Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,588,297	C→G	R456T (AGG→ACG)	unknown ←	ATP‑dependent DNA helicase RecG (EC 3.6.4.12)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,588,297	0	C	G	100.0%	205.4 / NA	72	R456T (AGG→ACG)	unknown	ATP‑dependent DNA helicase RecG (EC 3.6.4.12)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (36/36);  total (36/36)

CGCTTGTTTTTCATTCGGTTTCATTCGCCCGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCCTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCC  >  1/1588062‑1588536                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                cGCTTGTTTTTCATTCGGTTTCATTCGCCCGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCa                                                                                                                                                                                                                                  >  1:532871/1‑251 (MQ=255)                        ttCGCCCGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATcc                                                                                                                                                                                                           <  1:867369/251‑1 (MQ=255)                            cccGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCaaa                                                                                                                                                                                                        <  1:657107/250‑1 (MQ=255)                            cccGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAAt                                                                                                                                                                                                       >  1:458444/1‑251 (MQ=255)                            cccGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAAt                                                                                                                                                                                                       >  1:799632/1‑251 (MQ=255)                            cccGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAAt                                                                                                                                                                                                       >  1:657196/1‑251 (MQ=255)                             ccGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATc                                                                                                                                                                                                      <  1:360319/251‑1 (MQ=255)                              cGTGTCCCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATc                                                                                                                                                                                                      <  1:836808/250‑1 (MQ=255)                              cGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATc                                                                                                                                                                                                      >  1:248040/1‑250 (MQ=255)                                 gtACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATc                                                                                                                                                                                                      >  1:515505/1‑247 (MQ=255)                                 gtACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCa                                                                                                                                                                                                  >  1:287585/1‑251 (MQ=255)                                 gtACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCa                                                                                                                                                                                                  >  1:608056/1‑251 (MQ=255)                                         ccGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCg                                                                                                                                                                                           <  1:213823/250‑1 (MQ=255)                                          cGATGCGTAACTGCGCTAAAATTTTACAGCGTTCTTCCGCCACCCCTTCCGCCTCCTGCGCCTCCAACATTGCCGATTCATCAATCAGAGTGAAAACCCAATAAACCTTCCGTTTTTCCTACGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACTATCTTCGGAAATTGCTATGGTGGTAATCTGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTc                                                                                                                                                                                         <  1:152051/251‑1 (MQ=255)                                              gCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATgg                                                                                                                                                                                     >  1:548074/1‑251 (MQ=255)                                                 tAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCa                                                                                                                                                                                   <  1:628435/250‑1 (MQ=255)                                                             aaTTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTgg                                                                                                                                                                        <  1:402680/249‑1 (MQ=255)                                                               ttttACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATc                                                                                                                                                                    <  1:379168/251‑1 (MQ=255)                                                                                 ccACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCgg                                                                                                                                                          >  1:197212/1‑243 (MQ=255)                                                                                 ccACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGAtt                                                                                                                                                  <  1:830212/251‑1 (MQ=255)                                                                                    ccGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAAc                                                                                                                                               <  1:379179/251‑1 (MQ=255)                                                                                     cGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACt                                                                                                                                              <  1:542961/251‑1 (MQ=255)                                                                                       cTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACtg                                                                                                                                             <  1:773271/250‑1 (MQ=255)                                                                                         tCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACtgatg                                                                                                                                          <  1:383660/251‑1 (MQ=255)                                                                                                                       tcatcaATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATacac                                                                                                                                 <  1:580284/230‑1 (MQ=255)                                                                                                                               caGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTTCCgcagca                                                                                                    >  1:870530/1‑251 (MQ=255)                                                                                                                                 gagTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGGTAAACGCCCGCCTGGTTACCTTTTTCCCGCAGCATg                                                                                                  >  1:817895/1‑251 (MQ=255)                                                                                                                                          cccAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAAc                                                                                                                                               >  1:298594/1‑197 (MQ=255)                                                                                                                                          cccAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTTCCGCAGCATGAGTCGTTgg                                                                                         >  1:533390/1‑251 (MQ=255)                                                                                                                                          cccAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTgg                                                                                         >  1:878180/1‑251 (MQ=255)                                                                                                                                            cAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACtgatg                                                                                                                                          <  1:50460/200‑1 (MQ=255)                                                                                                                                               tAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCAc                                                                                    <  1:772239/251‑1 (MQ=255)                                                                                                                                                 aGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACg                                                                                   >  1:885091/1‑250 (MQ=255)                                                                                                                                                       ccGTTTTTCCTGCGTACGCTCCACATACACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAAAGGTGGTAATCGACGTGCGCCCCGGTGTCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTTCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAAc                                                                            <  1:698185/251‑1 (MQ=255)                                                                                                                                                       ccGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAAc                                                                            <  1:50616/251‑1 (MQ=255)                                                                                                                                                       ccGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAAc                                                                            <  1:502236/251‑1 (MQ=255)                                                                                                                                                       ccGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCCGCCTGATTACCTTTTTTCCGCAGCATGAGTCGTTGGt                                                                                        >  1:822826/1‑239 (MQ=255)                                                                                                                                                                    gTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTAc                                                                                                                   <  1:47756/199‑1 (MQ=255)                                                                                                                                                                       cacGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTcaac                                                            <  1:637901/251‑1 (MQ=255)                                                                                                                                                                       cacGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTcaac                                                            <  1:690535/251‑1 (MQ=255)                                                                                                                                                                             acaTTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACgcctgcc                                                                                                                          >  1:466302/1‑183 (MQ=255)                                                                                                                                                                             acaTTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACggggc                                                                       >  1:680788/1‑231 (MQ=255)                                                                                                                                                                                   ccccGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTGTCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCt                                                <  1:139955/250‑1 (MQ=255)                                                                                                                                                                                        ttCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGcc                                                                                 <  1:273232/213‑1 (MQ=255)                                                                                                                                                                                        ttCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAAc                                           <  1:140821/251‑1 (MQ=255)                                                                                                                                                                                         tCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATTATTAACTGATGAGGATACACGCCCGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTTGTGCACGCCGAAACGGTGCTGTTCGGCAACAATCACCCAACTCAAACg                                          >  1:572579/1‑251 (MQ=255)                                                                                                                                                                                         tCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCttttttc                                                                                                           >  1:318611/1‑184 (MQ=255)                                                                                                                                                                                         tCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACg                                          <  1:137392/251‑1 (MQ=255)                                                                                                                                                                                           gATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGGTTAAATGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCCGCATGAGTCGTTGGGGCCCGCCGAAACGGGGCTGTTCGGCAACAATCACCAAACTCAAACGAt                                        >  1:740990/1‑251 (MQ=255)                                                                                                                                                                                              aaTCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTgg                                                                                         <  1:561647/199‑1 (MQ=255)                                                                                                                                                                                                tctcTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTgg                                                                                                                                                             <  1:351752/129‑1 (MQ=255)                                                                                                                                                                                                tctcTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACgcctgcct                                                                                                                         <  1:856974/165‑1 (MQ=255)                                                                                                                                                                                                tctcTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTTCCGCCGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAAt                                   >  1:8486/1‑251 (MQ=255)                                                                                                                                                                                                tctcTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTTCCGCAGCATGAGTCGTTGGGGCACGCCGAAACGGTGCTGTTCGTCCACAAACACCAAGCTCAAACGATGaaaa                                   >  1:136123/1‑250 (MQ=255)                                                                                                                                                                                                tctcTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAAt                                   <  1:731047/251‑1 (MQ=255)                                                                                                                                                                                                tctcTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGGCGGTGGTGCCCGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCCAACGATGaaaa                                   >  1:178976/1‑250 (MQ=255)                                                                                                                                                                                                tctcTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCCGCCTGATTGCCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGGGCTGTTCGTCAACAAACACCAAGCTCAAACGATGAAAt                                   >  1:445289/1‑251 (MQ=255)                                                                                                                                                                                                   ctGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTc                                                                                              <  1:894830/189‑1 (MQ=255)                                                                                                                                                                                                                 tCGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGaa                  <  1:795871/251‑1 (MQ=255)                                                                                                                                                                                                                  cGGAAATTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAAt                 <  1:598745/251‑1 (MQ=255)                                                                                                                                                                                                                       aTTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGGGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAAcg             >  1:326849/1‑250 (MQ=255)                                                                                                                                                                                                                       aTTGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGGGCACGCCGAAACGGGGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAAcg             >  1:732095/1‑250 (MQ=255)                                                                                                                                                                                                                        ttGCAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCCTGATTAACTGATGAGGATACACGCCCGCCTGATTACCTTTTTCCCGCAGCCTGAGTCGTTGGTGCACGCCCCAACGGTGCTTTTCGTCAACAATCACCCAGCTCCAACGATGAAATTCCACCTCTTCCTTGAATAAcccg           >  1:779073/1‑248 (MQ=255)                                                                                                                                                                                                                           cAATGGTGGTAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGAt                                                                                                                                     <  1:576291/126‑1 (MQ=255)                                                                                                                                                                                                                               ggtggtAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTgg                                                                                                                                                                        >  1:317414/1‑87 (MQ=255)                                                                                                                                                                                                                               ggtggtAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCTTGATTAAATGATGAGGATACACg                                                                                                                                >  1:565579/1‑127 (MQ=255)                                                                                                                                                                                                                                gtggtAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCttt                                                                                                               <  1:637129/143‑1 (MQ=255)                                                                                                                                                                                                                                gtggtAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGGGCTGTTCGTCAACAATCACCAAGCTCAAAAGATGAAATTCCACCTCTTCCTGGAATAAAGCGTGCGTTc    >  1:11685/1‑250 (MQ=255)                                                                                                                                                                                                                                 tggtAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGGCGTTGGTGCACACCCAAACGGTGCTGTTCGTCAACAAACACCAAGCTCAAACGATGAAAATCCACCTCTTCCTGGAATAACGCGTGCGTTcc   >  1:425975/1‑250 (MQ=255)                                                                                                                                                                                                                                  ggtAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATg                                                                                                                                                     >  1:330777/1‑103 (MQ=255)                                                                                                                                                                                                                                  ggtAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGGTACACGCCTGCCTGGTTACCTTTTTCCCGCAGCATGAGTCGTTGGGGCACGCCGAAACGGTGCTGTTCGTCAACAAACACCAAGCTCAAACGAAGAAAATCCACCTCTTCCTGGAATAACGCGGGCGGTccc  >  1:847834/1‑250 (MQ=255)                                                                                                                                                                                                                                  ggtAATCGGCGTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAAAGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAAAGCGTGCGTTccc  >  1:761292/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                CGCTTGTTTTTCATTCGGTTTCATTCGCCCGTGTACCAAGCCGATGCGTAAGTGCGGTAGAATTTTACGGAGATCTTCCGCCACCGCTTCCGCCGCCTGCGCCTCCAACATTTCCGATTCATCAATCAGAGTGCAAACCCAATAAGCCTGCCGTTTTTCCTGCGTACACGCCACATTCACCCGTTCGATAATCTCTGCACGACGATCTTCGGAAATTGCAATGGTGGTAATCGGCCTGCGCCCCGGTGGCAATTCGTCGATAATCGAAGTATCCAAATCCGCATACACCGTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCC  >  1/1588062‑1588536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑