Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,580,534	T→G	intergenic (+169/+471)	unknown → / ← unknown	Na+/H+ antiporter/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,580,534	0	T	G	100.0%	95.3 / NA	47	intergenic (+169/+471)	unknown/unknown	Na+/H+ antiporter/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/19);  total (28/19)

AATCGTCATTGGCTTTACTTACCATTGACTTATCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGAGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTTTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTCCGCTCTTCGCTT  >  1/1580397‑1580779                                                                                                                                          |                                                                                                                                                                                                                                                      aaTCGTCATTGGCTTTACTTACCATTGACTTATCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCAt                                                                                                                                      <  1:299370/251‑1 (MQ=14)            gCTTTACTTACCATTGACTTATCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTActc                                                                                                                                                                 <  1:385083/213‑1 (MQ=17)                    tacCATTGACTTATCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCAc                                                                                                                   <  1:586531/251‑1 (MQ=14)                      ccATTGACTTATCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCacac                                                                                                                                            >  1:408285/1‑224 (MQ=17)                           gACTTATCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACgg                                                                                                                           >  1:228500/1‑236 (MQ=17)                           gACTTATCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAg                                                                                                            >  1:595064/1‑251 (MQ=14)                             cTTATCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGtt                                                                                                          >  1:530600/1‑251 (MQ=14)                                aTCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTcaca                                                                                                                                                            <  1:894339/198‑1 (MQ=17)                                   ctcctATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAAc                                                                                                                                                                                                                           >  1:372163/1‑132 (MQ=2)                                   ctcctATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATc                                                                                                                                                                                                   <  1:843058/156‑1 (MQ=16)                                     cctATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTCCGGttcgt                                                                                                  >  1:422385/1‑247 (MQ=9)                                        atatCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGttt                                                                                               >  1:433355/1‑251 (MQ=14)                                          atCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCa                                                                                             >  1:740939/1‑251 (MQ=14)                                               gAATTTGGGTTGTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGccc                                                                                                                                                 >  1:299628/1‑194 (MQ=17)                                                          gTCAGCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGttt                                                                                               >  1:19191/1‑233 (MQ=17)                                                              gCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATgg                                                                                                                                                         >  1:876551/1‑171 (MQ=17)                                                              gCCTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAgg                                                                                           <  1:350422/233‑1 (MQ=17)                                                               ccTTATTCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTAt                                                                          <  1:616378/249‑1 (MQ=17)                                                                    ttCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTcaca                                                                                                                                                            <  1:130292/162‑1 (MQ=18)                                                                    ttCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTAt                                                                                                    <  1:32327/218‑1 (MQ=17)                                                                    ttCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATcgccgc                                                                    >  1:22129/1‑250 (MQ=17)                                                                    ttCTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTAGTACCACCGCACTATCgccgcc                                                                   <  1:497672/251‑1 (MQ=14)                                                                      cTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGc                                                                                                                                                                                  >  1:17812/1‑138 (MQ=16)                                                                      cTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTaccac                                                                                  <  1:813594/234‑1 (MQ=17)                                                                      cTTATATAGTGGGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCaa                                                                 >  1:622951/1‑251 (MQ=14)                                                                                           aTGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAgg                                                                                           <  1:504806/204‑1 (MQ=17)                                                                                                 aCCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTcc                                       <  1:866998/250‑1 (MQ=17)                                                                                                   cGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCAc                                                                             <  1:822789/210‑1 (MQ=17)                                                                                                     cTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTc                                   >  1:519975/1‑250 (MQ=17)                                                                                                     cTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGCGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTc                                   >  1:5275/1‑250 (MQ=17)                                                                                                             ctTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGGCCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCAGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAatat                                                              <  1:636028/215‑1 (MQ=12)                                                                                                             ctTTGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGAGGTACCACCGCCCTATCACCGCCAATCTTCTTTGATGACTATACTCTCTCCTTTCCGcgcttt                           >  1:579189/1‑250 (MQ=255)                                                                                                               ttGATTCCATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTAACGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGcgctttcg                         >  1:773199/1‑250 (MQ=17)                                                                                                                      cATACGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGc                                                                                                                                  >  1:707938/1‑138 (MQ=18)                                                                                                                         aCGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTc              <  1:41092/251‑1 (MQ=14)                                                                                                                         aCGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTc              <  1:349581/251‑1 (MQ=14)                                                                                                                          cGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGc                                                                                                                          >  1:173328/1‑142 (MQ=18)                                                                                                                          cGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTc              >  1:495300/1‑250 (MQ=17)                                                                                                                          cGCATAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTc              >  1:19711/1‑250 (MQ=17)                                                                                                                                gCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGttt                                                                                               >  1:314512/1‑163 (MQ=18)                                                                                                                                gCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGtt                                                                                                >  1:93509/1‑162 (MQ=18)                                                                                                                                gCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATcg                                                                        >  1:825901/1‑186 (MQ=17)                                                                                                                                gCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCtt                                     <  1:143450/221‑1 (MQ=17)                                                                                                                                gCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTc              <  1:102215/244‑1 (MQ=17)                                                                                                                                gCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTCCGCTCtt       >  1:807432/1‑251 (MQ=14)                                                                                                                                gCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTCCGCTCtt       >  1:197491/1‑251 (MQ=14)                                                                                                                                      ggTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTCCGCTCTTCGCtt  >  1:571140/1‑250 (MQ=17)                                                                                                                                          |                                                                                                                                                                                                                                                      AATCGTCATTGGCTTTACTTACCATTGACTTATCCTCCTATATCTTGAATTTGGGTTGTCAGCCTTATTCTTATATAGTGAGGATTTTTTATGTTGACCGCTTAAGCTCTTTGATTCCATACGCATAGCGTACGGTTTTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTCCGCTCTTCGCTT  >  1/1580397‑1580779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑