236 (MQ=255)
                                                                                                                                                                                                                               aaTGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAg                  <  1:48880/251‑1 (MQ=255)
                                                                                                                                                                                                                               aaTGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAg                  <  1:306005/251‑1 (MQ=255)
                                                                                                                                                                                                                                aTGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAg                  >  1:251912/1‑250 (MQ=255)
                                                                                                                                                                                                                                aTGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGc                 <  1:322139/251‑1 (MQ=255)
                                                                                                                                                                                                                                            aTCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAAc      <  1:416900/250‑1 (MQ=255)
                                                                                                                                                                                                                                             tCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACgg    <  1:409065/251‑1 (MQ=255)
                                                                                                                                                                                                                                                tttAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACggcg  >  1:901830/1‑248 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
ATTTGATCCATTTCTTCGGTACGAATTTGCACTTCCACCGGCACGCCGTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGTGCATTTTTTGGTAGATGGCATACAAATGCTTTTCACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTG  >  1/1590799‑1591288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑